Featured Publications
Machine-learning approaches prevent post-treatment resistance-gaining bacterial recurrences
Osman M, Mahieu R, Eveillard M. Machine-learning approaches prevent post-treatment resistance-gaining bacterial recurrences. Trends In Microbiology 2022, 30: 612-614. PMID: 35599112, DOI: 10.1016/j.tim.2022.05.006.Peer-Reviewed Original ResearchAntimicrobial resistance in the protracted Syrian conflict: halting a war in the war
Osman M, Rafei R, Ismail M, Al Omari S, Mallat H, Dabboussi F, Cazer C, Karah N, Abbara A, Hamze M. Antimicrobial resistance in the protracted Syrian conflict: halting a war in the war. Future Microbiology 2021, 16: 825-845. PMID: 34223789, DOI: 10.2217/fmb-2021-0040.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Bacterial AgentsArmed ConflictsDrug Resistance, BacterialEuropeHealth PolicyRefugeesSyriaConceptsSyrian crisisSyrian conflictMassive displacementHost countryEffective policiesMiddle EastInternational health organizationsWarExhausting conditionsConflictCrisisCurrent situationCurrent obstaclesKey interventionsRefugeesGovernmentPolicySyriaCountriesEuropeContextOrganizationEastThreatEmergence
2023
Genomic analysis of Shigella isolates from Lebanon reveals marked genetic diversity and antimicrobial resistance
Yassine I, Rafei R, de la Gandara M, Osman M, Fabre L, Dabboussi F, Hamze M, Weill F. Genomic analysis of Shigella isolates from Lebanon reveals marked genetic diversity and antimicrobial resistance. Microbial Genomics 2023, 9: 001157. PMID: 38100171, PMCID: PMC10763507, DOI: 10.1099/mgen.0.001157.Peer-Reviewed Original ResearchConceptsExtended-spectrum beta-lactamase genesThird-generation cephalosporinsMultidrug resistance phenotypeBeta-lactamase genesAntimicrobial resistance determinantsPrevalent serogroupsClinical isolatesQuinolone resistanceDNA gyrase genesAntimicrobial resistanceResistance determinantsCTXResistance phenotypeAmino acid substitutionsGyrase genesIsolatesNorth LebanonFirst studyAcid substitutionsPoint mutationsGenomic analysisCephalosporins
2022
Antimicrobial resistance trends among canine Escherichia coli isolated at a New York veterinary diagnostic laboratory between 2007 and 2020
Osman M, Albarracin B, Altier C, Gröhn Y, Cazer C. Antimicrobial resistance trends among canine Escherichia coli isolated at a New York veterinary diagnostic laboratory between 2007 and 2020. Preventive Veterinary Medicine 2022, 208: 105767. PMID: 36181749, PMCID: PMC9703301, DOI: 10.1016/j.prevetmed.2022.105767.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Bacterial AgentsAnti-Infective AgentsCephalosporinsDog DiseasesDogsDrug Resistance, BacterialEnrofloxacinEscherichia coliEscherichia coli InfectionsFluoroquinolonesGentamicinsMicrobial Sensitivity TestsNew YorkRetrospective StudiesTrimethoprim, Sulfamethoxazole Drug CombinationConceptsTrimethoprim-sulfamethoxazole resistanceBody sitesGood first-line choiceCanine urinary tract infectionsResistant isolatesResistance trendsLaboratory Standards Institute breakpointsMultivariable logistic regression modelAntimicrobial resistance surveillance studyUrinary tract infectionDrug-resistant Escherichia coliFirst-line choiceAvailable clinical dataDrug-resistant E. coliAntimicrobial susceptibility patternsRetrospective study designUse of fluoroquinolonesE. coli infectionAntimicrobial resistance trendsResistance surveillance studiesClinical E. coliLogistic regression modelsEnrofloxacin-resistant isolatesSurvival analysis dataMIC trendsHigh rates of macrolide and fluoroquinolone resistance in human campylobacteriosis in the Middle East and North Africa
Dabbousi A, Osman M, Dabboussi F, Hamze M. High rates of macrolide and fluoroquinolone resistance in human campylobacteriosis in the Middle East and North Africa. Future Microbiology 2022, 17: 957-967. PMID: 35796073, DOI: 10.2217/fmb-2021-0302.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Bacterial AgentsCampylobacter InfectionsCampylobacter jejuniDrug Resistance, BacterialFluoroquinolonesHumansIntraabdominal InfectionsLebanonMacrolidesMicrobial Sensitivity TestsConceptsHuman campylobacteriosisRoutine stool cultureBacterial gastrointestinal infectionsClinical laboratoriesIncidence of campylobacteriosisPaucity of dataMain causative agentStool culturesGastrointestinal infectionsDiarrheal patientsMicrobiological diagnosisClinical importanceFluoroquinolone resistanceInfection prevalenceAntimicrobial resistanceZoonotic diseaseCausative agentCampylobacteriosisHigh rateAlarming ratePatientsCritical needEpidemiologyInfectionPrevalenceLetter to the Editor: First Report of the Detection of the Plasmid-Borne Colistin Resistance Gene, mcr-1.26, in Multidrug-Resistant Escherichia coli Isolated from a Domesticated Pigeon
Kassem I, Assi A, Osman M, Mann D, Li S, Deng X. Letter to the Editor: First Report of the Detection of the Plasmid-Borne Colistin Resistance Gene, mcr-1.26, in Multidrug-Resistant Escherichia coli Isolated from a Domesticated Pigeon. Microbial Drug Resistance 2022, 28: 821-823. PMID: 35639443, DOI: 10.1089/mdr.2021.0359.Peer-Reviewed Original ResearchA global snapshot on the prevalent macrolide-resistant emm types of Group A Streptococcus worldwide, their phenotypes and their resistance marker genotypes during the last two decades: A systematic review
Rafei R, Al Iaali R, Osman M, Dabboussi F, Hamze M. A global snapshot on the prevalent macrolide-resistant emm types of Group A Streptococcus worldwide, their phenotypes and their resistance marker genotypes during the last two decades: A systematic review. Infection Genetics And Evolution 2022, 99: 105258. PMID: 35219865, DOI: 10.1016/j.meegid.2022.105258.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Bacterial AgentsBacterial Outer Membrane ProteinsBiomarkersDrug Resistance, BacterialGenotypeHumansMacrolidesPhenotypeStreptococcal InfectionsStreptococcus pyogenesConceptsEmm typesMacrolide resistance phenotypeEmm clustersMacrolide resistanceSystematic reviewPeculiar epidemiological featuresPresent systematic reviewEmm pattern AGroup A StreptococcusResistance phenotypeClinical presentationMiddle-income countriesEpidemiological featuresHigh-income countriesExclusion criteriaMacrolide susceptibilityA StreptococcusEpidemiological surveillanceSustained surveillancePattern EPattern AIncome countriesPhenotypeSurveillanceEpidemiological dynamicsGenome sequence of a multidrug-resistant Campylobacter coli strain isolated from a newborn with severe diarrhea in Lebanon
Halimeh F, Rafei R, Diene S, Osman M, Kassem I, Jamal Akoum R, Moudani W, Hamze M, Rolain J. Genome sequence of a multidrug-resistant Campylobacter coli strain isolated from a newborn with severe diarrhea in Lebanon. Folia Microbiologica 2022, 67: 319-328. PMID: 34997523, DOI: 10.1007/s12223-021-00921-w.Peer-Reviewed Original ResearchConceptsCampylobacter coli strainsSevere diarrheaST-828 complexC. coli isolatesBurden of CampylobacterBeta-lactam resistanceNew sequence typesSequencing analysisEpidemiological backgroundColi isolatesWhole-genome sequencing analysisAminoglycoside resistanceC. coliColi strainsAntimicrobial resistanceBlaOXA-61Virulence determinantsDisk diffusionMLST analysisWGS analysisDiarrheaNewbornsPTet plasmidAntimicrobial susceptibility phenotypesSusceptibility phenotype
2021
Shortage of appropriate diagnostics for antimicrobial resistance in Lebanese clinical settings: a crisis amplified by COVID-19 and economic collapse
Osman M, Kasir D, Kassem I, Hamze M. Shortage of appropriate diagnostics for antimicrobial resistance in Lebanese clinical settings: a crisis amplified by COVID-19 and economic collapse. Journal Of Global Antimicrobial Resistance 2021, 27: 72-74. PMID: 34492397, PMCID: PMC8488586, DOI: 10.1016/j.jgar.2021.08.008.Peer-Reviewed Original Research
2020
Molecular epidemiology of nonpharyngeal group A streptococci isolates in northern Lebanon
Rafei R, Hawli M, Osman M, Khelissa S, Salloum T, Dabboussi F, Tokajian S, Hamze M. Molecular epidemiology of nonpharyngeal group A streptococci isolates in northern Lebanon. Future Microbiology 2020, 15: 1555-1569. PMID: 33236928, DOI: 10.2217/fmb-2020-0072.Peer-Reviewed Original ResearchDistribution of emm types and macrolide resistance determinants among group A streptococci in the Middle East and North Africa region
Rafei R, Hawli M, Osman M, Dabboussi F, Hamze M. Distribution of emm types and macrolide resistance determinants among group A streptococci in the Middle East and North Africa region. Journal Of Global Antimicrobial Resistance 2020, 22: 334-348. PMID: 32084609, DOI: 10.1016/j.jgar.2020.02.005.Peer-Reviewed Original ResearchMeSH KeywordsAfrica, NorthernAnti-Bacterial AgentsDrug Resistance, BacterialGenotypeHumansMacrolidesMiddle EastConceptsInvasive GAS infectionsGAS infectionVaccine candidatesProtein-based vaccine candidatesEpidemiology of groupMacrolide resistance determinantsMacrolide resistance profilesGAS prevalencePharyngitis patientsDisease burdenEmm typesGAS isolatesHealthy carriersSystematic surveillanceResistance profilesErythromycin resistancePotential coverageBurdenResistance determinantsInfectionStreptococciCoverage rateEmm28Emm4Emm89
2019
Plasmid-mediated quinolone resistance: Mechanisms, detection, and epidemiology in the Arab countries
Yassine I, Rafei R, Osman M, Mallat H, Dabboussi F, Hamze M. Plasmid-mediated quinolone resistance: Mechanisms, detection, and epidemiology in the Arab countries. Infection Genetics And Evolution 2019, 76: 104020. PMID: 31493557, DOI: 10.1016/j.meegid.2019.104020.Peer-Reviewed Original ResearchConceptsQuinolone resistance mechanismsQuinolone resistancePlasmid-mediated quinolone resistance mechanismsPlasmid-mediated genesImportant antimicrobial classesResistance mechanismsTreatment failureEnterobacterial infectionsPMQR genesStewardship effortsAntimicrobial classesPMQRIrrational useMultiple mechanismsHigh rateEpidemiologyQuinolonesCommon associationCrucial antibioticsEnvironmental isolatesMethod of detectionEnterobacteriaceaeAntibiotic resistance genesAnimalsResistance genesMolecular epidemiology of Campylobacter isolates from broiler slaughterhouses in Tripoli, North of Lebanon
Rafei R, Kassaa I, Osman M, Dabboussi F, Hamze M. Molecular epidemiology of Campylobacter isolates from broiler slaughterhouses in Tripoli, North of Lebanon. British Poultry Science 2019, 60: 675-682. PMID: 31321996, DOI: 10.1080/00071668.2019.1645945.Peer-Reviewed Original ResearchMeSH KeywordsAbattoirsAnimalsAnti-Bacterial AgentsCampylobacterCampylobacter InfectionsCecumChickensDrug Resistance, BacterialElectrophoresis, Gel, Pulsed-FieldFlagellinFoodborne DiseasesGenetic VariationHumansLebanonPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthPoultry DiseasesPrevalenceSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationConceptsAntibiotic resistance profilesErtapenem resistanceMolecular epidemiologyReal burdenResistance profilesAntibiotic resistanceRestriction fragment length polymorphismNorth of LebanonFragment length polymorphismEpidemiologyField gel electrophoresisCampylobacterLength polymorphismBroiler slaughterhousesBroilersIsolatesSlaughterhouseGentamicinAmoxicillinErythromycin
2017
Molecular mechanisms and epidemiology of resistance in Streptococcus pneumoniae in the Middle East region
El Moujaber G, Osman M, Rafei R, Dabboussi F, Hamze M. Molecular mechanisms and epidemiology of resistance in Streptococcus pneumoniae in the Middle East region. Journal Of Medical Microbiology 2017, 66: 847-858. PMID: 28650313, DOI: 10.1099/jmm.0.000503.Peer-Reviewed Original ResearchMeSH KeywordsDrug Resistance, BacterialGenes, BacterialHumansMiddle EastPneumococcal InfectionsStreptococcus pneumoniaeConceptsS. pneumoniaeStreptococcus pneumoniaeDrug-resistant S. pneumoniaeAcute otitis mediaNon-invasive infectionsEmergence of multidrugEpidemiology of resistanceS. pneumoniae strainsPrevalence of resistanceMiddle Eastern countriesMolecular mechanismsOtitis mediaMechanisms of resistanceNational surveillanceAntimicrobial stewardshipEastern countriesPneumococcal strainsPneumoniae strainsHigh mortalityNasopharyngeal cavityDecreased susceptibilityCommensal bacteriumSurveillance reportsPneumoniaeAntimicrobial resistanceMolecular detection of genes responsible for macrolide resistance among Streptococcus pneumoniae isolated in North Lebanon
Ashkar S, Osman M, Rafei R, Mallat H, Achkar M, Dabboussi F, Hamze M. Molecular detection of genes responsible for macrolide resistance among Streptococcus pneumoniae isolated in North Lebanon. Journal Of Infection And Public Health 2017, 10: 745-748. PMID: 28215918, DOI: 10.1016/j.jiph.2016.11.014.Peer-Reviewed Original ResearchConceptsMacrolide resistance genotypesS. pneumoniaeMacrolide resistanceStreptococcus pneumoniaeS. pneumoniae isolatesUse of macrolidesS. pneumoniae strainsNorth LebanonResistance genotypesCMLSB phenotypePolymerase chain reactionPneumoniae isolatesPneumoniae strainsClinical isolatesPneumoniaeChain reactionEfflux pumpsPhenotypic diagnosisCommon resistance genesMolecular detectionPrevalence, antibiotic susceptibility and characterization of antibiotic resistant genes among carbapenem-resistant Gram-negative bacilli and yeast in intestinal flora of cancer patients in North Lebanon
Christophy R, Osman M, Mallat H, Achkar M, Ziedeh A, Moukaddem W, Dabboussi F, Hamze M. Prevalence, antibiotic susceptibility and characterization of antibiotic resistant genes among carbapenem-resistant Gram-negative bacilli and yeast in intestinal flora of cancer patients in North Lebanon. Journal Of Infection And Public Health 2017, 10: 716-720. PMID: 28216021, DOI: 10.1016/j.jiph.2016.10.009.Peer-Reviewed Original ResearchConceptsCarbapenem-resistant bacteriaCancer patientsIntestinal carriageCarbapenem-resistant Gram-negative bacilliAntibiotic stewardship programsInfection control measuresDepartment of ChemotherapyGram-negative bacilliOXA-48 genePublic health concernAntimicrobial susceptibility profilesNini HospitalEndogenous infectionsMechanisms of resistanceStool samplesHigh prevalenceIntestinal floraHodge testStewardship programsPatientsAntibiotic susceptibilityNorth LebanonChemotherapySusceptibility profilesHealth concern
2016
Alarming increase in prevalence of Neisseria gonorrhoeae infections associated with a high level of antibiotic resistance in Tripoli, Lebanon
Hamze M, Osman M, Achkar M, Mallat H, Dabboussi F. Alarming increase in prevalence of Neisseria gonorrhoeae infections associated with a high level of antibiotic resistance in Tripoli, Lebanon. International Journal Of Antimicrobial Agents 2016, 48: 576-577. PMID: 27667565, DOI: 10.1016/j.ijantimicag.2016.08.003.Peer-Reviewed Original Research