2005
Identification and localization of TRPC channels in the rat kidney
Goel M, Sinkins W, Zuo C, Estacion M, Schilling W. Identification and localization of TRPC channels in the rat kidney. American Journal Of Physiology. Renal Physiology 2005, 290: f1241-f1252. PMID: 16303855, DOI: 10.1152/ajprenal.00376.2005.Peer-Reviewed Original ResearchConceptsTubular cellsDuct cellsTransient receptor potential channelsG protein-coupled membrane receptorsPresence of TRPC1Aquaporin-2Western blot analysisMesangial cell culturesKidney sectionsProximal tubulesOuter medullaRat kidneyTRPC channelsRenal nephronKidney lysateTRPC3Immunopositive labelingTRPC1PLC-dependent signalingRat kidney lysateInner medullaAffinity-purified antibodiesPrinciple cellsPolarized culturesCell lines
2004
Association of Immunophilins with Mammalian TRPC Channels*
Sinkins W, Goel M, Estacion M, Schilling W. Association of Immunophilins with Mammalian TRPC Channels*. Journal Of Biological Chemistry 2004, 279: 34521-34529. PMID: 15199065, DOI: 10.1074/jbc.m401156200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBaculoviridaeBrainCalcium ChannelsCell LineDose-Response Relationship, DrugElectrophysiologyGlutamineHumansImmunoblottingImmunophilinsInsectaMutationPeptidesPrecipitin TestsProlineProtein BindingProtein Structure, TertiaryRatsTacrolimus Binding Protein 1ATacrolimus Binding ProteinsTime FactorsTRPC Cation ChannelsConceptsTRPC channel proteinsChannel proteinsMammalian TRPC channelsTRPC channelsHEK cellsBinding of FKBP12Rat brain lysatesImmunosuppressant drug FK506Receptor-mediated activationSf9 cellsAccessory proteinsRat cerebral cortexImmunophilinsDrug FK506Brain lysatesFKBP52FKBP12Membrane lysatesGln mutationINADProteinFKBP59SignalplexTRPC1Cerebral cortex