2024
Corrigendum: Identification of genes influencing the evolution of Escherichia coli ST372 in dogs and humans.
Elankumaran P, Browning G, Marenda M, Kidsley A, Osman M, Haenni M, Johnson J, Trott D, Reid C, Djordjevic S. Corrigendum: Identification of genes influencing the evolution of Escherichia coli ST372 in dogs and humans. Microbial Genomics 2024, 10 PMID: 39166982, PMCID: PMC11561579, DOI: 10.1099/mgen.0.001286.Peer-Reviewed Original ResearchConceptsIdentification of genes
2023
First report of the mobile colistin resistance gene, mcr-1.26, in multidrug-resistant Escherichia coli isolated from retail chicken meat
Kassem I, Osman M, Hassan J, Sulaiman A, Mann D, Esseili M, Naas T, Deng X. First report of the mobile colistin resistance gene, mcr-1.26, in multidrug-resistant Escherichia coli isolated from retail chicken meat. Journal Of Global Antimicrobial Resistance 2023, 34: 176-178. PMID: 37429544, DOI: 10.1016/j.jgar.2023.07.005.Peer-Reviewed Original ResearchConceptsMinimum inhibitory concentrationColistin resistanceColistin-resistant Escherichia coliKirby-Bauer disk diffusionMultidrug-resistant Escherichia coliMDR E. coliPlasmid replicon typesWhole-genome sequencingSequence typesColistin resistance geneRetail chicken meatBroth microdilution methodMobile colistin resistance geneReplicon typesSusceptibility testingIncX4 plasmidsFirst reportMultidrug resistanceWhole-genome sequencing analysisDepth phenotypicInhibitory concentrationDisk diffusionMicrodilution methodGenomic epidemiologyAntimicrobial resistance genesIdentification of genes influencing the evolution of Escherichia coli ST372 in dogs and humans
Elankumuran P, Browning G, Marenda M, Kidsley A, Osman M, Haenni M, Johnson J, Trott D, Reid C, Djordjevic S. Identification of genes influencing the evolution of Escherichia coli ST372 in dogs and humans. Microbial Genomics 2023, 9: mgen000930. PMID: 36752777, PMCID: PMC9997745, DOI: 10.1099/mgen.0.000930.Peer-Reviewed Original ResearchConceptsPopulation structureIdentification of genesWhole genome sequencesPropanediol utilization (pdu) operonGenomic traitsAccessory genomeCanine sourcesBioinformatics analysisUtilization operonPhylogenetic clustersGlobal collectionDistinct populationsVirulence genesOperonGenesDominant hostSequence typesSequenceExtraintestinal infectionsPhylogenyCladeGenomeLineagesHumansTraits
2022
Antimicrobial resistance trends among canine Escherichia coli isolated at a New York veterinary diagnostic laboratory between 2007 and 2020
Osman M, Albarracin B, Altier C, Gröhn Y, Cazer C. Antimicrobial resistance trends among canine Escherichia coli isolated at a New York veterinary diagnostic laboratory between 2007 and 2020. Preventive Veterinary Medicine 2022, 208: 105767. PMID: 36181749, PMCID: PMC9703301, DOI: 10.1016/j.prevetmed.2022.105767.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Bacterial AgentsAnti-Infective AgentsCephalosporinsDog DiseasesDogsDrug Resistance, BacterialEnrofloxacinEscherichia coliEscherichia coli InfectionsFluoroquinolonesGentamicinsMicrobial Sensitivity TestsNew YorkRetrospective StudiesTrimethoprim, Sulfamethoxazole Drug CombinationConceptsTrimethoprim-sulfamethoxazole resistanceBody sitesGood first-line choiceCanine urinary tract infectionsResistant isolatesResistance trendsLaboratory Standards Institute breakpointsMultivariable logistic regression modelAntimicrobial resistance surveillance studyUrinary tract infectionDrug-resistant Escherichia coliFirst-line choiceAvailable clinical dataDrug-resistant E. coliAntimicrobial susceptibility patternsRetrospective study designUse of fluoroquinolonesE. coli infectionAntimicrobial resistance trendsResistance surveillance studiesClinical E. coliLogistic regression modelsEnrofloxacin-resistant isolatesSurvival analysis dataMIC trendsLetter to the Editor: First Report of the Detection of the Plasmid-Borne Colistin Resistance Gene, mcr-1.26, in Multidrug-Resistant Escherichia coli Isolated from a Domesticated Pigeon
Kassem I, Assi A, Osman M, Mann D, Li S, Deng X. Letter to the Editor: First Report of the Detection of the Plasmid-Borne Colistin Resistance Gene, mcr-1.26, in Multidrug-Resistant Escherichia coli Isolated from a Domesticated Pigeon. Microbial Drug Resistance 2022, 28: 821-823. PMID: 35639443, DOI: 10.1089/mdr.2021.0359.Peer-Reviewed Original ResearchGenome sequence of a multidrug-resistant Campylobacter coli strain isolated from a newborn with severe diarrhea in Lebanon
Halimeh F, Rafei R, Diene S, Osman M, Kassem I, Jamal Akoum R, Moudani W, Hamze M, Rolain J. Genome sequence of a multidrug-resistant Campylobacter coli strain isolated from a newborn with severe diarrhea in Lebanon. Folia Microbiologica 2022, 67: 319-328. PMID: 34997523, DOI: 10.1007/s12223-021-00921-w.Peer-Reviewed Original ResearchConceptsCampylobacter coli strainsSevere diarrheaST-828 complexC. coli isolatesBurden of CampylobacterBeta-lactam resistanceNew sequence typesSequencing analysisEpidemiological backgroundColi isolatesWhole-genome sequencing analysisAminoglycoside resistanceC. coliColi strainsAntimicrobial resistanceBlaOXA-61Virulence determinantsDisk diffusionMLST analysisWGS analysisDiarrheaNewbornsPTet plasmidAntimicrobial susceptibility phenotypesSusceptibility phenotype
2020
Endocannabinoids Attenuate the Virulence of Certain Enteropathogenic Bacteria
Osman M, Papon N, Weill F. Endocannabinoids Attenuate the Virulence of Certain Enteropathogenic Bacteria. Trends In Microbiology 2020, 29: 185-187. PMID: 33388214, DOI: 10.1016/j.tim.2020.12.008.Peer-Reviewed Original ResearchConceptsEndocannabinoid systemInflammatory bowel diseaseIntestinal bacterial infectionsMammalian endocannabinoid systemBowel diseaseEnteric infectionsIntestinal infectionsLipid hormonesGastrointestinal physiologyBacterial infectionsEnteropathogenic bacteriaInfectionPromising targetPossible benefitsNew findingsDiseaseMiceHormoneImmunityCannabinoids
2019
Prevalence and genetic diversity of Campylobacter spp. in the production chain of broiler chickens in Lebanon and its association with the intestinal protozoan Blastocystis sp
Greige S, Rivoal K, Osman M, Safadi D, Dabboussi F, Hage R, Viscogliosi E, Hamze M, Chemaly M. Prevalence and genetic diversity of Campylobacter spp. in the production chain of broiler chickens in Lebanon and its association with the intestinal protozoan Blastocystis sp. Poultry Science 2019, 98: 5883-5891. PMID: 31134283, DOI: 10.3382/ps/pez286.Peer-Reviewed Original ResearchConceptsHigh prevalenceCampylobacter sppBlastocystis spBroiler chickensC. coliC. jejuniBurden of campylobacteriosisC. jejuni isolatesPublic health impactHuman clinical casesPrevalence of CampylobacterSignificant associationJejuni isolatesSlaughterhouse workersClinical casesPrevalenceHuman campylobacteriosisCecumHuman gastroenteritisCampylobacter jejuniCampylobacter speciesCommon foodborne pathogensHealth impactsRecent dataTyping methodsPlasmid-mediated quinolone resistance: Mechanisms, detection, and epidemiology in the Arab countries
Yassine I, Rafei R, Osman M, Mallat H, Dabboussi F, Hamze M. Plasmid-mediated quinolone resistance: Mechanisms, detection, and epidemiology in the Arab countries. Infection Genetics And Evolution 2019, 76: 104020. PMID: 31493557, DOI: 10.1016/j.meegid.2019.104020.Peer-Reviewed Original ResearchConceptsQuinolone resistance mechanismsQuinolone resistancePlasmid-mediated quinolone resistance mechanismsPlasmid-mediated genesImportant antimicrobial classesResistance mechanismsTreatment failureEnterobacterial infectionsPMQR genesStewardship effortsAntimicrobial classesPMQRIrrational useMultiple mechanismsHigh rateEpidemiologyQuinolonesCommon associationCrucial antibioticsEnvironmental isolatesMethod of detectionEnterobacteriaceaeAntibiotic resistance genesAnimalsResistance genesMolecular epidemiology of Campylobacter isolates from broiler slaughterhouses in Tripoli, North of Lebanon
Rafei R, Al Kassaa I, Osman M, Dabboussi F, Hamze M. Molecular epidemiology of Campylobacter isolates from broiler slaughterhouses in Tripoli, North of Lebanon. British Poultry Science 2019, 60: 675-682. PMID: 31321996, DOI: 10.1080/00071668.2019.1645945.Peer-Reviewed Original ResearchMeSH KeywordsAbattoirsAnimalsAnti-Bacterial AgentsCampylobacterCampylobacter InfectionsCecumChickensDrug Resistance, BacterialElectrophoresis, Gel, Pulsed-FieldFlagellinFoodborne DiseasesGenetic VariationHumansLebanonPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthPoultry DiseasesPrevalenceSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationConceptsAntibiotic resistance profilesErtapenem resistanceMolecular epidemiologyReal burdenResistance profilesAntibiotic resistanceRestriction fragment length polymorphismNorth of LebanonFragment length polymorphismEpidemiologyField gel electrophoresisCampylobacterLength polymorphismBroiler slaughterhousesBroilersIsolatesSlaughterhouseGentamicinAmoxicillinErythromycinFirst report on the prevalence and subtype distribution of Blastocystis sp. in dairy cattle in Lebanon and assessment of zoonotic transmission
Greige S, El Safadi D, Khaled S, Gantois N, Baydoun M, Chemaly M, Benamrouz-Vanneste S, Chabé M, Osman M, Certad G, Hamze M, Viscogliosi E. First report on the prevalence and subtype distribution of Blastocystis sp. in dairy cattle in Lebanon and assessment of zoonotic transmission. Acta Tropica 2019, 194: 23-29. PMID: 30878470, DOI: 10.1016/j.actatropica.2019.02.013.Peer-Reviewed Original ResearchConceptsDomestic cattleBlastocystis spCattle livestockDairy cattleFecal samplesLow host specificityNon-human hostsDairy farmersZoonotic potentialZoonotic transmissionCattleZoonotic reservoirsFarmers' CohortLivestockAnimal hostsST3 isolatesPossible reservoirST distributionHost specificityST10FarmsHuman populationHuman infectionsPositive samplesST1
2018
Epidemiology of antimicrobial resistance in Lebanese extra-hospital settings: An overview
Osman M, Al Mir H, Rafei R, Dabboussi F, Madec J, Haenni M, Hamze M. Epidemiology of antimicrobial resistance in Lebanese extra-hospital settings: An overview. Journal Of Global Antimicrobial Resistance 2018, 17: 123-129. PMID: 30553113, DOI: 10.1016/j.jgar.2018.11.019.Peer-Reviewed Original ResearchConceptsAntimicrobial resistanceExtra-hospital settingsTerms of mortalityMiracle drugAntimicrobial-resistant pathogensNon-clinical settingsSignificant morbidityClinical settingNovel drugsAntimicrobial-resistant bacteriaMultidrug-resistant bacterial strainsSocietal costsInappropriate useCombat infectionEpidemiological situationMorbidityMortalityEpidemiologyDrugsAntibioticsVeterinary medicineAMR crisisMajority of investigations
2017
Prevalence, transmission, and host specificity of Cryptosporidium spp. in various animal groups from two French zoos
Osman M, El Safadi D, Benamrouz-Vanneste S, Cian A, Moriniere R, Gantois N, Delgado-Viscogliosi P, Guyot K, Bosc S, Chabé M, Petit T, Viscogliosi E, Certad G. Prevalence, transmission, and host specificity of Cryptosporidium spp. in various animal groups from two French zoos. Parasitology Research 2017, 116: 3419-3422. PMID: 29030716, DOI: 10.1007/s00436-017-5645-1.Peer-Reviewed Original ResearchConceptsCryptosporidium sppCaptivity animalsFrench zoosSpecies of CryptosporidiumPrevalence of CryptosporidiumFecal samplesC. galliC. tyzzeriC. andersoniCryptosporidium speciesFirst molecular studyCryptosporidium DNACryptosporidium infectionHost specificityPotential reservoirsDifferent hostsCryptosporidiumMolecular studiesZoological gardensSpeciesAnimal groupsSppAnimalsParasitesAndersoniMolecular Epidemiology of Blastocystis sp. in Various Animal Groups from Two French Zoos and Evaluation of Potential Zoonotic Risk
Cian A, Safadi D, Osman M, Moriniere R, Gantois N, Benamrouz-Vanneste S, Delgado-Viscogliosi P, Guyot K, Li L, Monchy S, Noël C, Poirier P, Nourrisson C, Wawrzyniak I, Delbac F, Bosc S, Chabé M, Petit T, Certad G, Viscogliosi E. Molecular Epidemiology of Blastocystis sp. in Various Animal Groups from Two French Zoos and Evaluation of Potential Zoonotic Risk. PLOS ONE 2017, 12: e0169659. PMID: 28060901, PMCID: PMC5217969, DOI: 10.1371/journal.pone.0169659.Peer-Reviewed Original ResearchConceptsAnimal hostsAnimal groupsFrench zoosNon-mammalian speciesNon-mammalian groupsBlastocystis spExtensive genetic diversityZoonotic transmissionNew animal hostsGenetic diversityHost specificityHuman infectionsAvian hostsPotential zoonotic riskReal-time PCRSpIntensive samplingInsectsSpeciesZoonotic riskReptilesHuman populationAnimal handlersAvian subtypesCommon intestinal parasites
2016
Prevalence and Risk Factors for Intestinal Protozoan Infections with Cryptosporidium, Giardia, Blastocystis and Dientamoeba among Schoolchildren in Tripoli, Lebanon
Osman M, Safadi D, Cian A, Benamrouz S, Nourrisson C, Poirier P, Pereira B, Razakandrainibe R, Pinon A, Lambert C, Wawrzyniak I, Dabboussi F, Delbac F, Favennec L, Hamze M, Viscogliosi E, Certad G. Prevalence and Risk Factors for Intestinal Protozoan Infections with Cryptosporidium, Giardia, Blastocystis and Dientamoeba among Schoolchildren in Tripoli, Lebanon. PLOS Neglected Tropical Diseases 2016, 10: e0004496. PMID: 26974335, PMCID: PMC4790957, DOI: 10.1371/journal.pntd.0004496.Peer-Reviewed Original ResearchConceptsIntestinal protozoan infectionsProtozoan infectionsRisk factorsAssemblages of GiardiaGeneral pediatric populationPrimary risk factorCross-sectional studyPersonal hygiene habitsIntestinal pathogenic protozoaMolecular epidemiological dataLogistic regression modelsHigher infection rateSubtypes of BlastocystisDigestive symptomsPediatric populationGastrointestinal disordersAsymptomatic infectionAsymptomatic subjectsHygiene habitsOverall prevalenceStool samplesHigh prevalenceEpidemiological dataHigh riskDientamoeba fragilis
2015
Prevalence and genetic diversity of the intestinal parasites Blastocystis sp. and Cryptosporidium spp. in household dogs in France and evaluation of zoonotic transmission risk
Osman M, Bories J, Safadi D, Poirel M, Gantois N, Benamrouz-Vanneste S, Delhaes L, Hugonnard M, Certad G, Zenner L, Viscogliosi E. Prevalence and genetic diversity of the intestinal parasites Blastocystis sp. and Cryptosporidium spp. in household dogs in France and evaluation of zoonotic transmission risk. Veterinary Parasitology 2015, 214: 167-170. PMID: 26395822, DOI: 10.1016/j.vetpar.2015.09.015.Peer-Reviewed Original ResearchConceptsCryptosporidium sppHousehold dogsBlastocystis spC. canisCanine populationZoonotic transmission riskFecal samplesZoonotic riskGastrointestinal parasitesDomestic dogsZoonotic reservoirsGenetic diversityCystoisospora spGastrointestinal symptomsCommon parasiteLight microscopy examinationNatural hostOverall prevalenceSppLow prevalenceSubtype 2Giardia spIntestinal parasitesIntestinal protozoaMolecular epidemiologyIdentification of Cryptosporidium Species in Fish from Lake Geneva (Lac Léman) in France
Certad G, Dupouy-Camet J, Gantois N, Hammouma-Ghelboun O, Pottier M, Guyot K, Benamrouz S, Osman M, Delaire B, Creusy C, Viscogliosi E, Dei-Cas E, Aliouat-Denis C, Follet J. Identification of Cryptosporidium Species in Fish from Lake Geneva (Lac Léman) in France. PLOS ONE 2015, 10: e0133047. PMID: 26213992, PMCID: PMC4516323, DOI: 10.1371/journal.pone.0133047.Peer-Reviewed Original ResearchConceptsC. molnariC. parvumCryptosporidium speciesEdible freshwater fishDigestive epithelial cellsFreshwater fishZoonotic speciesCryptosporidium sppCryptosporidium subtypesWildlife speciesGp60 subtypesFish speciesTerrestrial mammalsCoregonus lavaretusDegenerate primersEntire fishPotential hostsSequence analysisPerca fluviatilisRutilus rutilusSalvelinus alpinusEsox luciusDevelopmental stagesParvumLake Geneva
2014
Cryptosporidium parvum-induced ileo-caecal adenocarcinoma and Wnt signaling in a mouse model
Benamrouz S, Conseil V, Chabé M, Praet M, Audebert C, Blervaque R, Guyot K, Gazzola S, Mouray A, Chassat T, Delaire B, Goetinck N, Gantois N, Osman M, Slomianny C, Dehennaut V, Lefebvre T, Viscogliosi E, Cuvelier C, Dei-Cas E, Creusy C, Certad G. Cryptosporidium parvum-induced ileo-caecal adenocarcinoma and Wnt signaling in a mouse model. Disease Models & Mechanisms 2014, 7: 693-700. PMID: 24652769, PMCID: PMC4036476, DOI: 10.1242/dmm.013292.Peer-Reviewed Original ResearchConceptsHost cell biological processesCell migrationHigh-throughput sequencingHost-pathogen interactionsPotential tumorigenic roleCytoskeleton activityAnimal modelsAdherens junctionsCytoskeleton networkC. parvum-infected micePathway componentsBiological processesApicomplexan protozoaCell cycleStudied genesInfection processMetabolic pathwaysSelf-limiting diarrheaReproducible animal modelC. parvumAdult SCID miceLife-threatening diseaseΒ-cateninTumorigenic roleCryptosporidium species