2007
The Structural And Functional Diversity Of The Myosin Family Of Actin-Based Molecular Motors
Mooseker M, Foth B. The Structural And Functional Diversity Of The Myosin Family Of Actin-Based Molecular Motors. Proteins And Cell Regulation 2007, 7: 1-34. DOI: 10.1007/978-1-4020-6519-4_1.Peer-Reviewed Original Research
2002
MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells
Tyska M, Mooseker M. MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells. Biophysical Journal 2002, 82: 1869-1883. PMID: 11916846, PMCID: PMC1301984, DOI: 10.1016/s0006-3495(02)75537-9.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBiophysical PhenomenaBiophysicsCloning, MolecularCytoskeletonElectrophoresis, Polyacrylamide GelGreen Fluorescent ProteinsHumansKidneyKineticsLLC-PK1 CellsLuminescent ProteinsMicroscopy, ConfocalMicroscopy, FluorescenceMicrovilliMyosin Type IPrecipitin TestsProtein Structure, TertiaryRecombinant Fusion ProteinsSubcellular FractionsSwineTime FactorsTransfectionConceptsTail domainLLC-PK1Actin core bundleKidney epithelial cell lineApical targetingActin dynamicsBrush borderActin turnoverGFP-actinBB populationsEpithelial cell lineActin domainsFluorescence recoveryCl4 cellsRapid turnoverApical surfaceMotor domainCore bundleATP depletionCell linesActinTurnoverCellsDomain
2000
Role of Actin and Myo2p in Polarized Secretion and Growth ofSaccharomyces cerevisiae
Karpova T, Reck-Peterson S, Elkind N, Mooseker M, Novick P, Cooper J. Role of Actin and Myo2p in Polarized Secretion and Growth ofSaccharomyces cerevisiae. Molecular Biology Of The Cell 2000, 11: 1727-1737. PMID: 10793147, PMCID: PMC14879, DOI: 10.1091/mbc.11.5.1727.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActinsBridged Bicyclo Compounds, HeterocyclicCarrier ProteinsCell DivisionCell PolarityFungal ProteinsGreen Fluorescent ProteinsLuminescent ProteinsMicroscopy, VideoMutationMyosin Heavy ChainsMyosin Type IIMyosin Type VRecombinant ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSchizosaccharomyces pombe ProteinsThiazolesThiazolidinesConceptsActin patch polarizationCell surface growthPolarized secretionFilamentous actinCortical actin patchesClass V myosin Myo2pRole of actinTime-lapse video microscopyIndividual living cellsActin patchesPolarized growthActin cytoskeletonActin cablesMyo2-66Tail domainMyo2pSevere defectsLiving cellsOfSaccharomyces cerevisiaeMotor domainLatrunculinActinOverall growthVideo microscopyQuantitative assay
1998
Vesicle-associated brain myosin-V can be activated to catalyze actin-based transport
Evans L, Lee A, Bridgman P, Mooseker M. Vesicle-associated brain myosin-V can be activated to catalyze actin-based transport. Journal Of Cell Science 1998, 111: 2055-2066. PMID: 9645952, DOI: 10.1242/jcs.111.14.2055.Peer-Reviewed Original ResearchConceptsMyosin VVesicle proteinsTotal vesicle proteinSynaptic vesicle proteinsInitial fractionation stepSynaptic vesicle marker proteinActin transportBrain myosin-VOrganelle transportActin filament motilityOrganelle motorFunctional analysisVesicle fractionFunction-blocking antibodiesLocalization studiesMarker proteinsImmunoelectron microscopyMotility assaysMotor domainProteinVesiclesFilament motilityVesicle integrityActinVesicle surfaceUnconventional Myosins in Cell Movement, Membrane Traffic, and Signal Transduction
Mermall V, Post P, Mooseker M. Unconventional Myosins in Cell Movement, Membrane Traffic, and Signal Transduction. Science 1998, 279: 527-533. PMID: 9438839, DOI: 10.1126/science.279.5350.527.Peer-Reviewed Original ResearchConceptsSignal transductionCell movementMembrane trafficMembrane traffickingDisease-causing mutationsCellular functionsMyosin genesImportance of myosinUnconventional myosinMyosin functionCellular levelMolecular motorsMyosin structureTransductionMyosinGenesTraffickingActinMutationsBiochemicalFunctionMembersTargetIdentification
1996
Mapping of Unconventional Myosins in Mouse and Human
Hasson T, Skowron J, Gilbert D, Avraham K, Perry W, Bement W, Anderson B, Sherr E, Chen Z, Greene L, Ward D, Corey D, Mooseker M, Copeland N, Jenkins N. Mapping of Unconventional Myosins in Mouse and Human. Genomics 1996, 36: 431-439. PMID: 8884266, DOI: 10.1006/geno.1996.0488.Peer-Reviewed Original ResearchThe actin-related proteins
Frankel S, Mooseker M. The actin-related proteins. Current Opinion In Cell Biology 1996, 8: 30-37. PMID: 8791406, DOI: 10.1016/s0955-0674(96)80045-7.Peer-Reviewed Original ResearchConceptsActin-related proteinsHsp/Hsc70Family of proteinsClear sequence homologyPrimary structural dataCell cycle proteinsStructural dataFunctional diversitySugar kinasesSequence homologyActin filamentsCycle proteinsFunctional contextProteinActinBiochemical analysisChromatinHsc70FamilyHomologyKinaseStructural elementsMicrotubulesMembersDiversity
1995
Molecular motors, membrane movements and physiology: emerging roles for myosins
Hasson T, Mooseker M. Molecular motors, membrane movements and physiology: emerging roles for myosins. Current Opinion In Cell Biology 1995, 7: 587-594. PMID: 7495580, DOI: 10.1016/0955-0674(95)80017-4.Peer-Reviewed Original ResearchConceptsMembrane protein functionMolecular motorsProtein functionSignal transductionNovel myosinOrganelle transportATP hydrolysisActin filamentsCell locomotionLarge familyMembrane movementUbiquitous associationMechanical forcesActinMyosinMembrane phenomenaTransductionMechanoenzymesMechanoregulationPhysiologyRolePhagocytosisMembraneFilamentsFamily
1994
Identification of a Divergent Actin-related Protein in Drosophila
Frankel S, Heintzelman M, Artavanis-Tsakonas S, Mooseker M. Identification of a Divergent Actin-related Protein in Drosophila. Journal Of Molecular Biology 1994, 235: 1351-1356. PMID: 8308899, DOI: 10.1006/jmbi.1994.1090.Peer-Reviewed Original ResearchConceptsActin-related proteinsAmino acid identityPrimary sequence homologyDivergent actinConventional actinPolytene chromosomesEarly embryogenesisAcid identityX chromosomeSequence homologyGenomic DNAOverall divergenceSequence insertionNew isotypeActinDrosophilaProteinEmbryogenesisChromosomesHomologyGenesStages of developmentTranscriptsUnique locationLater stagesDifferential regulation of skeletal muscle myosin‐II and brush border myosin‐I enzymology and mechanochemistry by bacterially produced tropomyosin isoforms
Fanning A, Wolenski J, Mooseker M, Izant J. Differential regulation of skeletal muscle myosin‐II and brush border myosin‐I enzymology and mechanochemistry by bacterially produced tropomyosin isoforms. Cytoskeleton 1994, 29: 29-45. PMID: 7820856, DOI: 10.1002/cm.970290104.Peer-Reviewed Original ResearchMeSH KeywordsActinsAmino Acid SequenceAnimalsCa(2+) Mg(2+)-ATPaseCarrier ProteinsCell MovementChick EmbryoDNA, ComplementaryEscherichia coliIntestinal MucosaMicrofilament ProteinsMicrovilliMolecular Sequence DataMolecular WeightMuscle ProteinsMyosinsProtein BindingRecombinant Fusion ProteinsRecombinant ProteinsSequence HomologySpecies SpecificityTropomyosinVertebratesXenopusConceptsAlpha-tropomyosinMuscle myosin IIMyosin enzymologyMyosin isoformsTwo- toStriated MuscleMgATPase activityDifferential regulationBrush border myosinDetectable effectIsoformsLow affinityChimeric tropomyosinsMuscle tropomyosinMuscle alpha-tropomyosinSkeletal muscle myosin IIActinTropomyosin isoformsMuscle myosinF-actin
1990
Binding of brush border myosin I to phospholipid vesicles.
Hayden S, Wolenski J, Mooseker M. Binding of brush border myosin I to phospholipid vesicles. Journal Of Cell Biology 1990, 111: 443-451. PMID: 2143194, PMCID: PMC2116197, DOI: 10.1083/jcb.111.2.443.Peer-Reviewed Original ResearchConceptsBB myosin IMyosin IBrush border myosin IMyosin I heavy chainMembrane-binding domainActin filament corePhospholipid vesiclesATP-sensitive mannerPlasma membraneIntestinal epithelial cellsF-actinMembrane interactionsPhosphatidylglycerol vesiclesFree proteinCOOH-terminalAnionic phospholipidsImmunoblot analysisVesiclesEpithelial cellsImmunoblot stainingM. SimilarHeavy chainActinStructural informationNeutral phospholipids
1986
Calcium and the Regulation of Cytoskeletal Assembly, Structure and Contractility
Mooseker M, Coleman T, Conzelman K. Calcium and the Regulation of Cytoskeletal Assembly, Structure and Contractility. Novartis Foundation Symposia 1986, 122: 232-249. PMID: 3792141, DOI: 10.1002/9780470513347.ch14.Peer-Reviewed Original ResearchConceptsActin-binding proteinsCytoskeletal assemblyActin filament interactionsCase of actinSubset of proteinsInteraction of actinActin assemblyCytoskeletal networkCytoskeletal structuresMode of Ca2Dependent regulationActin filamentsSpecific functionsProteinRegulationCentral roleActinFilament interactionAssemblyMyosin interactionCa2Functional classInteractionMembrane
1982
The Mechanism of Actin-Filament Assembly and Cross-Linking
Pollard T, Aebi U, Cooper J, Elzinga M, Fowler W, Griffith L, Herman I, Heuser J, Isenberg G, Kiehart D, Levy J, MacLean-Fletcher S, Maupin P, Mooseker M, Runge M, Smith P, Tseng P. The Mechanism of Actin-Filament Assembly and Cross-Linking. 1982, 15-44. DOI: 10.1007/978-1-4684-4037-9_2.Peer-Reviewed Original ResearchActin filamentsActin filament assemblyLocal contractile forcesRole of actinCertain enzyme systemsEukaryotic cellsFilaments of actinCellular motilityStructural proteinsCyto-skeletonMajor proteinsCell surfaceMost cellsMajor structural elementsProteinEnzyme systemActinContractile proteinsCytoplasmContractile apparatusFilamentsCellsInternal scaffoldingOrganellesStructural elements
1973
THE POLYMERIZATION OF ACTIN: ITS ROLE IN THE GENERATION OF THE ACROSOMAL PROCESS OF CERTAIN ECHINODERM SPERM
Tilney L, Hatano S, Ishikawa H, Mooseker M. THE POLYMERIZATION OF ACTIN: ITS ROLE IN THE GENERATION OF THE ACROSOMAL PROCESS OF CERTAIN ECHINODERM SPERM. Journal Of Cell Biology 1973, 59: 109-126. PMID: 4356568, PMCID: PMC2110911, DOI: 10.1083/jcb.59.1.109.Peer-Reviewed Original ResearchConceptsSperm actinAcrosomal processGel electrophoresisUnreacted spermSodium dodecyl sulfate gel electrophoresisDodecyl sulfate gel electrophoresisMuscle myosinMuscle F-actinPolymerization of actinHeavy meromyosin bindingSulfate gel electrophoresisEchinoderm spermF-actinSDS gel electrophoresisMonomeric stateDetergent Triton XSkeletal muscle myosinActinAcrosomal regionBundles of microfilamentsAcrosomal capMicrofilamentsSame molecular weightSperm extractsEgg jelly