2003
Localized Cdc42 Activation, Detected Using a Novel Assay, Mediates Microtubule Organizing Center Positioning in Endothelial Cells in Response to Fluid Shear Stress*
Tzima E, Kiosses WB, del Pozo MA, Schwartz MA. Localized Cdc42 Activation, Detected Using a Novel Assay, Mediates Microtubule Organizing Center Positioning in Endothelial Cells in Response to Fluid Shear Stress*. Journal Of Biological Chemistry 2003, 278: 31020-31023. PMID: 12754216, DOI: 10.1074/jbc.m301179200.Peer-Reviewed Original ResearchConceptsMicrotubule organizing centerCdc42 activityCdc42 activationFluid shear stressSmall GTPase Cdc42Protein kinase CzetaGTPase Cdc42Early embryosEndothelial cellsIntegrin dynamicsOrganizing centerCdc42Extracellular matrixLocalized activationFluorescence energy transferMTOC localizationVascular endothelial cellsSingle cellsNovel assayCellsActivationPar6CzetaGolgiEmbryos
1982
A new radioactive cross-linking reagent for studying the interactions of proteins.
Schwartz M, Das O, Hynes R. A new radioactive cross-linking reagent for studying the interactions of proteins. Journal Of Biological Chemistry 1982, 257: 2343-2349. PMID: 7061425, DOI: 10.1016/s0021-9258(18)34928-7.Peer-Reviewed Original ResearchMeSH KeywordsAzidesCross-Linking ReagentsFibronectinsGelatinIndicators and ReagentsMethodsPhotolysisProteinsSepharoseStructure-Activity RelationshipSuccinimidesConceptsCross-linking reagentInteraction of proteinsChemical cross-linking reagentsBinding of gelatinCovalent derivativesChemical behaviorSecond moleculeNeighboring macromoleculesN-succinimidyl propionateReagentsHigh yieldsBiological systemsNitro-4DerivativesBiological conditionsPhotolysisMacromoleculesMoleculesInteractionGelatinSituSurfaceYield