2024
Web-based objective and structured assessment of point-of-care lung ultrasound skills in resource-limited settings
Suttels V, Chichignoud I, Wachinou P, Du Toit J, Mans P, Blanco J, Agodokpessi G, Brahier T, Hartley M, Garcia E, Boillat-Blanco N. Web-based objective and structured assessment of point-of-care lung ultrasound skills in resource-limited settings. BMC Medical Education 2024, 24: 939. PMID: 39198828, PMCID: PMC11360711, DOI: 10.1186/s12909-024-05925-x.Peer-Reviewed Original ResearchConceptsPoint-of-care ultrasoundPOCUS trainingContinuing educationAssessment toolWeb-based assessment toolMiddle-income countriesLung point-of-care ultrasoundPractical skills testResource-limited settingsPOCUS skillsSuccess rateUltrasound skillsFischer's exact testStructural assessmentSkills TestLung ultrasound scoreWeb-basedLMICsEvaluate traineesExact testTrainingUltrasound scoreTraineesWeb-based toolSkillsAutomated Interpretation of Lung Sounds by Deep Learning in Children With Asthma: Scoping Review and Strengths, Weaknesses, Opportunities, and Threats Analysis
Ruchonnet-Métrailler I, Siebert J, Hartley M, Lacroix L. Automated Interpretation of Lung Sounds by Deep Learning in Children With Asthma: Scoping Review and Strengths, Weaknesses, Opportunities, and Threats Analysis. Journal Of Medical Internet Research 2024, 26: e53662. PMID: 39178033, PMCID: PMC11380063, DOI: 10.2196/53662.Peer-Reviewed Original ResearchConceptsGrey literature searchArtificial intelligenceScoping reviewPediatric asthmaLiterature searchManagement of pediatric asthmaField of medical AILung auscultationDeep learningMedical AIPersonalized management strategiesData collectionThreat analysisRemote monitoring capabilitiesInterpretation of lung soundsLung soundsAccuracy of AIData setsStandardized assessmentAutomated interpretationPulmonary auscultationAbsence of external validationEarly diagnosisIdentified strengthsLung sound analysisPLUS-IS-LESS project: Procalcitonin and Lung UltraSonography-based antibiotherapy in patients with Lower rESpiratory tract infection in Swiss Emergency Departments: study protocol for a pragmatic stepped-wedge cluster-randomized trial
Bessat C, Bingisser R, Schwendinger M, Bulaty T, Fournier Y, Della Santa V, Pfeil M, Schwab D, Leuppi J, Geigy N, Steuer S, Roos F, Christ M, Sirova A, Espejo T, Riedel H, Atzl A, Napieralski F, Marti J, Cisco G, Foley R, Schindler M, Hartley M, Fayet A, Garcia E, Locatelli I, Albrich W, Hugli O, Boillat-Blanco N. PLUS-IS-LESS project: Procalcitonin and Lung UltraSonography-based antibiotherapy in patients with Lower rESpiratory tract infection in Swiss Emergency Departments: study protocol for a pragmatic stepped-wedge cluster-randomized trial. Trials 2024, 25: 86. PMID: 38273319, PMCID: PMC10809691, DOI: 10.1186/s13063-023-07795-y.Peer-Reviewed Original ResearchConceptsPrescription of antibioticsRespiratory tract infectionsLung ultrasoundSwiss emergency departmentEmergency departmentTract infectionsAntibiotic prescriptionsProportion of patients prescribed antibioticsBackgroundLower respiratory tract infectionsCluster-randomizedSide effectsAntibiotic-related side effectsLower respiratory tract infectionsLength of hospital stayPatients prescribed antibioticsClinical severity scoreClinical prediction scoreProportion of patientsReducing antibiotic prescriptionsCo-primary endpointsChest X-rayDecrease side effectsPreserving patient safetyPattern of pneumoniaProportion of antibiotics
2023
Safety and immunogenicity of a synthetic nanoparticle-based, T cell priming peptide vaccine against dengue in healthy adults in Switzerland: a double-blind, randomized, vehicle-controlled, phase 1 study
Miauton A, Audran R, Besson J, Hajjami H, Karlen M, Warpelin-Decrausaz L, Sene L, Schaufelberger S, Faivre V, Faouzi M, Hartley M, Spertini F, Genton B. Safety and immunogenicity of a synthetic nanoparticle-based, T cell priming peptide vaccine against dengue in healthy adults in Switzerland: a double-blind, randomized, vehicle-controlled, phase 1 study. EBioMedicine 2023, 99: 104922. PMID: 38128414, PMCID: PMC10776924, DOI: 10.1016/j.ebiom.2023.104922.Peer-Reviewed Original ResearchConceptsCD8+ T cellsT cellsDouble-blindIntention-to-treatVehicle-controlledAdverse eventsVirus-specific CD8+ T cellsPeptide vaccinesCD8+ T cell immunityInjection site erythemaInjection site painT cell immunityPhase 1 studyPhase 1 trialDengue vaccine candidateAnti-DENV antibodiesAntibody-dependent enhancementSite painSevere dengueGlobal health threatCentral memoryPrimary outcomeCD8Microneedle injectionInduced antibodiesBarriers and facilitators to implementation of point-of-care lung ultrasonography in a tertiary centre in Benin: a qualitative study among general physicians and pneumologists
Suttels V, Da Costa S, Garcia E, Brahier T, Hartley M, Agodokpessi G, Wachinou P, Fasseur F, Boillat-Blanco N. Barriers and facilitators to implementation of point-of-care lung ultrasonography in a tertiary centre in Benin: a qualitative study among general physicians and pneumologists. BMJ Open 2023, 13: e070765. PMID: 37369423, PMCID: PMC10357755, DOI: 10.1136/bmjopen-2022-070765.Peer-Reviewed Original ResearchConceptsLower respiratory tract infectionsTertiary centerGeneral physiciansDiagnostic performanceProspective cohort studyRespiratory tract infectionsCare lung ultrasoundCare lung ultrasonographyProgram 6 monthsExcellent diagnostic performanceCohort studyImplementation of pointRespiratory symptomsTract infectionsMiddle-income countriesSpecialist careLung ultrasonographyLung ultrasoundPhysicians' perceptionsHospital settingPatient experienceQualitative studyDiagnostic approachClinical routineHealth equity
2022
Genomic Diversity of Torque Teno Virus in Blood Samples from Febrile Paediatric Outpatients in Tanzania: A Descriptive Cohort Study
Laubscher F, Hartley M, Kaiser L, Cordey S. Genomic Diversity of Torque Teno Virus in Blood Samples from Febrile Paediatric Outpatients in Tanzania: A Descriptive Cohort Study. Viruses 2022, 14: 1612. PMID: 35893678, PMCID: PMC9330782, DOI: 10.3390/v14081612.Peer-Reviewed Original ResearchConceptsTorque teno virusBlood samplesPediatric outpatientsMetagenomic next-generation sequencingFebrile pediatric outpatientsDescriptive cohort studyHuman blood viromeCohort studyIndividual blood samplesPatient ageTanzanian childrenBlood viromeSignificant associationMixed genotypesMonths/yearsTTV speciesNext-generation sequencingPositive samplesAgeOutpatientsFirst months/yearsMedianSubjectsVirusGenotypesPoint-of-care ultrasound for tuberculosis management in Sub-Saharan Africa—a balanced SWOT analysis
Suttels V, Du Toit J, Fiogbé A, Wachinou A, Guendehou B, Alovokpinhou F, Toukoui P, Hada A, Sefou F, Vinasse P, Makpemikpa G, Capo-Chichi D, Garcia E, Brahier T, Keitel K, Ouattara K, Cissoko Y, Beye S, Mans P, Agodokpessi G, Boillat-Blanco N, Hartley M. Point-of-care ultrasound for tuberculosis management in Sub-Saharan Africa—a balanced SWOT analysis. International Journal Of Infectious Diseases 2022, 123: 46-51. PMID: 35811083, DOI: 10.1016/j.ijid.2022.07.009.Peer-Reviewed Original ResearchConceptsTB managementCare ultrasoundLimitations of POCUSAdvent of ultrasoundLack of visualizationSub-Saharan AfricaTB contactTB sequelaePleural effusionLung pathologySubpleural nodulesHealthcare workersPericardial punctureTriage toolTuberculosis managementTB detectionMobile health devicesPoor specificityPOCUSUltrasoundTuberculosisObjective assessmentSmall consolidationsOperator dependencyRelevant literaturePoint-of-care lung ultrasonography for early identification of mild COVID-19: a prospective cohort of outpatients in a Swiss screening center
Schaad S, Brahier T, Hartley M, Cordonnier J, Bosso L, Espejo T, Pantet O, Hugli O, Carron P, Meuwly J, Boillat-Blanco N. Point-of-care lung ultrasonography for early identification of mild COVID-19: a prospective cohort of outpatients in a Swiss screening center. BMJ Open 2022, 12: e060181. PMID: 35750462, PMCID: PMC9234434, DOI: 10.1136/bmjopen-2021-060181.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectionLung ultrasoundClinical findingsCOVID-19Early identificationCOVID-19 positive patientsAbnormal LUS findingsBlinded independent expertsProspective observational cohortMild COVID-19Healthy healthcare workersCare lung ultrasonographyLausanne University HospitalAppropriate screening toolSARS-CoV-2COVID-19 screeningLUS featuresLUS findingsObservational cohortProspective cohortSymptom onsetAbnormal findingsClinical featuresLung ultrasonographyLung zonesUltrasound for point-of-care sputum-free tuberculosis detection: Building collaborative standardized image-banks
Suttels V, Wachinou P, Du Toit J, Boillat-Blanco N, Hartley M. Ultrasound for point-of-care sputum-free tuberculosis detection: Building collaborative standardized image-banks. EBioMedicine 2022, 81: 104078. PMID: 35649304, PMCID: PMC9160315, DOI: 10.1016/j.ebiom.2022.104078.Peer-Reviewed Original Research
2021
Blood virosphere in febrile Tanzanian children
Cordey S, Laubscher F, Hartley M, Junier T, Keitel K, Docquier M, Guex N, Iseli C, Vieille G, Le Mercier P, Gleizes A, Samaka J, Mlaganile T, Kagoro F, Masimba J, Said Z, Temba H, Elbanna G, Tapparel C, Zanella M, Xenarios I, Fellay J, D’Acremont V, Kaiser L. Blood virosphere in febrile Tanzanian children. Emerging Microbes & Infections 2021, 10: 982-993. PMID: 33929935, PMCID: PMC8171259, DOI: 10.1080/22221751.2021.1925161.Peer-Reviewed Original ResearchConceptsInfection/diseaseBlood viromeMetagenomic next-generation sequencingAcute febrile illnessLow-resource clinical settingsAvailable diagnostic toolsFebrile childrenFebrile illnessPediatric populationOutpatient departmentRoutine screeningTanzanian childrenViral infectionPotential viral originViral variantsClinical settingOne-yearDetected virusNext-generation sequencingDar es SalaamDiagnostic toolCausal virusVirusViral originDisease
2020
Clinical relevance of low-density Plasmodium falciparum parasitemia in untreated febrile children: A cohort study
Hartley M, Hofmann N, Keitel K, Kagoro F, Moniz C, Mlaganile T, Samaka J, Masimba J, Said Z, Temba H, Gonzalez I, Felger I, Genton B, D’Acremont V. Clinical relevance of low-density Plasmodium falciparum parasitemia in untreated febrile children: A cohort study. PLOS Medicine 2020, 17: e1003318. PMID: 32956354, PMCID: PMC7505590, DOI: 10.1371/journal.pmed.1003318.Peer-Reviewed Original ResearchConceptsFebrile childrenLD patientsSecondary hospitalizationCohort studyLD infectionLow-density Plasmodium infectionsMalaria rapid diagnostic testsFebrile pediatric outpatientsPlasmodium falciparum parasitemiaPrimary care levelClinical decision support algorithmPrimary care facilitiesRapid diagnostic testsHigh-density infectionsViral originPediatric outpatientsUndifferentiated feverClinical featuresClinical presentationHD patientsSevere malnutritionClinical benefitIncidental findingPlasmodium infectionPolymerase chain reactionLung Ultrasonography for Risk Stratification in Patients with Coronavirus Disease 2019 (COVID-19): A Prospective Observational Cohort Study
Brahier T, Meuwly J, Pantet O, Vez M, Donnet H, Hartley M, Hugli O, Boillat-Blanco N. Lung Ultrasonography for Risk Stratification in Patients with Coronavirus Disease 2019 (COVID-19): A Prospective Observational Cohort Study. Clinical Infectious Diseases 2020, 73: e4189-e4196. PMID: 32940646, PMCID: PMC7543331, DOI: 10.1093/cid/ciaa1408.Peer-Reviewed Original ResearchConceptsLung ultrasonographyCoronavirus disease 2019Emergency departmentLUS scoreRisk stratificationDisease 2019Lower respiratory tract infectionsProspective observational cohort studyPleural line irregularitiesEarly risk stratificationObservational cohort studyProspective observational studyRespiratory tract infectionsRisk stratification toolConfluent B-linesDifferent clinical outcomesLausanne University HospitalCOVID-19B-linesLUS characteristicsLUS findingsCohort studyTract infectionsClinical outcomesPatient group
2019
Detection of dicistroviruses RNA in blood of febrile Tanzanian children
Cordey S, Laubscher F, Hartley M, Junier T, Pérez-Rodriguez F, Keitel K, Vieille G, Samaka J, Mlaganile T, Kagoro F, Boillat-Blanco N, Mbarack Z, Docquier M, Brito F, Eibach D, May J, Sothmann P, Aldrich C, Lusingu J, Tapparel C, D’Acremont V, Kaiser L. Detection of dicistroviruses RNA in blood of febrile Tanzanian children. Emerging Microbes & Infections 2019, 8: 613-623. PMID: 30999808, PMCID: PMC6493270, DOI: 10.1080/22221751.2019.1603791.Peer-Reviewed Original ResearchConceptsTanzanian childrenViral RNA copies/Paediatric outpatient consultationsRNA copies/Higher positivity rateReal-time RT-PCRQuantitative real-time RT-PCRTanzanian adultsFebrile episodesViral loadOutpatient consultationsPositivity rateCopies/Causative pathogenNew agentsRT-PCRPlasma samplesFull-length genomeSusceptible populationChildrenViral originFurther investigationBloodSerumCapsid region
2017
Predicting Ebola infection: A malaria-sensitive triage score for Ebola virus disease
Hartley M, Young A, Tran A, Okoni-Williams H, Suma M, Mancuso B, Al-Dikhari A, Faouzi M. Predicting Ebola infection: A malaria-sensitive triage score for Ebola virus disease. PLOS Neglected Tropical Diseases 2017, 11: e0005356. PMID: 28231242, PMCID: PMC5322888, DOI: 10.1371/journal.pntd.0005356.Peer-Reviewed Original ResearchConceptsEbola virus diseaseEbola treatment centersEVD infectionTriage scoreDifferential diagnosisRetrospective cohort studyNon-specific symptomsImportant differential diagnosisPrevention/control measuresVirus diseaseCurrent triage protocolResource-poor settingsClinical characteristicsCohort studyEVD patientsUnivariate analysisNosocomial infectionsHigher oddsTriage toolPredictive scoreTreatment centersTriage protocolMultivariate analysisPatientsDifferential diagnosticsPredicting Ebola Severity: A Clinical Prioritization Score for Ebola Virus Disease
Hartley M, Young A, Tran A, Okoni-Williams H, Suma M, Mancuso B, Al-Dikhari A, Faouzi M. Predicting Ebola Severity: A Clinical Prioritization Score for Ebola Virus Disease. PLOS Neglected Tropical Diseases 2017, 11: e0005265. PMID: 28151955, PMCID: PMC5289426, DOI: 10.1371/journal.pntd.0005265.Peer-Reviewed Original ResearchConceptsEbola virus diseaseViral loadPrognostic toolEbola viral loadMultivariate prognostic scoreRetrospective cohort studyEbola treatment centersAccurate prognostic toolVirus diseaseEVD mortalityClinical characteristicsCohort studyPrognostic scoreAsymptomatic infectionClinical severityIntensive treatmentPrognostic potentialUnadjusted predictorsDisease scoreMortality rateTreatment centersEVD diagnosisMortality risk reductionDeadly infectionEarly reportingExacerbated Leishmaniasis Caused by a Viral Endosymbiont can be Prevented by Immunization with Its Viral Capsid
Castiglioni P, Hartley M, Rossi M, Prevel F, Desponds C, Utzschneider D, Eren R, Zangger H, Brunner L, Collin N, Zehn D, Kuhlmann F, Beverley S, Fasel N, Ronet C. Exacerbated Leishmaniasis Caused by a Viral Endosymbiont can be Prevented by Immunization with Its Viral Capsid. PLOS Neglected Tropical Diseases 2017, 11: e0005240. PMID: 28099431, PMCID: PMC5242429, DOI: 10.1371/journal.pntd.0005240.Peer-Reviewed Original ResearchConceptsSymptomatic relapseVaccinated miceVaccine strategiesPotent innate immunogenSimilar viral infectionsVirus-related complicationsT-cell transferSpecific T cellsPoor treatment responseLesional inflammationSerum transferT helperInitial treatmentC57BL/6 miceNaïve miceLeishmania infectionPreventive benefitsVaccine opportunitiesT cellsTreatment responseImmune responseViral infectionLesion sizeClinical effortsLeishmania guyanensis
2016
Severe Cutaneous Leishmaniasis in a Human Immunodeficiency Virus Patient Coinfected with Leishmania braziliensis and Its Endosymbiotic Virus
Parmentier L, Cusini A, Müller N, Zangger H, Hartley M, Desponds C, Castiglioni P, Dubach P, Ronet C, Beverley S, Fasel N. Severe Cutaneous Leishmaniasis in a Human Immunodeficiency Virus Patient Coinfected with Leishmania braziliensis and Its Endosymbiotic Virus. American Journal Of Tropical Medicine And Hygiene 2016, 94: 840-843. PMID: 26834198, PMCID: PMC4824227, DOI: 10.4269/ajtmh.15-0803.Peer-Reviewed Original ResearchConceptsHuman immunodeficiency virusLeishmania infectionCutaneous leishmaniasisPresence of LRV1Primary skin lesionsDrug treatment failureAggressive pathogenesisAntiretroviral therapyHIV patientsSpecies of LeishmaniaMurine leishmaniasisTreatment failureImmunodeficiency virusNasal lesionsRisk factorsEmergence of leishmaniasisSkin lesionsCutaneous afflictionsFurther exacerbationDisease severityLeishmania parasitesLeishmaniasisLeishmania braziliensisLRV1Patients
2015
Presence of Leishmania RNA Virus 1 in Leishmania guyanensis Increases the Risk of First-Line Treatment Failure and Symptomatic Relapse
Bourreau E, Ginouves M, Prévot G, Hartley M, Gangneux J, Robert-Gangneux F, Dufour J, Sainte-Marie D, Bertolotti A, Pratlong F, Martin R, Schütz F, Couppié P, Fasel N, Ronet C. Presence of Leishmania RNA Virus 1 in Leishmania guyanensis Increases the Risk of First-Line Treatment Failure and Symptomatic Relapse. The Journal Of Infectious Diseases 2015, 213: 105-111. PMID: 26123564, DOI: 10.1093/infdis/jiv355.Peer-Reviewed Original ResearchConceptsL. guyanensis infectionLeishmania RNA virus 1American tegumentary leishmaniasisSymptomatic relapseTreatment failureTegumentary leishmaniasisFirst-line treatment failureDoses of pentamidineLeishmania guyanensis infectionLine treatment failureFirst-line treatmentChronic cutaneous leishmaniasisVirus 1Second-line drugsExperimental murine leishmaniasisInflammatory markersHyperinflammatory responseExtended therapyMurine leishmaniasisTherapeutic choiceSuch complicationsRNA virus 1Persistent infectionCutaneous leishmaniasisRelapse
2014
The immunological, environmental, and phylogenetic perpetrators of metastatic leishmaniasis
Hartley M, Drexler S, Ronet C, Beverley S, Fasel N. The immunological, environmental, and phylogenetic perpetrators of metastatic leishmaniasis. Trends In Parasitology 2014, 30: 412-422. PMID: 24954794, PMCID: PMC4287268, DOI: 10.1016/j.pt.2014.05.006.Peer-Reviewed Original ResearchConceptsInnate immune systemImmune systemSelf-healing cutaneous lesionsPattern recognition receptorsFacial mucosaMetastatic leishmaniasisMetastatic diseasePapular rashCutaneous lesionsSuch diverse pathologiesCutaneous systemHost immunocompetenceGranulomatous nodulesParasitic infectionsRecognition receptorsPotential associationCutaneous leishmaniasesDiverse pathologiesMillions of peoplePathologyMultiple secondary sitesSecondary sitesRashUlcerationMetastasis
2013
The therapeutic potential of immune cross‐talk in leishmaniasis
Hartley M, Kohl K, Ronet C, Fasel N. The therapeutic potential of immune cross‐talk in leishmaniasis. Clinical Microbiology And Infection 2013, 19: 119-130. PMID: 23398405, DOI: 10.1111/1469-0691.12095.Peer-Reviewed Original ResearchConceptsMetastatic leishmaniasisCutaneous leishmaniasisLeishmania parasitesSelf-healing lesionsProtozoan parasitic diseaseEffective antileishmanial drugsSpecies of LeishmaniaDestructive lesionsImmune homeostasisCurrent treatmentTherapeutic strategiesImmune pathwaysNew drug targetsTherapeutic potentialParasitic diseasesAntileishmanial drugsLeishmaniasisLesionsPrevalent formDiseaseDrug targetsRNA virusesTreatmentVariable effectsParasites