2004
Association of Immunophilins with Mammalian TRPC Channels*
Sinkins W, Goel M, Estacion M, Schilling W. Association of Immunophilins with Mammalian TRPC Channels*. Journal Of Biological Chemistry 2004, 279: 34521-34529. PMID: 15199065, DOI: 10.1074/jbc.m401156200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBaculoviridaeBrainCalcium ChannelsCell LineDose-Response Relationship, DrugElectrophysiologyGlutamineHumansImmunoblottingImmunophilinsInsectaMutationPeptidesPrecipitin TestsProlineProtein BindingProtein Structure, TertiaryRatsTacrolimus Binding Protein 1ATacrolimus Binding ProteinsTime FactorsTRPC Cation ChannelsConceptsTRPC channel proteinsChannel proteinsMammalian TRPC channelsTRPC channelsHEK cellsBinding of FKBP12Rat brain lysatesImmunosuppressant drug FK506Receptor-mediated activationSf9 cellsAccessory proteinsRat cerebral cortexImmunophilinsDrug FK506Brain lysatesFKBP52FKBP12Membrane lysatesGln mutationINADProteinFKBP59SignalplexTRPC1Cerebral cortexActivation of Human TRPC6 Channels by Receptor Stimulation*
Estacion M, Li S, Sinkins W, Gosling M, Bahra P, Poll C, Westwick J, Schilling W. Activation of Human TRPC6 Channels by Receptor Stimulation*. Journal Of Biological Chemistry 2004, 279: 22047-22056. PMID: 15023993, DOI: 10.1074/jbc.m402320200.Peer-Reviewed Original ResearchMeSH KeywordsCalcium ChannelsCarbacholCell LineCell MembraneCholinergic AgonistsDNA, ComplementaryDose-Response Relationship, DrugElectrophysiologyEnzyme InhibitorsHumansIonomycinIonophoresKineticsLac OperonMembrane PotentialsProtein BindingTetradecanoylphorbol AcetateThapsigarginTime FactorsTransfectionTRPC Cation ChannelsTRPC6 Cation ChannelConceptsReceptor stimulationTRPC6 currentsTRPC6 channelsSteep dose-response relationshipEffect of carbacholConcentrations of carbacholSteep concentration-response relationshipReceptor-mediated eventsActivation of TRPC6Channel activityDose-response relationshipWhole-cell membrane currentsSignificant acute effectConcentration-response relationshipCell membrane currentsAcute effectsCarbacholGeneration of DAGHuman embryonic kidney cellsPrior stimulationPatch pipetteTRPC6 activityEmbryonic kidney cellsMembrane currentsMembrane depolarization
2001
Regulation of Drosophila TRPL Channels by Immunophilin FKBP59*
Goel M, Garcia R, Estacion M, Schilling W. Regulation of Drosophila TRPL Channels by Immunophilin FKBP59*. Journal Of Biological Chemistry 2001, 276: 38762-38773. PMID: 11514552, DOI: 10.1074/jbc.m104125200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsAmino Acid SequenceAnimalsBinding SitesCalciumCalmodulin-Binding ProteinsCationsCell LineChelating AgentsDNA, ComplementaryDrosophilaDrosophila ProteinsEgtazic AcidElectrophysiologyGene LibraryGlutathione TransferaseHumansImmunohistochemistryInsectaMembrane ProteinsMicroscopy, FluorescenceMolecular Sequence DataMutationPhotoreceptor Cells, InvertebratePolymerase Chain ReactionPrecipitin TestsProtein BindingProtein Structure, TertiaryRecombinant ProteinsSequence Homology, Amino AcidSignal TransductionTacrolimus Binding ProteinsTime FactorsTransient Receptor Potential ChannelsTwo-Hybrid System TechniquesConceptsS2 cellsTRPL channelsDrosophila head cDNA libraryDrosophila photoreceptor cellsHuman FK506-binding proteinMultimeric signaling complexesTwo-hybrid screenDrosophila S2 cellsDrosophila TRPL channelsSf9 cell lysatesFK506-binding proteinChannel activitySite-directed mutagenesisCytoplasmic membrane surfaceFura-2 assayTransient receptor potentialDrosophila homologPermeable cation channelSignaling ComplexNovel proteinCDNA librarySf9 cellsINADCytoplasmic mouthChannel regulation