2023
Identification of genes influencing the evolution of Escherichia coli ST372 in dogs and humans
Elankumuran P, Browning G, Marenda M, Kidsley A, Osman M, Haenni M, Johnson J, Trott D, Reid C, Djordjevic S. Identification of genes influencing the evolution of Escherichia coli ST372 in dogs and humans. Microbial Genomics 2023, 9: mgen000930. PMID: 36752777, PMCID: PMC9997745, DOI: 10.1099/mgen.0.000930.Peer-Reviewed Original ResearchConceptsPopulation structureIdentification of genesWhole genome sequencesPropanediol utilization (pdu) operonGenomic traitsAccessory genomeCanine sourcesBioinformatics analysisUtilization operonPhylogenetic clustersGlobal collectionDistinct populationsVirulence genesOperonGenesDominant hostSequence typesSequenceExtraintestinal infectionsPhylogenyCladeGenomeLineagesHumansTraits
2022
Prevalence and Molecular Mechanisms of Carbapenem Resistance among Gram-Negative Bacilli in Three Hospitals of Northern Lebanon
Rima M, Oueslati S, Dabos L, Daaboul D, Mallat H, Raad E, Achkar M, Mawlawi O, Bernabeu S, Bonnin R, Girlich D, Osman M, Hamze M, Naas T. Prevalence and Molecular Mechanisms of Carbapenem Resistance among Gram-Negative Bacilli in Three Hospitals of Northern Lebanon. Antibiotics 2022, 11: 1295. PMID: 36289953, PMCID: PMC9598570, DOI: 10.3390/antibiotics11101295.Peer-Reviewed Original ResearchCarbapenem-resistant Gram-negative bacilliCarbapenem resistanceGram-negative bacilliHealth care centersCare centerEpidemiological surveyDiscordant resultsCarba NP testHealth issuesOXA-162PCR sequencingNP testHospitalPrevalenceMolecular mechanismsNovel variantsIsolatesImmunochromatographic assayRegistration systemResistance spreadOXABacilliCarbapenemsGenes
2021
Cl415, a carbapenem-resistant Acinetobacter baumannii isolate containing four AbaR4 and a new variant of AbGRI2, represents a novel global clone 2 strain
Liepa R, Mann R, Osman M, Hamze M, Gunawan C, Hamidian M. Cl415, a carbapenem-resistant Acinetobacter baumannii isolate containing four AbaR4 and a new variant of AbGRI2, represents a novel global clone 2 strain. Journal Of Antimicrobial Chemotherapy 2021, 77: 345-350. PMID: 34741594, PMCID: PMC8809195, DOI: 10.1093/jac/dkab399.Peer-Reviewed Original ResearchConceptsWhole genome sequencesWide geographical distributionGene complementNovel variantsPhylogenetic analysisGenomic analysisNew lineageComplete genomeOxford NanoporeIllumina MiSeqGenetic contextResistance genesGenesAntibiotic resistance determinantsResistance phenotypeGeographical distributionCopiesAntibiotic resistance phenotypesResistance islandsGlobal distributionResistance determinantsComM geneAntibiotic resistanceSubcladesVariantsMolecular Characterization of Antimicrobial Resistance and Virulence Genes of Bacterial Pathogens from Bovine and Caprine Mastitis in Northern Lebanon
Abboud Z, Galuppo L, Tolone M, Vitale M, Puleio R, Osman M, Loria G, Hamze M. Molecular Characterization of Antimicrobial Resistance and Virulence Genes of Bacterial Pathogens from Bovine and Caprine Mastitis in Northern Lebanon. Microorganisms 2021, 9: 1148. PMID: 34071800, PMCID: PMC8228836, DOI: 10.3390/microorganisms9061148.Peer-Reviewed Original ResearchMolecular characterizationMatrix-assisted laser desorptionGram-positive bacteriaResistance genesGenesPolymerase chain reactionPhylogenetic group B1Virulence factorsVirulence genesAntimicrobial resistance genesBacterial pathogensToxin genesLaser desorptionMastitis-causing pathogensNegative bacteriaBeta-lactamase genesFlight mass spectrometryBacterial identificationBacteriaQuadruplex PCR methodSymptoms of mastitisDairy animalsPhylogenetic typingIsolatesPathogens