2005
Identification and localization of TRPC channels in the rat kidney
Goel M, Sinkins W, Zuo C, Estacion M, Schilling W. Identification and localization of TRPC channels in the rat kidney. American Journal Of Physiology. Renal Physiology 2005, 290: f1241-f1252. PMID: 16303855, DOI: 10.1152/ajprenal.00376.2005.Peer-Reviewed Original ResearchConceptsTubular cellsDuct cellsTransient receptor potential channelsG protein-coupled membrane receptorsPresence of TRPC1Aquaporin-2Western blot analysisMesangial cell culturesKidney sectionsProximal tubulesOuter medullaRat kidneyTRPC channelsRenal nephronKidney lysateTRPC3Immunopositive labelingTRPC1PLC-dependent signalingRat kidney lysateInner medullaAffinity-purified antibodiesPrinciple cellsPolarized culturesCell lines
2003
P2X7 Receptor-Dependent Blebbing and the Activation of Rho-Effector Kinases, Caspases, and IL-1β Release
Verhoef P, Estacion M, Schilling W, Dubyak G. P2X7 Receptor-Dependent Blebbing and the Activation of Rho-Effector Kinases, Caspases, and IL-1β Release. The Journal Of Immunology 2003, 170: 5728-5738. PMID: 12759456, DOI: 10.4049/jimmunol.170.11.5728.Peer-Reviewed Original ResearchMeSH Keywords1-(5-Isoquinolinesulfonyl)-2-MethylpiperazineAdenosine TriphosphateAmidesAnimalsCaspase InhibitorsCaspasesCell LineEnzyme ActivationExtracellular SpaceHumansInterleukin-1Intracellular Signaling Peptides and ProteinsMacrophagesMiceProtein Serine-Threonine KinasesPseudopodiaPyridinesReceptors, Purinergic P2Receptors, Purinergic P2X7RhoA GTP-Binding ProteinRho-Associated KinasesSignal TransductionConceptsPlasma membrane organizationRho effector kinasesHuman embryonic kidney 293 cellsMurine macrophagesEmbryonic kidney 293 cellsPlasma membrane morphologyInhibitor KN-62Activation of RhoAKidney 293 cellsMembrane organizationTime-lapse moviesIL-1 beta releaseBlebbingKN-62Cation channel activationPore formationBiochemical analysisActivation signalsATPATP additionATP treatmentChannel activationAgonist BzATPATP concentrationCells
2001
Regulation of Drosophila TRPL Channels by Immunophilin FKBP59*
Goel M, Garcia R, Estacion M, Schilling W. Regulation of Drosophila TRPL Channels by Immunophilin FKBP59*. Journal Of Biological Chemistry 2001, 276: 38762-38773. PMID: 11514552, DOI: 10.1074/jbc.m104125200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsAmino Acid SequenceAnimalsBinding SitesCalciumCalmodulin-Binding ProteinsCationsCell LineChelating AgentsDNA, ComplementaryDrosophilaDrosophila ProteinsEgtazic AcidElectrophysiologyGene LibraryGlutathione TransferaseHumansImmunohistochemistryInsectaMembrane ProteinsMicroscopy, FluorescenceMolecular Sequence DataMutationPhotoreceptor Cells, InvertebratePolymerase Chain ReactionPrecipitin TestsProtein BindingProtein Structure, TertiaryRecombinant ProteinsSequence Homology, Amino AcidSignal TransductionTacrolimus Binding ProteinsTime FactorsTransient Receptor Potential ChannelsTwo-Hybrid System TechniquesConceptsS2 cellsTRPL channelsDrosophila head cDNA libraryDrosophila photoreceptor cellsHuman FK506-binding proteinMultimeric signaling complexesTwo-hybrid screenDrosophila S2 cellsDrosophila TRPL channelsSf9 cell lysatesFK506-binding proteinChannel activitySite-directed mutagenesisCytoplasmic membrane surfaceFura-2 assayTransient receptor potentialDrosophila homologPermeable cation channelSignaling ComplexNovel proteinCDNA librarySf9 cellsINADCytoplasmic mouthChannel regulation
1997
PDGF-Stimulated Calcium Influx Changes During In Vitro Cell Transformation
Estacion M, Mordan L. PDGF-Stimulated Calcium Influx Changes During In Vitro Cell Transformation. Cellular Signalling 1997, 9: 363-366. PMID: 9376215, DOI: 10.1016/s0898-6568(96)00184-2.Peer-Reviewed Original ResearchConceptsT-type calcium channelsCalcium signalsIntracellular calcium signalsC3H 10T1/2 mouse fibroblastsCalcium channelsPreneoplastic clonesMarked reductionGrowth factorMetabolism changesVitro Cell TransformationCell transformationColonies of cellsElectrophysiology measurementsInflux changeCellsMouse fibroblastsMutations Causing Achondroplasia and Thanatophoric Dysplasia Alter bFGF-Induced Calcium Signals in Human Diploid Fibroblasts
Nguyen H, Estacion M, Gargus J. Mutations Causing Achondroplasia and Thanatophoric Dysplasia Alter bFGF-Induced Calcium Signals in Human Diploid Fibroblasts. Human Molecular Genetics 1997, 6: 681-688. PMID: 9158142, DOI: 10.1093/hmg/6.5.681.Peer-Reviewed Original ResearchMeSH KeywordsAchondroplasiaBradykininCalciumCell LineDiploidyFibroblast Growth Factor 2FibroblastsHomozygoteHumansMutationProtein-Tyrosine KinasesReceptor Protein-Tyrosine KinasesReceptor, Fibroblast Growth Factor, Type 1Receptor, Fibroblast Growth Factor, Type 2Receptor, Fibroblast Growth Factor, Type 3Receptors, Fibroblast Growth FactorRNA SplicingSignal TransductionThanatophoric DysplasiaConceptsThanatophoric dysplasiaCalcium signalsBasic fibroblast growth factorCell linesCalcium-imaging analysesFibroblast growth factorFibroblast growth factor receptor (FGFR) gene familyHuman diploid fibroblastsPathophysiological mechanismsIntracellular calciumBlinded studyFGFR3 mutationsUnresponsive linesReceptor functionHereditary disorderTransient increaseGrowth factorDiploid fibroblastsControl cellsBone developmentFibroblast cell lineCalcium wavesReceptor gene familyHeterogeneous signalsAchondroplasia
1993
Competence induction by PDGF requires sustained calcium influx by a mechanism distinct from storage-dependent calcium influx
Estacion M, Mordan L. Competence induction by PDGF requires sustained calcium influx by a mechanism distinct from storage-dependent calcium influx. Cell Calcium 1993, 14: 439-454. PMID: 8395338, DOI: 10.1016/0143-4160(93)90003-o.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBucladesineCalciumCalcium ChannelsCell CompartmentationCell DivisionCell LineCell MembraneColforsinContact InhibitionDNA ReplicationDrug SynergismExtracellular SpaceFibroblastsGallic AcidInsulinIon Channel GatingLanthanumMiceMice, Inbred C3HNifedipinePlatelet-Derived Growth FactorS PhaseSignal TransductionTetradecanoylphorbol AcetateConceptsCalcium influxIntracellular storage sitesExtracellular calciumCalcium influx pathwaySustained calcium influxIntracellular calcium concentrationExtracellular calcium influxRelease of calciumPercentage of cellsC3H 10T1/2 mouse fibroblastsPDGF exposureSustained elevationTMB-8Influx pathwayBiphasic increaseSustained increaseDibutyryl cGMPCalcium concentrationS phasePDGFDibutyryl cAMPCell replicationSelective inhibitionReplicative competenceProgression
1990
Inhibition of voltage-dependent Na+ current in cell-fusion hybrids containing activated c-Ha-ras
Estacion M. Inhibition of voltage-dependent Na+ current in cell-fusion hybrids containing activated c-Ha-ras. The Journal Of Membrane Biology 1990, 113: 169-175. PMID: 2157017, DOI: 10.1007/bf01872890.Peer-Reviewed Original Research