2009
Maitotoxin converts the plasmalemmal Ca2+ pump into a Ca2+-permeable nonselective cation channel
Sinkins W, Estacion M, Prasad V, Goel M, Shull G, Kunze D, Schilling W. Maitotoxin converts the plasmalemmal Ca2+ pump into a Ca2+-permeable nonselective cation channel. American Journal Of Physiology - Cell Physiology 2009, 297: c1533-c1543. PMID: 19794142, PMCID: PMC2793065, DOI: 10.1152/ajpcell.00252.2009.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsAnimals, Genetically ModifiedCalciumCation Transport ProteinsCationsCell MembraneCells, CulturedDown-RegulationElectric ConductivityFibroblastsHumansIon ChannelsKidneyMarine ToxinsMiceOxocinsPermeabilityPlasma Membrane Calcium-Transporting ATPasesRNA, Small InterferingSpodopteraUp-RegulationConceptsPermeable nonselective cation channelNonselective cation channelsCation channelsSpodoptera frugiperda (Sf9) insect cellsHEK cellsMouse embryonic fibroblastsHuman embryonic kidney 293 cellsEmbryonic kidney 293 cellsKidney 293 cellsInsect cellsEmbryonic fibroblastsWhole-cell membrane currentsMolecular identityCell membrane currentsCell typesPMCACytosolic freeMarine toxinsEnhanced expressionWhole-cell currentsPlasmalemmal Ca2PalytoxinATPaseCellsMaitotoxin
2004
Activation of Human TRPC6 Channels by Receptor Stimulation*
Estacion M, Li S, Sinkins W, Gosling M, Bahra P, Poll C, Westwick J, Schilling W. Activation of Human TRPC6 Channels by Receptor Stimulation*. Journal Of Biological Chemistry 2004, 279: 22047-22056. PMID: 15023993, DOI: 10.1074/jbc.m402320200.Peer-Reviewed Original ResearchMeSH KeywordsCalcium ChannelsCarbacholCell LineCell MembraneCholinergic AgonistsDNA, ComplementaryDose-Response Relationship, DrugElectrophysiologyEnzyme InhibitorsHumansIonomycinIonophoresKineticsLac OperonMembrane PotentialsProtein BindingTetradecanoylphorbol AcetateThapsigarginTime FactorsTransfectionTRPC Cation ChannelsTRPC6 Cation ChannelConceptsReceptor stimulationTRPC6 currentsTRPC6 channelsSteep dose-response relationshipEffect of carbacholConcentrations of carbacholSteep concentration-response relationshipReceptor-mediated eventsActivation of TRPC6Channel activityDose-response relationshipWhole-cell membrane currentsSignificant acute effectConcentration-response relationshipCell membrane currentsAcute effectsCarbacholGeneration of DAGHuman embryonic kidney cellsPrior stimulationPatch pipetteTRPC6 activityEmbryonic kidney cellsMembrane currentsMembrane depolarization
1998
Functional expression of TrpC1: a human homologue of the Drosophila Trp channel
SINKINS W, ESTACION M, SCHILLING W. Functional expression of TrpC1: a human homologue of the Drosophila Trp channel. Biochemical Journal 1998, 331: 331-339. PMID: 9512497, PMCID: PMC1219356, DOI: 10.1042/bj3310331.Peer-Reviewed Original ResearchConceptsStore-operated channelsPost-infection timesBasal cytosolic free Ca2Mammalian cellsExpression of TRPC1Whole-cell membrane currentsSf9 cellsNon-selective cation channelsCytosolic free Ca2Drosophila TRP channelsSf9 insect cellsInternal Ca2Baculovirus expression systemPlasmalemmal Ca2Basal Ba2TRPC1Free Ca2TRP channelsCation channelsGluconate solutionMembrane currentsInsect cellsAdditional subunitsHuman homologueCytoplasmic factors