High-resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry
Lechene C, Hillion F, McMahon G, Benson D, Kleinfeld A, Kampf J, Distel D, Luyten Y, Bonventre J, Hentschel D, Park K, Ito S, Schwartz M, Benichou G, Slodzian G. High-resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry. BMC Biology 2006, 5: 20. PMID: 17010211, PMCID: PMC1781526, DOI: 10.1186/jbiol42.Peer-Reviewed Original ResearchConceptsMulti-isotope imaging mass spectrometryMammalian cultured cellsFatty acid transportAdipocyte lipid dropletsHigh-resolution quantitative imagingSubcellular compartmentsNitrogen fixationMass spectrometryImaging mass spectrometryLevel of enrichmentBacterial cellsProtein renewalCultured endothelial cellsCultured cellsLipid dropletsKidney cellsStable isotopesUridine incorporationEndothelial cellsCellsQuantitative image analysis softwareStable isotope mass spectrometryIsotopic labelsIsotope ratiosRNA