2010
Can robots patch‐clamp as well as humans? Characterization of a novel sodium channel mutation
Estacion M, Choi JS, Eastman EM, Lin Z, Li Y, Tyrrell L, Yang Y, Dib‐Hajj S, Waxman SG. Can robots patch‐clamp as well as humans? Characterization of a novel sodium channel mutation. The Journal Of Physiology 2010, 588: 1915-1927. PMID: 20123784, PMCID: PMC2901980, DOI: 10.1113/jphysiol.2009.186114.Peer-Reviewed Original Research
1999
Stimulation of Drosophila TrpL by capacitative Ca2+ entry.
Estacion M, Sinkins W, Schilling W. Stimulation of Drosophila TrpL by capacitative Ca2+ entry. Biochemical Journal 1999, 341 ( Pt 1): 41-9. PMID: 10377243, PMCID: PMC1220328, DOI: 10.1042/0264-6021:3410041.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBariumBiological TransportCalciumCalcium-Transporting ATPasesCalmodulinCalmodulin-Binding ProteinsCations, DivalentDrosophilaDrosophila ProteinsEndoplasmic ReticulumHumansIon Channel GatingIon ChannelsLanthanumMembrane ProteinsPatch-Clamp TechniquesRecombinant Fusion ProteinsSpodopteraStrontiumThapsigarginTransient Receptor Potential ChannelsConceptsCapacitative Ca2Internal Ca2Receptor-activated channelsCell-attached patch recordingsTRP-like proteinsNon-selective cation channelsCytosolic free Ca2CBS-2Fura-2Cation entryPatch recordingsHuman TRPC1Single-channel activityDrosophila TRPLTRPL activityFree Ca2Dependent mechanismCation influxGel overlay experimentsCation channelsPhotoreceptor cellsChannel activityPhospholipase CTRPC1TRPL channel activity
1993
Competence induction by PDGF requires sustained calcium influx by a mechanism distinct from storage-dependent calcium influx
Estacion M, Mordan L. Competence induction by PDGF requires sustained calcium influx by a mechanism distinct from storage-dependent calcium influx. Cell Calcium 1993, 14: 439-454. PMID: 8395338, DOI: 10.1016/0143-4160(93)90003-o.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBucladesineCalciumCalcium ChannelsCell CompartmentationCell DivisionCell LineCell MembraneColforsinContact InhibitionDNA ReplicationDrug SynergismExtracellular SpaceFibroblastsGallic AcidInsulinIon Channel GatingLanthanumMiceMice, Inbred C3HNifedipinePlatelet-Derived Growth FactorS PhaseSignal TransductionTetradecanoylphorbol AcetateConceptsCalcium influxIntracellular storage sitesExtracellular calciumCalcium influx pathwaySustained calcium influxIntracellular calcium concentrationExtracellular calcium influxRelease of calciumPercentage of cellsC3H 10T1/2 mouse fibroblastsPDGF exposureSustained elevationTMB-8Influx pathwayBiphasic increaseSustained increaseDibutyryl cGMPCalcium concentrationS phasePDGFDibutyryl cAMPCell replicationSelective inhibitionReplicative competenceProgressionExpression of voltage-gated calcium channels correlates with PDGF-stimulated calcium influx and depends upon cell density in C3H 10T12 mouse fibroblasts
Estacion M, Mordan L. Expression of voltage-gated calcium channels correlates with PDGF-stimulated calcium influx and depends upon cell density in C3H 10T12 mouse fibroblasts. Cell Calcium 1993, 14: 161-171. PMID: 8384532, DOI: 10.1016/0143-4160(93)90085-k.Peer-Reviewed Original ResearchConceptsVoltage-gated calcium channelsCalcium channelsT-type voltage-gated calcium channelsWhole-cell patch-clamp recordingsWhole-cell patch-clamp techniqueCell patch-clamp techniquePatch-clamp recordingsPatch-clamp techniqueMembrane calcium channelsIntracellular calcium signalsPlasma membrane calcium channelsC3H 10T1/2 mouse fibroblastsCalcium currentCalcium influxFura-2Extracellular calciumCalcium poolSustained influxGrowth factorInflux componentFraction of cellsCalcium signalsCell densityMouse fibroblastsConfluent monolayers
1991
Characterization of ion channels seen in subconfluent human dermal fibroblasts.
Estacion M. Characterization of ion channels seen in subconfluent human dermal fibroblasts. The Journal Of Physiology 1991, 436: 579-601. PMID: 1712040, PMCID: PMC1181523, DOI: 10.1113/jphysiol.1991.sp018568.Peer-Reviewed Original ResearchConceptsIon channelsHuman dermal fibroblastsBradykinin-stimulated cellsDermal fibroblastsDifferent ion channelsSurprising diversityIon channel modulationChannel phenotypeInward rectifierGap junctionsUnstimulated cellsUnexcitable cellsChannel modulationActivation kineticsGuanosine 5'FibroblastsCellsPhenotypePipette solutionCl- currentFast inactivationExpressionAdditional subtypesPatch-clamp techniqueLow number