2004
The p21-activated Protein Kinase-related Kinase Cla4 Is a Coincidence Detector of Signaling by Cdc42 and Phosphatidylinositol 4-Phosphate*
Wild AC, Yu JW, Lemmon MA, Blumer KJ. The p21-activated Protein Kinase-related Kinase Cla4 Is a Coincidence Detector of Signaling by Cdc42 and Phosphatidylinositol 4-Phosphate*. Journal Of Biological Chemistry 2004, 279: 17101-17110. PMID: 14766750, DOI: 10.1074/jbc.m314035200.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAmino Acid SequenceCdc42 GTP-Binding ProteinCell MembraneDose-Response Relationship, DrugEscherichia coliGenotypeGreen Fluorescent ProteinsImmunoblottingKineticsLipid MetabolismLuminescent ProteinsMitosisModels, GeneticMolecular Sequence DataMutationP21-Activated KinasesPhosphatidylinositol PhosphatesPlasmidsPoint MutationProtein BindingProtein Serine-Threonine KinasesProtein Structure, TertiaryRecombinant Fusion ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidSignal TransductionSurface Plasmon ResonanceTemperatureConceptsPleckstrin homologyPH domainRho-type GTPase Cdc42P21-activated protein kinaseMitotic exit networkPlasma membrane poolSignal transduction pathwaysPhosphoinositide speciesGolgi poolCell morphogenesisEukaryotic cellsGTPase Cdc42Cdc42 bindingKinase mutantsMammalian cellsCla4Protein kinaseTransduction pathwaysCoincidence detectorMembrane poolPlasma membraneCdc42Kinase activityPI4PBiological processes
2003
Molecular Determinants of tGolgin-1 Function
Yoshino A, Marks M, Lemmon M. Molecular Determinants of tGolgin-1 Function. 2003 DOI: 10.21236/ada418143.Peer-Reviewed Original ResearchTrans-Golgi networkGRIP domainMammalian cellsC-terminal GRIP domainDynein/dynactin complexGRIP domain proteinsPeripheral membrane proteinsGTPase cascadeDomain proteinsDynactin complexGolgi networkMembrane proteinsCell motilityTurn bindsGolgi complexProtein resultsMolecular determinantsProteinTumor developmentOrthologuesCellsDomainRNAiEndosomesYeast
2002
Molecular Determinants of tGolgin-1 Function
Yoshino A, Marks M, Lemmon M. Molecular Determinants of tGolgin-1 Function. 2002 DOI: 10.21236/ada408101.Peer-Reviewed Original ResearchTrans-Golgi networkGRIP domainC-terminal GRIP domainGRIP domain proteinsPeripheral membrane proteinsN-terminal domainDomain proteinsGolgi networkTGN structureMammalian cellsGolgin-97Membrane proteinsCell motilityMolecular basisGolgi complexMolecular determinantsBroader roleCoil regionProteinTumor metastasisProtein contentCellsDomainRNAiMicrotubules
1998
Specificity and Promiscuity in Phosphoinositide Binding by Pleckstrin Homology Domains*
Kavran J, Klein D, Lee A, Falasca M, Isakoff S, Skolnik E, Lemmon M. Specificity and Promiscuity in Phosphoinositide Binding by Pleckstrin Homology Domains*. Journal Of Biological Chemistry 1998, 273: 30497-30508. PMID: 9804818, DOI: 10.1074/jbc.273.46.30497.Peer-Reviewed Original ResearchConceptsPleckstrin homology domainPH domainGrp1 PH domainD-myo-inositolParticular phosphoinositidesPhosphoinositide bindingHomology domainDependent membrane recruitmentDifferent PH domainsPH domain bindsSmall protein modulesSoluble inositol phosphatesMembrane recruitmentDomain bindsProtein modulesSpecific phosphoinositideMammalian cellsPlasma membraneSingle speciesAbundant speciesMultiple phosphoinositidesCellular membranesPhosphoinositidePI 3Clear specificity