Characterization of ion channels seen in subconfluent human dermal fibroblasts.
Estacion M. Characterization of ion channels seen in subconfluent human dermal fibroblasts. The Journal Of Physiology 1991, 436: 579-601. PMID: 1712040, PMCID: PMC1181523, DOI: 10.1113/jphysiol.1991.sp018568.Peer-Reviewed Original ResearchConceptsIon channelsHuman dermal fibroblastsBradykinin-stimulated cellsDermal fibroblastsDifferent ion channelsSurprising diversityIon channel modulationChannel phenotypeInward rectifierGap junctionsUnstimulated cellsUnexcitable cellsChannel modulationActivation kineticsGuanosine 5'FibroblastsCellsPhenotypePipette solutionCl- currentFast inactivationExpressionAdditional subtypesPatch-clamp techniqueLow numberAcute electrophysiological responses of bradykinin‐stimulated human fibroblasts.
Estacion M. Acute electrophysiological responses of bradykinin‐stimulated human fibroblasts. The Journal Of Physiology 1991, 436: 603-620. PMID: 1648132, PMCID: PMC1181524, DOI: 10.1113/jphysiol.1991.sp018569.Peer-Reviewed Original ResearchConceptsFura-2 fluorescencePipette solutionIon substitution experimentsGTP gamma SReversal potentialBradykinin stimulationPresence of GTPHuman dermal fibroblastsGamma SPatch-clamp techniqueNon-specific cation channelsBradykinin additionDermal fibroblastsIntracellular calciumLittle voltage dependencePatch-clamped cellsTail current analysisAcute responseInward currentsCalcium ionophoreOutward currentsNear baselineCation channelsElectrophysiological responsesBradykinin