2001
Regulation of Drosophila TRPL Channels by Immunophilin FKBP59*
Goel M, Garcia R, Estacion M, Schilling W. Regulation of Drosophila TRPL Channels by Immunophilin FKBP59*. Journal Of Biological Chemistry 2001, 276: 38762-38773. PMID: 11514552, DOI: 10.1074/jbc.m104125200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsAmino Acid SequenceAnimalsBinding SitesCalciumCalmodulin-Binding ProteinsCationsCell LineChelating AgentsDNA, ComplementaryDrosophilaDrosophila ProteinsEgtazic AcidElectrophysiologyGene LibraryGlutathione TransferaseHumansImmunohistochemistryInsectaMembrane ProteinsMicroscopy, FluorescenceMolecular Sequence DataMutationPhotoreceptor Cells, InvertebratePolymerase Chain ReactionPrecipitin TestsProtein BindingProtein Structure, TertiaryRecombinant ProteinsSequence Homology, Amino AcidSignal TransductionTacrolimus Binding ProteinsTime FactorsTransient Receptor Potential ChannelsTwo-Hybrid System TechniquesConceptsS2 cellsTRPL channelsDrosophila head cDNA libraryDrosophila photoreceptor cellsHuman FK506-binding proteinMultimeric signaling complexesTwo-hybrid screenDrosophila S2 cellsDrosophila TRPL channelsSf9 cell lysatesFK506-binding proteinChannel activitySite-directed mutagenesisCytoplasmic membrane surfaceFura-2 assayTransient receptor potentialDrosophila homologPermeable cation channelSignaling ComplexNovel proteinCDNA librarySf9 cellsINADCytoplasmic mouthChannel regulation
1999
Maitotoxin activates a nonselective cation channel and a P2Z/P2X7-like cytolytic pore in human skin fibroblasts
Schilling W, Sinkins W, Estacion M. Maitotoxin activates a nonselective cation channel and a P2Z/P2X7-like cytolytic pore in human skin fibroblasts. American Journal Of Physiology 1999, 277: c755-c765. PMID: 10516106, DOI: 10.1152/ajpcell.1999.277.4.c755.Peer-Reviewed Original ResearchConceptsEffects of maitotoxinConcentration-dependent increaseNonselective cation channelsCation channelsHuman skin fibroblastsPlasmalemmal permeabilitySkin fibroblastsUptake of ethidiumIntracellular fura-2Cytolytic poreYO-PRO-1Permeable cation channelFura-2Receptor stimulationTypes of cellsCytosolic freeLactate dehydrogenaseMembrane currentsPotent cytolytic agentCytolytic agentsMaitotoxinCell typesPresent studyCell levelSingle-cell level