2008
Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid–protein complex that contains mRNAs and subunits of the RNA-processing body
Chang W, Zaarour RF, Reck-Peterson S, Rinn J, Singer RH, Snyder M, Novick P, Mooseker MS. Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid–protein complex that contains mRNAs and subunits of the RNA-processing body. RNA 2008, 14: 491-502. PMID: 18218704, PMCID: PMC2248268, DOI: 10.1261/rna.665008.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdenosine TriphosphatasesBase SequenceDNA PrimersMacromolecular SubstancesMyosin Heavy ChainsMyosin Type VOligonucleotide Array Sequence AnalysisOrganellesPolyribosomesRibonucleoproteinsRNA Processing, Post-TranscriptionalRNA, FungalRNA, MessengerSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSecretory VesiclesVacuolesConceptsRNA processing bodiesClass V myosinsP-bodiesRelease of mRNAProcessing bodiesOrganelle traffickingSpindle orientationMotor mutantsMyo2-66Ribosomal subunitMyo2pProtein subunitsPartial colocalizationMicroarray analysisSubunitsSedimentation analysisYeastMRNAComplexesMyosinMutantsPolysomesTraffickingRNAColocalization
2005
Cell Polarity Protein Spa2P Associates With Proteins Involved In Actin Function In Saccharomyces Cerevisiae
Shih J, Reck-Peterson S, Newitt R, Mooseker M, Aebersold R, Herskowitz I. Cell Polarity Protein Spa2P Associates With Proteins Involved In Actin Function In Saccharomyces Cerevisiae. Molecular Biology Of The Cell 2005, 16: 4595-4608. PMID: 16030260, PMCID: PMC1237067, DOI: 10.1091/mbc.e05-02-0108.Peer-Reviewed Original ResearchConceptsCell polarityPolarity proteinsActin functionCell wall morphogenesisCell polarity proteinsYeast cell polarityPresumptive bud siteCell separation defectATP-sensitive mannerTandem mass spectrometry analysisNonessential proteinsWall morphogenesisMolecular functionsBud sitePolarized localizationSpa2pMass spectrometry analysisSite of growthSaccharomyces cerevisiaeMyo2pCoimmunoprecipitation strategyCell cycleF-actinIndirect interactionsProtein
2000
Role of Actin and Myo2p in Polarized Secretion and Growth ofSaccharomyces cerevisiae
Karpova T, Reck-Peterson S, Elkind N, Mooseker M, Novick P, Cooper J. Role of Actin and Myo2p in Polarized Secretion and Growth ofSaccharomyces cerevisiae. Molecular Biology Of The Cell 2000, 11: 1727-1737. PMID: 10793147, PMCID: PMC14879, DOI: 10.1091/mbc.11.5.1727.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActinsBridged Bicyclo Compounds, HeterocyclicCarrier ProteinsCell DivisionCell PolarityFungal ProteinsGreen Fluorescent ProteinsLuminescent ProteinsMicroscopy, VideoMutationMyosin Heavy ChainsMyosin Type IIMyosin Type VRecombinant ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSchizosaccharomyces pombe ProteinsThiazolesThiazolidinesConceptsActin patch polarizationCell surface growthPolarized secretionFilamentous actinCortical actin patchesClass V myosin Myo2pRole of actinTime-lapse video microscopyIndividual living cellsActin patchesPolarized growthActin cytoskeletonActin cablesMyo2-66Tail domainMyo2pSevere defectsLiving cellsOfSaccharomyces cerevisiaeMotor domainLatrunculinActinOverall growthVideo microscopyQuantitative assay