Knockout of the PKC Substrate Pleckstrin Causes Pleomorphic Defects in Platelets, Lymphocytes and Granulocytes.
Lian L, Wang Y, Chen X, Bach T, Lenox L, Zhu P, Flick M, Scott E, Degen J, Freedman B, Koretzky G, Lemmon M, Abrams C. Knockout of the PKC Substrate Pleckstrin Causes Pleomorphic Defects in Platelets, Lymphocytes and Granulocytes. Blood 2006, 108: 394. DOI: 10.1182/blood.v108.11.394.394.Peer-Reviewed Original ResearchSecond messengerPI3KPI3K inhibitorsCarboxyl-terminal pleckstrin homology domainPleckstrin homology domainPhospholipid second messengerWild-type cellsTotal cellular proteinMultiple hematopoietic lineagesK inhibitorsMendelian inheritance patternWild-type plateletsPhosphorylated pleckstrinHomology domainCytoskeletal dynamicsRole of PKCDEP domainExocytosis defectsCellular proteinsCytoskeletal defectsCytoskeletal reorganizationMembrane extensionsReactive oxygen speciesHematopoietic lineagesPleckstrin