2006
Palytoxin-induced cell death cascade in bovine aortic endothelial cells
Schilling W, Snyder D, Sinkins W, Estacion M. Palytoxin-induced cell death cascade in bovine aortic endothelial cells. American Journal Of Physiology - Cell Physiology 2006, 291: c657-c667. PMID: 16672692, DOI: 10.1152/ajpcell.00063.2006.Peer-Reviewed Original ResearchMeSH KeywordsAcrylamidesAnimalsAortaCalciumCattleCell DeathCells, CulturedCnidarian VenomsComputer SystemsDose-Response Relationship, DrugDrug Administration ScheduleEndothelial CellsEthidiumFluorescent DyesGreen Fluorescent ProteinsIntracellular MembranesMicroscopy, VideoOsmolar ConcentrationOuabainPertussis ToxinPropidiumConceptsAortic endothelial cellsBovine aortic endothelial cellsEndothelial cellsConcentration-dependent increaseCell deathAddition of ouabainMonovalent cation channelCell death cascadeCell lysisMarine toxin palytoxinCation channelsOuabainOncotic cell deathEB uptakeATPase pumpDeath cascadeTime-lapse video microscopyPropidium iodideDeathYO-PRO-1ExtracellularEthidium bromideDownstream eventsRapid uptake
2004
Maitotoxin Induces Biphasic Interleukin-1β Secretion and Membrane Blebbing in Murine Macrophages
Verhoef P, Kertesy S, Estacion M, Schilling W, Dubyak G. Maitotoxin Induces Biphasic Interleukin-1β Secretion and Membrane Blebbing in Murine Macrophages. Molecular Pharmacology 2004, 66: 909-920. PMID: 15385641, DOI: 10.1124/mol.66.4.909.Peer-Reviewed Original ResearchConceptsMTX treatmentMIL-1betaIL-1betaCytolytic releaseProIL-1betaProinflammatory cytokine interleukin-1betaProinflammatory cytokine secretionCytokine interleukin-1betaMature IL-1betaMurine macrophagesInterleukin-1β secretionCytokine releaseInflammatory leukocytesCytokine secretionP2X7 receptorInterleukin-1betaInflammatory macrophagesExtracellular Ca2Extracellular glycineIntracellular Ca2Vivo responseMacrophagesCation channelsSecretionLytic phase