2000
Activating Phosphorylation of the Saccharomyces cerevisiae Cyclin-dependent Kinase, Cdc28p, Precedes Cyclin Binding
Ross K, Kaldis P, Solomon M. Activating Phosphorylation of the Saccharomyces cerevisiae Cyclin-dependent Kinase, Cdc28p, Precedes Cyclin Binding. Molecular Biology Of The Cell 2000, 11: 1597-1609. PMID: 10793138, PMCID: PMC14870, DOI: 10.1091/mbc.11.5.1597.Peer-Reviewed Original ResearchMeSH KeywordsAntibody SpecificityCDC28 Protein Kinase, S cerevisiaeCell CycleCyclin ACyclin BCyclin-Dependent Kinase-Activating KinaseCyclin-Dependent KinasesCyclinsEnzyme ActivationEpitopesMutationPhosphorylationProtein Serine-Threonine KinasesRecombinant ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsThreonineConceptsCyclin bindingCyclin-dependent kinasesEukaryotic cell cycle progressionConserved threonine residueAddition of cyclinCell cycle progressionHigher eukaryotesThreonine residuesCdc28pCDK activationProtein kinaseRegulatory mechanismsCycle progressionCell cycleCyclinKinasePhosphorylationCak1pActivation pathwayCDKBindingEukaryotesActivationMutantsThreonine
1999
Activating Phosphorylation of the Kin28p Subunit of Yeast TFIIH by Cak1p
Kimmelman J, Kaldis P, Hengartner C, Laff G, Koh S, Young R, Solomon M. Activating Phosphorylation of the Kin28p Subunit of Yeast TFIIH by Cak1p. Molecular And Cellular Biology 1999, 19: 4774-4787. PMID: 10373527, PMCID: PMC84276, DOI: 10.1128/mcb.19.7.4774.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CycleCyclin-Dependent Kinase-Activating KinaseCyclin-Dependent KinasesEnzyme ActivationPhosphorylationPoint MutationProtein Serine-Threonine KinasesRabbitsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTATA-Binding Protein Associated FactorsThreonineTranscription Factor TFIIDTranscription Factor TFIIHTranscription FactorsTranscription Factors, TFIIConceptsKinase activityGeneral transcription factor TFIIHTranscription factor TFIIHCTD kinase activityRNA polymerase IICell cycle CDKsCyclin-dependent kinasesPrevious biochemical observationsYeast TFIIHPolymerase IIActivating PhosphorylationLarge subunitCak1pCatalytic subunitKinase subunitTerminal domainTFIIHConditional alleleCell cyclePhosphorylationCAKSubunitsCDKMO15Kinase
1998
Localization and regulation of the cdk-activating kinase (Cak1p) from budding yeast
Kaldis P, Pitluk Z, Bany I, Enke D, Wagner M, Winter E, Solomon M. Localization and regulation of the cdk-activating kinase (Cak1p) from budding yeast. Journal Of Cell Science 1998, 111: 3585-3596. PMID: 9819350, DOI: 10.1242/jcs.111.24.3585.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceCDC28 Protein Kinase, S cerevisiaeCyclin-Dependent Kinase-Activating KinaseCyclin-Dependent KinasesCytoplasmFluorescent Antibody TechniqueGene Expression Regulation, FungalIsoelectric FocusingMeiosisMolecular Sequence DataMutagenesis, Site-DirectedPhosphorylationProtein Serine-Threonine KinasesSaccharomyces cerevisiaeSubcellular FractionsConceptsBiochemical subcellular fractionationEukaryotic cell cyclePost-translational levelCyclin-dependent kinasesMajor CdkThreonine 169Two-dimensional isoelectric focusingCak1pPosttranslational modificationsStable proteinSubcellular fractionationMonomeric enzymeCell cycleYeastCDKKinaseCAKFurther characterizationPhosphorylationRegulationIsoelectric focusingPotential sitesCdc28pCellsMeiosisCyclin-Stimulated Binding of Cks Proteins to Cyclin-Dependent Kinases
Egan E, Solomon M. Cyclin-Stimulated Binding of Cks Proteins to Cyclin-Dependent Kinases. Molecular And Cellular Biology 1998, 18: 3659-3667. PMID: 9632748, PMCID: PMC108948, DOI: 10.1128/mcb.18.7.3659.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsCDC2 Protein KinaseCDC2-CDC28 KinasesCell Cycle ProteinsCyclin ACyclin BCyclin-Dependent Kinase 2Cyclin-Dependent Kinase-Activating KinaseCyclin-Dependent KinasesEnzyme ActivationPhosphorylationProtein BindingProtein KinasesProtein Serine-Threonine KinasesRecombinant Fusion ProteinsThreonineXenopusXenopus Proteins
1996
A Predictive Scale for Evaluating Cyclin-dependent Kinase Substrates A COMPARISON OF p34 cdc2 AND p33 cdk2 *
Holmes J, Solomon M. A Predictive Scale for Evaluating Cyclin-dependent Kinase Substrates A COMPARISON OF p34 cdc2 AND p33 cdk2 *. Journal Of Biological Chemistry 1996, 271: 25240-25246. PMID: 8810285, DOI: 10.1074/jbc.271.41.25240.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBinding SitesCDC2 Protein KinaseCDC2-CDC28 KinasesCell CycleCell LineConsensus SequenceCyclin-Dependent Kinase 2Cyclin-Dependent KinasesCyclinsGlutathione TransferaseHumansMutagenesis, Site-DirectedOligopeptidesPolymerase Chain ReactionProtein Serine-Threonine KinasesRecombinant Fusion ProteinsSpodopteraSubstrate SpecificityTransfectionXenopusXenopus ProteinsConceptsSubstrate specificityDistinct substrate specificitiesPrimary sequence determinantsCyclin-dependent kinasesCDK substratesCyclin subunitProtein phosphorylationProtein kinaseCDK familyP34 cdc2Consensus sequenceSequence determinantsCell cyclePhosphorylation efficiencyKinasePeptide fusionsPhosphorylation potentialSignificant oversimplificationCdc2PhosphorylationSpecificitySubunitsCDK2SpeciesTrue specificity
1995
KIN28 Encodes a C-Terminal Domain Kinase That Controls mRNA Transcription in Saccharomyces cerevisiae but Lacks Cyclin-Dependent Kinase-Activating Kinase (CAK) Activity
Cismowski M, Laff G, Solomon M, Reed S. KIN28 Encodes a C-Terminal Domain Kinase That Controls mRNA Transcription in Saccharomyces cerevisiae but Lacks Cyclin-Dependent Kinase-Activating Kinase (CAK) Activity. Molecular And Cellular Biology 1995, 15: 2983-2992. PMID: 7760796, PMCID: PMC230529, DOI: 10.1128/mcb.15.6.2983.Peer-Reviewed Original ResearchConceptsC-terminal domainKinase activityCTD kinaseMRNA transcriptionCritical threonine residueCyclin-dependent kinase familyCTD kinase activityRNA polymerase IICell cycle CDKsExtensive sequence identityCyclin-dependent kinasesThermosensitive alleleCTD phosphorylationPolymerase IIThreonine residuesKinase familyPositive regulatorKin28Phosphorylation stateS. cerevisiaeSequence identityCell cycleTranscriptionHuman cellsCDK
1994
Inactivation of a Cdk2 inhibitor during interleukin 2-induced proliferation of human T lymphocytes.
Firpo E, Koff A, Solomon M, Roberts J. Inactivation of a Cdk2 inhibitor during interleukin 2-induced proliferation of human T lymphocytes. Molecular And Cellular Biology 1994, 14: 4889-4901. PMID: 7516474, PMCID: PMC358861, DOI: 10.1128/mcb.14.7.4889.Peer-Reviewed Original ResearchMeSH KeywordsAdultCDC2-CDC28 KinasesCell CycleCells, CulturedCyclin-Dependent Kinase 2Cyclin-Dependent Kinase Inhibitor p21Cyclin-Dependent KinasesCyclinsDNAEnzyme ActivationFlow CytometryHumansInterleukin-2KineticsLymphocyte ActivationProtein Kinase InhibitorsProtein KinasesProtein Serine-Threonine KinasesReceptors, Antigen, T-CellReceptors, Interleukin-2Recombinant ProteinsRNASignal TransductionTime FactorsT-LymphocytesConceptsCyclin-dependent kinasesMitogenic signalsCyclin-cdk2 complexesCDK2 inhibitorsNegative growth signalsG1 cyclin-CDK complexesCell cycle commitmentCyclin-CDK complexesCyclin-Cdk inhibitorT cell antigen receptorCell proliferationAntigen receptor stimulationCell cycle proteinsInhibitors of CDK2Mitogenic growth factorsGrowth signalsSimilar proteinsBiochemical pathwaysCell cycleCDK inhibitorsCdk2 activationCycle proteinsRestriction pointS phaseCommon targetInactivation of a Cdk2 Inhibitor during Interleukin 2-Induced Proliferation of Human T Lymphocytes
Firpo E, Koff A, Solomon M, Roberts J. Inactivation of a Cdk2 Inhibitor during Interleukin 2-Induced Proliferation of Human T Lymphocytes. Molecular And Cellular Biology 1994, 14: 4889-4901. DOI: 10.1128/mcb.14.7.4889-4901.1994.Peer-Reviewed Original ResearchCyclin-dependent kinasesMitogenic signalsCyclin-cdk2 complexesCDK2 inhibitorsNegative growth signalsG1 cyclin-CDK complexesCell cycle commitmentCyclin-CDK complexesCyclin-Cdk inhibitorT cell antigen receptorCell proliferationAntigen receptor stimulationCell cycle proteinsInhibitors of CDK2Mitogenic growth factorsGrowth signalsSimilar proteinsBiochemical pathwaysCell cycleCDK inhibitorsCdk2 activationCycle proteinsRestriction pointS phaseCommon targetInactivation of a Cdk2 Inhibitor during Interleukin 2-Induced Proliferation of Human T Lymphocytes
Firpo E, Koff A, Solomon M, Roberts J. Inactivation of a Cdk2 Inhibitor during Interleukin 2-Induced Proliferation of Human T Lymphocytes. Molecular And Cellular Biology 1994, 14: 4889-4901. DOI: 10.1128/mcb.14.7.4889-4901.1994.Peer-Reviewed Original ResearchCyclin-dependent kinasesMitogenic signalingPromoter activityActivation of cell cycle proteinsCyclin-CDK inhibitorCell cycle commitmentCyclin-CDK complexesCyclin-cdk2 complexesCell cycle proteinsG1 cyclin-CDK complexesCyclin-dependent kinase inhibitorNegative growth signalsT cell antigen receptorRegulating cell proliferationCell proliferationCDK2 inhibitorsAntigen receptor stimulationInhibitor of CDK2CDK2 activityCell cycleCycle proteinsRestriction pointGrowth signalsAffinity chromatographyMitogenic growth factorsp27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest.
Polyak K, Kato J, Solomon M, Sherr C, Massague J, Roberts J, Koff A. p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest. Genes & Development 1994, 8: 9-22. PMID: 8288131, DOI: 10.1101/gad.8.1.9.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCDC2-CDC28 KinasesCell CommunicationCell CycleCell LineCyclin-Dependent Kinase 2Cyclin-Dependent Kinase-Activating KinaseCyclin-Dependent KinasesCyclinsMinkProtein Kinase InhibitorsProtein KinasesProtein Serine-Threonine KinasesProteinsProtein-Tyrosine KinasesSignal TransductionTransforming Growth Factor betaConceptsCdk2 complexesCyclin ECyclin D2-Cdk4 complexesCyclin-Cdk inhibitorCell-cell contactGrowth inhibitory signalsCell cycle arrestCatalytic subunitG1 progressionG1 cyclinsCell cycleCdk2 activationCycle arrestCDK2 inhibitionContact inhibitionCyclin E levelsActive complexAffinity chromatographyEpithelial cellsCDK2Inhibitory signalsCellsP27ComplexesActivation