2004
Maitotoxin Induces Biphasic Interleukin-1β Secretion and Membrane Blebbing in Murine Macrophages
Verhoef P, Kertesy S, Estacion M, Schilling W, Dubyak G. Maitotoxin Induces Biphasic Interleukin-1β Secretion and Membrane Blebbing in Murine Macrophages. Molecular Pharmacology 2004, 66: 909-920. PMID: 15385641, DOI: 10.1124/mol.66.4.909.Peer-Reviewed Original ResearchConceptsMTX treatmentMIL-1betaIL-1betaCytolytic releaseProIL-1betaProinflammatory cytokine interleukin-1betaProinflammatory cytokine secretionCytokine interleukin-1betaMature IL-1betaMurine macrophagesInterleukin-1β secretionCytokine releaseInflammatory leukocytesCytokine secretionP2X7 receptorInterleukin-1betaInflammatory macrophagesExtracellular Ca2Extracellular glycineIntracellular Ca2Vivo responseMacrophagesCation channelsSecretionLytic phaseActivation of Human TRPC6 Channels by Receptor Stimulation*
Estacion M, Li S, Sinkins W, Gosling M, Bahra P, Poll C, Westwick J, Schilling W. Activation of Human TRPC6 Channels by Receptor Stimulation*. Journal Of Biological Chemistry 2004, 279: 22047-22056. PMID: 15023993, DOI: 10.1074/jbc.m402320200.Peer-Reviewed Original ResearchMeSH KeywordsCalcium ChannelsCarbacholCell LineCell MembraneCholinergic AgonistsDNA, ComplementaryDose-Response Relationship, DrugElectrophysiologyEnzyme InhibitorsHumansIonomycinIonophoresKineticsLac OperonMembrane PotentialsProtein BindingTetradecanoylphorbol AcetateThapsigarginTime FactorsTransfectionTRPC Cation ChannelsTRPC6 Cation ChannelConceptsReceptor stimulationTRPC6 currentsTRPC6 channelsSteep dose-response relationshipEffect of carbacholConcentrations of carbacholSteep concentration-response relationshipReceptor-mediated eventsActivation of TRPC6Channel activityDose-response relationshipWhole-cell membrane currentsSignificant acute effectConcentration-response relationshipCell membrane currentsAcute effectsCarbacholGeneration of DAGHuman embryonic kidney cellsPrior stimulationPatch pipetteTRPC6 activityEmbryonic kidney cellsMembrane currentsMembrane depolarization
1993
Ca2+ influx via T-type channels modulates PDGF-induced replication of mouse fibroblasts
Wang Z, Estacion M, Mordan L. Ca2+ influx via T-type channels modulates PDGF-induced replication of mouse fibroblasts. American Journal Of Physiology 1993, 265: c1239-c1246. PMID: 8238477, DOI: 10.1152/ajpcell.1993.265.5.c1239.Peer-Reviewed Original ResearchConceptsVoltage-gated calcium channelsExtracellular calcium influxCalcium influxNordihydroguariaretic acidCalcium currentWhole-cell patch-clamp techniqueCell patch-clamp techniqueIntracellular calcium increaseInward calcium currentCell proliferationIntracellular calcium concentrationT-type channelsAddition of prostaglandinPlatelet-derived growth factorMean inhibitory dosePatch-clamp techniqueC3H/10T1/2 mouse fibroblastsInhibitory doseCalcium channelsCalcium increaseSustained increaseGrowth factorInhibitory effectCalcium concentrationCell cycle progression
1991
Characterization of ion channels seen in subconfluent human dermal fibroblasts.
Estacion M. Characterization of ion channels seen in subconfluent human dermal fibroblasts. The Journal Of Physiology 1991, 436: 579-601. PMID: 1712040, PMCID: PMC1181523, DOI: 10.1113/jphysiol.1991.sp018568.Peer-Reviewed Original ResearchConceptsIon channelsHuman dermal fibroblastsBradykinin-stimulated cellsDermal fibroblastsDifferent ion channelsSurprising diversityIon channel modulationChannel phenotypeInward rectifierGap junctionsUnstimulated cellsUnexcitable cellsChannel modulationActivation kineticsGuanosine 5'FibroblastsCellsPhenotypePipette solutionCl- currentFast inactivationExpressionAdditional subtypesPatch-clamp techniqueLow number