2005
Myosin-1a Is Critical for Normal Brush Border Structure and Composition
Tyska M, Mackey A, Huang J, Copeland N, Jenkins N, Mooseker M. Myosin-1a Is Critical for Normal Brush Border Structure and Composition. Molecular Biology Of The Cell 2005, 16: 2443-2457. PMID: 15758024, PMCID: PMC1087248, DOI: 10.1091/mbc.e04-12-1116.Peer-Reviewed Original ResearchConceptsMyosin-1aWhole animal phenotypesWhole animal levelIntermediate filament proteinsEctopic recruitmentFunctional redundancyAnimal phenotypesBrush borderMyosin 1cOvert phenotypeBrush border structureFilament proteinsMembrane componentsCellular levelVertebrate myosinsPhenotypeSigns of stressAnimal levelKnockout miceSignificant perturbationsEnterocytesMultifunctional componentsGenesDistinct changesProtein
2004
A role for myosin-1A in the localization of a brush border disaccharidase
Tyska M, Mooseker M. A role for myosin-1A in the localization of a brush border disaccharidase. Journal Of Cell Biology 2004, 165: 395-405. PMID: 15138292, PMCID: PMC2172191, DOI: 10.1083/jcb.200310031.Peer-Reviewed Original Research
2002
MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells
Tyska M, Mooseker M. MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells. Biophysical Journal 2002, 82: 1869-1883. PMID: 11916846, PMCID: PMC1301984, DOI: 10.1016/s0006-3495(02)75537-9.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBiophysical PhenomenaBiophysicsCloning, MolecularCytoskeletonElectrophoresis, Polyacrylamide GelGreen Fluorescent ProteinsHumansKidneyKineticsLLC-PK1 CellsLuminescent ProteinsMicroscopy, ConfocalMicroscopy, FluorescenceMicrovilliMyosin Type IPrecipitin TestsProtein Structure, TertiaryRecombinant Fusion ProteinsSubcellular FractionsSwineTime FactorsTransfectionConceptsTail domainLLC-PK1Actin core bundleKidney epithelial cell lineApical targetingActin dynamicsBrush borderActin turnoverGFP-actinBB populationsEpithelial cell lineActin domainsFluorescence recoveryCl4 cellsRapid turnoverApical surfaceMotor domainCore bundleATP depletionCell linesActinTurnoverCellsDomain
2001
Myosin-VIIb, a Novel Unconventional Myosin, Is a Constituent of Microvilli in Transporting Epithelia
Chen Z, Hasson T, Zhang D, Schwender B, Derfler B, Mooseker M, Corey D. Myosin-VIIb, a Novel Unconventional Myosin, Is a Constituent of Microvilli in Transporting Epithelia. Genomics 2001, 72: 285-296. PMID: 11401444, DOI: 10.1006/geno.2000.6456.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsBase SequenceBiological TransportBlotting, NorthernBlotting, WesternChromosome MappingCloning, MolecularDNA, ComplementaryEpitheliumExonsFemaleGenesImmunohistochemistryIntestinesIntronsKidneyMiceMice, Inbred BALB CMicrovilliMolecular Sequence DataMyosinsPhylogenyProtein IsoformsRNA, MessengerSequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidTissue Distribution
1999
Cloning and characterization of mouse brush border myosin‐I in adult and embryonic intestine
Skowron J, Mooseker M. Cloning and characterization of mouse brush border myosin‐I in adult and embryonic intestine. Journal Of Experimental Zoology 1999, 283: 242-257. PMID: 9933937, DOI: 10.1002/(sici)1097-010x(19990215)283:3<242::aid-jez3>3.0.co;2-f.Peer-Reviewed Original ResearchConceptsBrush border myosinMicrovillar actin bundlesEmbryonic intestineSubcellular localization profileBasolateral membrane domainsGenomic clonesCalmodulin light chainsVertebrate speciesMembrane domainsActin corePlasma membraneKnockout strategiesActin bundlesImmunolocalization studiesIntestinal epithelial cellsGolgi apparatusNorthern analysisPrimary structureNorthern blotSitu localizationLocalization profilesRNA expressionEmbryosDirect targetingEmbryogenesis
1998
Human brush border myosin‐I and myosin‐Ic expression in human intestine and Caco‐2BBe cells
Skowron J, Bement W, Mooseker M. Human brush border myosin‐I and myosin‐Ic expression in human intestine and Caco‐2BBe cells. Cytoskeleton 1998, 41: 308-324. PMID: 9858156, DOI: 10.1002/(sici)1097-0169(1998)41:4<308::aid-cm4>3.0.co;2-j.Peer-Reviewed Original Research
1995
Characterization of Myosin-IA and Myosin-IB, Two Unconventional Myosins Associated with theDrosophilaBrush Border Cytoskeleton
Morgan N, Heintzelman M, Mooseker M. Characterization of Myosin-IA and Myosin-IB, Two Unconventional Myosins Associated with theDrosophilaBrush Border Cytoskeleton. Developmental Biology 1995, 172: 51-71. PMID: 7589814, DOI: 10.1006/dbio.1995.0005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCloning, MolecularCytoskeletonDrosophila melanogasterElectrophoresis, Polyacrylamide GelEmbryo, NonmammalianFemaleGene Expression RegulationImmunoblottingImmunohistochemistryLarvaMicroscopy, ElectronMicroscopy, ImmunoelectronMicrovilliMyosinsOogenesisOvumPupaRecombinant ProteinsConceptsMyosin IBAdult gutATP-dependent extractionMyosin IASomatic follicle cellsExogenous F-actinBrush border cytoskeletonNovel unconventional myosinDrosophila melanogasterMicrovillus assemblyEgg chambersApical domainMicrovillar componentsMicrovillar domainApical microvillar domainBasolateral domainExpression patternsUnconventional myosinF-actinSubcellular distributionFollicle cellsFunctional myosinDifferentiated enterocytesBiochemical propertiesBrush border
1994
Multiple unconventional myosin domains of the intestinal brush border cytoskeleton
Heintzelman M, Hasson T, Mooseker M. Multiple unconventional myosin domains of the intestinal brush border cytoskeleton. Journal Of Cell Science 1994, 107: 3535-3543. PMID: 7706404, DOI: 10.1242/jcs.107.12.3535.Peer-Reviewed Original ResearchDifferential regulation of skeletal muscle myosin‐II and brush border myosin‐I enzymology and mechanochemistry by bacterially produced tropomyosin isoforms
Fanning A, Wolenski J, Mooseker M, Izant J. Differential regulation of skeletal muscle myosin‐II and brush border myosin‐I enzymology and mechanochemistry by bacterially produced tropomyosin isoforms. Cytoskeleton 1994, 29: 29-45. PMID: 7820856, DOI: 10.1002/cm.970290104.Peer-Reviewed Original ResearchMeSH KeywordsActinsAmino Acid SequenceAnimalsCa(2+) Mg(2+)-ATPaseCarrier ProteinsCell MovementChick EmbryoDNA, ComplementaryEscherichia coliIntestinal MucosaMicrofilament ProteinsMicrovilliMolecular Sequence DataMolecular WeightMuscle ProteinsMyosinsProtein BindingRecombinant Fusion ProteinsRecombinant ProteinsSequence HomologySpecies SpecificityTropomyosinVertebratesXenopusConceptsAlpha-tropomyosinMuscle myosin IIMyosin enzymologyMyosin isoformsTwo- toStriated MuscleMgATPase activityDifferential regulationBrush border myosinDetectable effectIsoformsLow affinityChimeric tropomyosinsMuscle tropomyosinMuscle alpha-tropomyosinSkeletal muscle myosin IIActinTropomyosin isoformsMuscle myosinF-actin
1993
In vitro motilities of the unconventional myosins, brush border myosin‐I, and chick brain myosin‐V exhibit assay‐dependent differences in velocity
Wolenski J, Cheney R, Forscher P, Mooseker M. In vitro motilities of the unconventional myosins, brush border myosin‐I, and chick brain myosin‐V exhibit assay‐dependent differences in velocity. Journal Of Experimental Zoology 1993, 267: 33-39. PMID: 8376949, DOI: 10.1002/jez.1402670106.Peer-Reviewed Original ResearchCalcium-calmodulin and regulation of brush border myosin-I MgATPase and mechanochemistry
Wolenski J, Hayden S, Forscher P, Mooseker. Calcium-calmodulin and regulation of brush border myosin-I MgATPase and mechanochemistry. Journal Of Cell Biology 1993, 122: 613-621. PMID: 8335688, PMCID: PMC2119657, DOI: 10.1083/jcb.122.3.613.Peer-Reviewed Original ResearchAn in vitro model for the analysis of intestinal brush border assembly I. Ultrastructural analysis of cell contact-induced brush border assembly in Caco-2BBe cells
Peterson M, Mooseker M. An in vitro model for the analysis of intestinal brush border assembly I. Ultrastructural analysis of cell contact-induced brush border assembly in Caco-2BBe cells. Journal Of Cell Science 1993, 105: 445-460. PMID: 8408276, DOI: 10.1242/jcs.105.2.445.Peer-Reviewed Original ResearchAn in vitro model for the analysis of intestinal brush border assembly. II. Changes in expression and localization of brush border proteins during cell contact-induced brush border assembly in Caco-2BBe cells.
Peterson M, Bement W, Mooseker M. An in vitro model for the analysis of intestinal brush border assembly. II. Changes in expression and localization of brush border proteins during cell contact-induced brush border assembly in Caco-2BBe cells. Journal Of Cell Science 1993, 105 ( Pt 2): 461-72. PMID: 8408277, DOI: 10.1242/jcs.105.2.461.Peer-Reviewed Original ResearchActinsAdenocarcinomaAmino Acid SequenceAnimalsBiomarkersBirdsCarrier ProteinsCattleCell CommunicationCell PolarityClone CellsColonic NeoplasmsCytoskeletonGene Expression Regulation, NeoplasticHumansIntestinal MucosaMicrofilament ProteinsMicrovilliMolecular Sequence DataMyosinsNeoplasm ProteinsOligo-1,6-GlucosidaseSequence AlignmentSequence Homology, Amino AcidSpecies SpecificitySucraseTumor Cells, Cultured
1992
Localization of capping protein in chicken epithelial cells by immunofluorescence and biochemical fractionation.
Schafer D, Mooseker M, Cooper J. Localization of capping protein in chicken epithelial cells by immunofluorescence and biochemical fractionation. Journal Of Cell Biology 1992, 118: 335-346. PMID: 1629237, PMCID: PMC2290044, DOI: 10.1083/jcb.118.2.335.Peer-Reviewed Original ResearchMeSH KeywordsActin Depolymerizing FactorsActinsAnimalsAntibodiesCells, CulturedChick EmbryoChickensCochleaDestrinElectrophoresis, Polyacrylamide GelEpithelial CellsEpitheliumFluorescent Antibody TechniqueIntestinesKidneyMacromolecular SubstancesMicrofilament ProteinsMicrovilliMusclesPigment Epithelium of EyeSubcellular FractionsConceptsIntestinal epithelial cellsNuclei of cellsBiochemical fractionationJunctional complexesActin filamentsCell-cell junctional complexesSingle intestinal epithelial cellsBarbed endsEpithelial cellsSensory epitheliumCell-cell contactIntact intestinal epithelial cellsAffinity-purified polyclonal antibodiesProtein bindsCapping proteinIntestinal epitheliumZonula adherensCell junctionsChick embryo kidney cellsPattern coincidentEmbryo kidney cellsProteinConfocal microscopyKidney cellsIsolated intestinal epithelial cellsCharacterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2
Peterson M, Mooseker M. Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2. Journal Of Cell Science 1992, 102: 581-600. PMID: 1506435, DOI: 10.1242/jcs.102.3.581.Peer-Reviewed Original ResearchConceptsMyosin IBB myosin IC2BBe cellsATP-dependent associationBrush border cytoskeletonApical brush borderHeavy chainDomain-specific monoclonal antibodiesBrush borderFilter-grown monolayersCaco-2Cell line Caco-2Human colonic epithelial cellsHuman intestinal cell lineMyosin I.Myosin IIIntestinal cell linePhysical associationLaser scanning confocal microscopyProtein villinCytoskeletonCell culture modelCell typesPattern of distributionVivo structureThe molecular architecture of an insect midgut brush border cytoskeleton.
Bonfanti P, Colombo A, Heintzelman M, Mooseker M, Camatini M. The molecular architecture of an insect midgut brush border cytoskeleton. European Journal Of Cell Biology 1992, 57: 298-307. PMID: 1511705.Peer-Reviewed Original ResearchConceptsTwo-dimensional gel analysisMolecular architectureMultiple isoelectric variantsBrush border cytoskeletonLepidopteran larvaeBB myosin IVertebrate intestineBrush borderMyosin IOrganelle exclusionHeavy meromyosin decorationCytoskeletal apparatusMyosin IINonmuscle cellsActin filamentsCytoskeletonIsoelectric variantsGel analysisInstar larvaeMajor isoformsStructural organizationRhodamine phalloidinCore proteinIntestinal brush borderMidgutUnconventional myosins
Cheney R, Mooseker M. Unconventional myosins. Current Opinion In Cell Biology 1992, 4: 27-35. PMID: 1558751, DOI: 10.1016/0955-0674(92)90055-h.Peer-Reviewed Original Research6 Assembly of the Intestinal Brush Border Cytoskeleton
Heintzelman M, Mooseker M. 6 Assembly of the Intestinal Brush Border Cytoskeleton. Current Topics In Developmental Biology 1992, 26: 93-122. PMID: 1563281, DOI: 10.1016/s0070-2153(08)60442-1.Peer-Reviewed Original Research
1990
Binding of brush border myosin I to phospholipid vesicles.
Hayden S, Wolenski J, Mooseker M. Binding of brush border myosin I to phospholipid vesicles. Journal Of Cell Biology 1990, 111: 443-451. PMID: 2143194, PMCID: PMC2116197, DOI: 10.1083/jcb.111.2.443.Peer-Reviewed Original ResearchConceptsBB myosin IMyosin IBrush border myosin IMyosin I heavy chainMembrane-binding domainActin filament corePhospholipid vesiclesATP-sensitive mannerPlasma membraneIntestinal epithelial cellsF-actinMembrane interactionsPhosphatidylglycerol vesiclesFree proteinCOOH-terminalAnionic phospholipidsImmunoblot analysisVesiclesEpithelial cellsImmunoblot stainingM. SimilarHeavy chainActinStructural informationNeutral phospholipidsStructural and compositional analysis of early stages in microvillus assembly in the enterocyte of the chick embryo
Heintzelman M, Mooseker M. Structural and compositional analysis of early stages in microvillus assembly in the enterocyte of the chick embryo. Differentiation 1990, 43: 175-182. PMID: 2387484, DOI: 10.1111/j.1432-0436.1990.tb00444.x.Peer-Reviewed Original Research