2023
Genomic analysis of Shigella isolates from Lebanon reveals marked genetic diversity and antimicrobial resistance
Yassine I, Rafei R, de la Gandara M, Osman M, Fabre L, Dabboussi F, Hamze M, Weill F. Genomic analysis of Shigella isolates from Lebanon reveals marked genetic diversity and antimicrobial resistance. Microbial Genomics 2023, 9: 001157. PMID: 38100171, PMCID: PMC10763507, DOI: 10.1099/mgen.0.001157.Peer-Reviewed Original ResearchConceptsExtended-spectrum beta-lactamase genesThird-generation cephalosporinsMultidrug resistance phenotypeBeta-lactamase genesAntimicrobial resistance determinantsPrevalent serogroupsClinical isolatesQuinolone resistanceDNA gyrase genesAntimicrobial resistanceResistance determinantsCTXResistance phenotypeAmino acid substitutionsGyrase genesIsolatesNorth LebanonFirst studyAcid substitutionsPoint mutationsGenomic analysisCephalosporinsEmergence of Extended-Spectrum Cephalosporin- and Colistin-Resistant Enterobacterales in Otherwise Healthy University Students
Osman M, Yassine I, Hamze M, Al Mir H, Tajani A, Bisha B, Cummings K, Madec J, Haenni M, Kassem I. Emergence of Extended-Spectrum Cephalosporin- and Colistin-Resistant Enterobacterales in Otherwise Healthy University Students. Microbial Drug Resistance 2023, 30: 101-107. PMID: 38011748, DOI: 10.1089/mdr.2023.0213.Peer-Reviewed Original ResearchLast-resort antibioticsHealthy university studentsExtended-spectrum cephalosporin-resistant isolatesComprehensive antimicrobial stewardship programAntimicrobial stewardship programsColistin-resistant isolatesExtended-spectrum cephalosporinsCephalosporin-resistant isolatesColistin-resistant EnterobacteralesMultidrug-resistant bacteriaMiddle-income countriesEvidence-based practiceAntimicrobial stewardshipStewardship programsOccurrence of resistanceCommunity settingsAntimicrobial resistanceClinical samplesAntibiotic resistanceFecal samplesResistance determinantsAntibioticsPublic healthMultiple factorsIsolatesThe emergence of carbapenemase-producing Enterobacterales in hospitals: a major challenge for a debilitated healthcare system in Lebanon
Daaboul D, Oueslati S, Rima M, Kassem I, Mallat H, Birer A, Girlich D, Hamze M, Dabboussi F, Osman M, Naas T. The emergence of carbapenemase-producing Enterobacterales in hospitals: a major challenge for a debilitated healthcare system in Lebanon. Frontiers In Public Health 2023, 11: 1290912. PMID: 38074718, PMCID: PMC10699444, DOI: 10.3389/fpubh.2023.1290912.Peer-Reviewed Original ResearchConceptsKirby-Bauer disk diffusionDifferent Lebanese hospitalsSelf-medication practicesDisk diffusionPublic health systemCarbapenemase-producing EnterobacteralesConventional diagnostic methodsESBL producersHospital settingAntibiotic susceptibilityLebanese hospitalsClinical settingHospitalHealth systemSusceptibility profilesAntimicrobial resistanceHealthcare systemPlasmid typesHealthcare challengesDiagnostic methodsNDMCarbapenemsAMR awarenessIsolatesLebanese community
2022
Prevalence and Molecular Mechanisms of Carbapenem Resistance among Gram-Negative Bacilli in Three Hospitals of Northern Lebanon
Rima M, Oueslati S, Dabos L, Daaboul D, Mallat H, Raad E, Achkar M, Mawlawi O, Bernabeu S, Bonnin R, Girlich D, Osman M, Hamze M, Naas T. Prevalence and Molecular Mechanisms of Carbapenem Resistance among Gram-Negative Bacilli in Three Hospitals of Northern Lebanon. Antibiotics 2022, 11: 1295. PMID: 36289953, PMCID: PMC9598570, DOI: 10.3390/antibiotics11101295.Peer-Reviewed Original ResearchCarbapenem-resistant Gram-negative bacilliCarbapenem resistanceGram-negative bacilliHealth care centersCare centerEpidemiological surveyDiscordant resultsCarba NP testHealth issuesOXA-162PCR sequencingNP testHospitalPrevalenceMolecular mechanismsNovel variantsIsolatesImmunochromatographic assayRegistration systemResistance spreadOXABacilliCarbapenemsGenes
2021
Molecular Characterization of Antimicrobial Resistance and Virulence Genes of Bacterial Pathogens from Bovine and Caprine Mastitis in Northern Lebanon
Abboud Z, Galuppo L, Tolone M, Vitale M, Puleio R, Osman M, Loria G, Hamze M. Molecular Characterization of Antimicrobial Resistance and Virulence Genes of Bacterial Pathogens from Bovine and Caprine Mastitis in Northern Lebanon. Microorganisms 2021, 9: 1148. PMID: 34071800, PMCID: PMC8228836, DOI: 10.3390/microorganisms9061148.Peer-Reviewed Original ResearchMolecular characterizationMatrix-assisted laser desorptionGram-positive bacteriaResistance genesGenesPolymerase chain reactionPhylogenetic group B1Virulence factorsVirulence genesAntimicrobial resistance genesBacterial pathogensToxin genesLaser desorptionMastitis-causing pathogensNegative bacteriaBeta-lactamase genesFlight mass spectrometryBacterial identificationBacteriaQuadruplex PCR methodSymptoms of mastitisDairy animalsPhylogenetic typingIsolatesPathogensSpecies distribution and antifungal susceptibility of Aspergillus clinical isolates in Lebanon
Osman M, Bidon B, Abboud C, Zakaria A, Hamze B, Achcar M, Mallat H, Dannaoui E, Dabboussi F, Papon N, Bouchara J, Hamze M. Species distribution and antifungal susceptibility of Aspergillus clinical isolates in Lebanon. Future Microbiology 2021, 16: 13-26. PMID: 33438473, DOI: 10.2217/fmb-2020-0141.Peer-Reviewed Original ResearchConceptsAntifungal susceptibilityAspergillus clinical isolatesAntifungal susceptibility patternsReverse transcription-quantitative PCRNational Reference CenterOverall drug resistanceTranscription-quantitative PCRInvasive mycosesSusceptibility patternsHealth strategiesEUCAST recommendationsReference centerClinical isolatesAmphotericin BDrug resistanceResistant isolatesMajor causeGene mutationsExpression levelsIsolatesEpidemiologySusceptibilityMycoses
2019
Prevalence of Staphylococcus aureus methicillin-sensitive and methicillin-resistant nasal carriage in food handlers in Lebanon: a potential source of transmission of virulent strains in the community
Osman M, Kamal-Dine K, Omari K, Rafei R, Dabboussi F, Hamze M. Prevalence of Staphylococcus aureus methicillin-sensitive and methicillin-resistant nasal carriage in food handlers in Lebanon: a potential source of transmission of virulent strains in the community. Access Microbiology 2019, 1: e000043. PMID: 32974533, PMCID: PMC7470302, DOI: 10.1099/acmi.0.000043.Peer-Reviewed Original ResearchNasal carriageFood handlersCross-sectional study designNasal swab specimensAntibiotic susceptibility patternEnterotoxin genesGenetic virulence determinantsLevel of MRSACarriage prevalenceNasal colonizationVirulence determinant genesSwab specimensSusceptibility patternsStudy designMicrobiological investigationsPrevalenceFood poisoningVirulence determinantsCarriageVirulent strainStaphylococcus aureusConventional cultureMRSAIsolatesLast reportMolecular epidemiology of Campylobacter isolates from broiler slaughterhouses in Tripoli, North of Lebanon
Rafei R, Al Kassaa I, Osman M, Dabboussi F, Hamze M. Molecular epidemiology of Campylobacter isolates from broiler slaughterhouses in Tripoli, North of Lebanon. British Poultry Science 2019, 60: 675-682. PMID: 31321996, DOI: 10.1080/00071668.2019.1645945.Peer-Reviewed Original ResearchMeSH KeywordsAbattoirsAnimalsAnti-Bacterial AgentsCampylobacterCampylobacter InfectionsCecumChickensDrug Resistance, BacterialElectrophoresis, Gel, Pulsed-FieldFlagellinFoodborne DiseasesGenetic VariationHumansLebanonPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthPoultry DiseasesPrevalenceSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationConceptsAntibiotic resistance profilesErtapenem resistanceMolecular epidemiologyReal burdenResistance profilesAntibiotic resistanceRestriction fragment length polymorphismNorth of LebanonFragment length polymorphismEpidemiologyField gel electrophoresisCampylobacterLength polymorphismBroiler slaughterhousesBroilersIsolatesSlaughterhouseGentamicinAmoxicillinErythromycin