1997
PDGF-Stimulated Calcium Influx Changes During In Vitro Cell Transformation
Estacion M, Mordan L. PDGF-Stimulated Calcium Influx Changes During In Vitro Cell Transformation. Cellular Signalling 1997, 9: 363-366. PMID: 9376215, DOI: 10.1016/s0898-6568(96)00184-2.Peer-Reviewed Original ResearchConceptsT-type calcium channelsCalcium signalsIntracellular calcium signalsC3H 10T1/2 mouse fibroblastsCalcium channelsPreneoplastic clonesMarked reductionGrowth factorMetabolism changesVitro Cell TransformationCell transformationColonies of cellsElectrophysiology measurementsInflux changeCellsMouse fibroblastsMutations Causing Achondroplasia and Thanatophoric Dysplasia Alter bFGF-Induced Calcium Signals in Human Diploid Fibroblasts
Nguyen H, Estacion M, Gargus J. Mutations Causing Achondroplasia and Thanatophoric Dysplasia Alter bFGF-Induced Calcium Signals in Human Diploid Fibroblasts. Human Molecular Genetics 1997, 6: 681-688. PMID: 9158142, DOI: 10.1093/hmg/6.5.681.Peer-Reviewed Original ResearchMeSH KeywordsAchondroplasiaBradykininCalciumCell LineDiploidyFibroblast Growth Factor 2FibroblastsHomozygoteHumansMutationProtein-Tyrosine KinasesReceptor Protein-Tyrosine KinasesReceptor, Fibroblast Growth Factor, Type 1Receptor, Fibroblast Growth Factor, Type 2Receptor, Fibroblast Growth Factor, Type 3Receptors, Fibroblast Growth FactorRNA SplicingSignal TransductionThanatophoric DysplasiaConceptsThanatophoric dysplasiaCalcium signalsBasic fibroblast growth factorCell linesCalcium-imaging analysesFibroblast growth factorFibroblast growth factor receptor (FGFR) gene familyHuman diploid fibroblastsPathophysiological mechanismsIntracellular calciumBlinded studyFGFR3 mutationsUnresponsive linesReceptor functionHereditary disorderTransient increaseGrowth factorDiploid fibroblastsControl cellsBone developmentFibroblast cell lineCalcium wavesReceptor gene familyHeterogeneous signalsAchondroplasia
1996
Calcium is permeable through a maitotoxin-activated nonselective cation channel in mouse L cells
Estacion M, Nguyen H, Gargus J. Calcium is permeable through a maitotoxin-activated nonselective cation channel in mouse L cells. American Journal Of Physiology 1996, 270: c1145-c1152. PMID: 8928742, DOI: 10.1152/ajpcell.1996.270.4.c1145.Peer-Reviewed Original ResearchConceptsNonselective cation channelsCation channelsCalcium-activated potassium channelsPlatelet-derived growth factorIntracellular calciumGrowth factor responseExtracellular calciumSecondary activationPotassium channelsGrowth factorFactor responseL cellsCell typesCalciumMouse L cellsL-cell fibroblastsMouse L-cell fibroblastsCell fibroblastsCellsIrreversible opening
1993
Ca2+ influx via T-type channels modulates PDGF-induced replication of mouse fibroblasts
Wang Z, Estacion M, Mordan L. Ca2+ influx via T-type channels modulates PDGF-induced replication of mouse fibroblasts. American Journal Of Physiology 1993, 265: c1239-c1246. PMID: 8238477, DOI: 10.1152/ajpcell.1993.265.5.c1239.Peer-Reviewed Original ResearchConceptsVoltage-gated calcium channelsExtracellular calcium influxCalcium influxNordihydroguariaretic acidCalcium currentWhole-cell patch-clamp techniqueCell patch-clamp techniqueIntracellular calcium increaseInward calcium currentCell proliferationIntracellular calcium concentrationT-type channelsAddition of prostaglandinPlatelet-derived growth factorMean inhibitory dosePatch-clamp techniqueC3H/10T1/2 mouse fibroblastsInhibitory doseCalcium channelsCalcium increaseSustained increaseGrowth factorInhibitory effectCalcium concentrationCell cycle progressionExpression of voltage-gated calcium channels correlates with PDGF-stimulated calcium influx and depends upon cell density in C3H 10T12 mouse fibroblasts
Estacion M, Mordan L. Expression of voltage-gated calcium channels correlates with PDGF-stimulated calcium influx and depends upon cell density in C3H 10T12 mouse fibroblasts. Cell Calcium 1993, 14: 161-171. PMID: 8384532, DOI: 10.1016/0143-4160(93)90085-k.Peer-Reviewed Original ResearchConceptsVoltage-gated calcium channelsCalcium channelsT-type voltage-gated calcium channelsWhole-cell patch-clamp recordingsWhole-cell patch-clamp techniqueCell patch-clamp techniquePatch-clamp recordingsPatch-clamp techniqueMembrane calcium channelsIntracellular calcium signalsPlasma membrane calcium channelsC3H 10T1/2 mouse fibroblastsCalcium currentCalcium influxFura-2Extracellular calciumCalcium poolSustained influxGrowth factorInflux componentFraction of cellsCalcium signalsCell densityMouse fibroblastsConfluent monolayers