2009
Maitotoxin converts the plasmalemmal Ca2+ pump into a Ca2+-permeable nonselective cation channel
Sinkins W, Estacion M, Prasad V, Goel M, Shull G, Kunze D, Schilling W. Maitotoxin converts the plasmalemmal Ca2+ pump into a Ca2+-permeable nonselective cation channel. American Journal Of Physiology - Cell Physiology 2009, 297: c1533-c1543. PMID: 19794142, PMCID: PMC2793065, DOI: 10.1152/ajpcell.00252.2009.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsAnimals, Genetically ModifiedCalciumCation Transport ProteinsCationsCell MembraneCells, CulturedDown-RegulationElectric ConductivityFibroblastsHumansIon ChannelsKidneyMarine ToxinsMiceOxocinsPermeabilityPlasma Membrane Calcium-Transporting ATPasesRNA, Small InterferingSpodopteraUp-RegulationConceptsPermeable nonselective cation channelNonselective cation channelsCation channelsSpodoptera frugiperda (Sf9) insect cellsHEK cellsMouse embryonic fibroblastsHuman embryonic kidney 293 cellsEmbryonic kidney 293 cellsKidney 293 cellsInsect cellsEmbryonic fibroblastsWhole-cell membrane currentsMolecular identityCell membrane currentsCell typesPMCACytosolic freeMarine toxinsEnhanced expressionWhole-cell currentsPlasmalemmal Ca2PalytoxinATPaseCellsMaitotoxin
2006
Palytoxin-induced cell death cascade in bovine aortic endothelial cells
Schilling W, Snyder D, Sinkins W, Estacion M. Palytoxin-induced cell death cascade in bovine aortic endothelial cells. American Journal Of Physiology - Cell Physiology 2006, 291: c657-c667. PMID: 16672692, DOI: 10.1152/ajpcell.00063.2006.Peer-Reviewed Original ResearchMeSH KeywordsAcrylamidesAnimalsAortaCalciumCattleCell DeathCells, CulturedCnidarian VenomsComputer SystemsDose-Response Relationship, DrugDrug Administration ScheduleEndothelial CellsEthidiumFluorescent DyesGreen Fluorescent ProteinsIntracellular MembranesMicroscopy, VideoOsmolar ConcentrationOuabainPertussis ToxinPropidiumConceptsAortic endothelial cellsBovine aortic endothelial cellsEndothelial cellsConcentration-dependent increaseCell deathAddition of ouabainMonovalent cation channelCell death cascadeCell lysisMarine toxin palytoxinCation channelsOuabainOncotic cell deathEB uptakeATPase pumpDeath cascadeTime-lapse video microscopyPropidium iodideDeathYO-PRO-1ExtracellularEthidium bromideDownstream eventsRapid uptake
2004
Maitotoxin Induces Biphasic Interleukin-1β Secretion and Membrane Blebbing in Murine Macrophages
Verhoef P, Kertesy S, Estacion M, Schilling W, Dubyak G. Maitotoxin Induces Biphasic Interleukin-1β Secretion and Membrane Blebbing in Murine Macrophages. Molecular Pharmacology 2004, 66: 909-920. PMID: 15385641, DOI: 10.1124/mol.66.4.909.Peer-Reviewed Original ResearchConceptsMTX treatmentMIL-1betaIL-1betaCytolytic releaseProIL-1betaProinflammatory cytokine interleukin-1betaProinflammatory cytokine secretionCytokine interleukin-1betaMature IL-1betaMurine macrophagesInterleukin-1β secretionCytokine releaseInflammatory leukocytesCytokine secretionP2X7 receptorInterleukin-1betaInflammatory macrophagesExtracellular Ca2Extracellular glycineIntracellular Ca2Vivo responseMacrophagesCation channelsSecretionLytic phaseMaitotoxin-induced cell death cascade in bovine aortic endothelial cells: divalent cation specificity and selectivity
Wisnoskey B, Estacion M, Schilling W. Maitotoxin-induced cell death cascade in bovine aortic endothelial cells: divalent cation specificity and selectivity. American Journal Of Physiology - Cell Physiology 2004, 287: c345-c356. PMID: 15044153, DOI: 10.1152/ajpcell.00473.2003.Peer-Reviewed Original ResearchConceptsCell death cascadeSingle-cell levelBovine aortic endothelial cellsDeath cascadeMembrane blebsAortic endothelial cellsCell population levelGreen fluorescent proteinSubsequent cell lysisPlasmalemmal permeabilityCell demiseEndothelial cellsFluorescent proteinLarge proteinsCation specificityNonselective cation channelsProteinCation channelsLytic phaseCytosolic freeCell lysisPropidium iodidePopulation levelEthidium bromideCascade
1999
Maitotoxin activates a nonselective cation channel and a P2Z/P2X7-like cytolytic pore in human skin fibroblasts
Schilling W, Sinkins W, Estacion M. Maitotoxin activates a nonselective cation channel and a P2Z/P2X7-like cytolytic pore in human skin fibroblasts. American Journal Of Physiology 1999, 277: c755-c765. PMID: 10516106, DOI: 10.1152/ajpcell.1999.277.4.c755.Peer-Reviewed Original ResearchConceptsEffects of maitotoxinConcentration-dependent increaseNonselective cation channelsCation channelsHuman skin fibroblastsPlasmalemmal permeabilitySkin fibroblastsUptake of ethidiumIntracellular fura-2Cytolytic poreYO-PRO-1Permeable cation channelFura-2Receptor stimulationTypes of cellsCytosolic freeLactate dehydrogenaseMembrane currentsPotent cytolytic agentCytolytic agentsMaitotoxinCell typesPresent studyCell levelSingle-cell levelStimulation of Drosophila TrpL by capacitative Ca2+ entry.
Estacion M, Sinkins W, Schilling W. Stimulation of Drosophila TrpL by capacitative Ca2+ entry. Biochemical Journal 1999, 341 ( Pt 1): 41-9. PMID: 10377243, PMCID: PMC1220328, DOI: 10.1042/0264-6021:3410041.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBariumBiological TransportCalciumCalcium-Transporting ATPasesCalmodulinCalmodulin-Binding ProteinsCations, DivalentDrosophilaDrosophila ProteinsEndoplasmic ReticulumHumansIon Channel GatingIon ChannelsLanthanumMembrane ProteinsPatch-Clamp TechniquesRecombinant Fusion ProteinsSpodopteraStrontiumThapsigarginTransient Receptor Potential ChannelsConceptsCapacitative Ca2Internal Ca2Receptor-activated channelsCell-attached patch recordingsTRP-like proteinsNon-selective cation channelsCytosolic free Ca2CBS-2Fura-2Cation entryPatch recordingsHuman TRPC1Single-channel activityDrosophila TRPLTRPL activityFree Ca2Dependent mechanismCation influxGel overlay experimentsCation channelsPhotoreceptor cellsChannel activityPhospholipase CTRPC1TRPL channel activityStimulation of Drosophila TrpL by capacitative Ca2+ entry
ESTACION M, SINKINS W, SCHILLING W. Stimulation of Drosophila TrpL by capacitative Ca2+ entry. Biochemical Journal 1999, 341: 41-49. DOI: 10.1042/bj3410041.Peer-Reviewed Original ResearchCapacitative Ca2Internal Ca2Receptor-activated channelsCell-attached patch recordingsTRP-like proteinsNon-selective cation channelsCytosolic free Ca2CBS-2Fura-2Cation entryPatch recordingsHuman TRPC1Single-channel activityDrosophila TRPLTRPL activityFree Ca2Dependent mechanismCation influxGel overlay experimentsCation channelsPhotoreceptor cellsChannel activityPhospholipase CTRPC1TRPL channel activity
1998
Functional expression of TrpC1: a human homologue of the Drosophila Trp channel
SINKINS W, ESTACION M, SCHILLING W. Functional expression of TrpC1: a human homologue of the Drosophila Trp channel. Biochemical Journal 1998, 331: 331-339. PMID: 9512497, PMCID: PMC1219356, DOI: 10.1042/bj3310331.Peer-Reviewed Original ResearchConceptsStore-operated channelsPost-infection timesBasal cytosolic free Ca2Mammalian cellsExpression of TRPC1Whole-cell membrane currentsSf9 cellsNon-selective cation channelsCytosolic free Ca2Drosophila TRP channelsSf9 insect cellsInternal Ca2Baculovirus expression systemPlasmalemmal Ca2Basal Ba2TRPC1Free Ca2TRP channelsCation channelsGluconate solutionMembrane currentsInsect cellsAdditional subunitsHuman homologueCytoplasmic factors
1996
Calcium is permeable through a maitotoxin-activated nonselective cation channel in mouse L cells
Estacion M, Nguyen H, Gargus J. Calcium is permeable through a maitotoxin-activated nonselective cation channel in mouse L cells. American Journal Of Physiology 1996, 270: c1145-c1152. PMID: 8928742, DOI: 10.1152/ajpcell.1996.270.4.c1145.Peer-Reviewed Original ResearchConceptsNonselective cation channelsCation channelsCalcium-activated potassium channelsPlatelet-derived growth factorIntracellular calciumGrowth factor responseExtracellular calciumSecondary activationPotassium channelsGrowth factorFactor responseL cellsCell typesCalciumMouse L cellsL-cell fibroblastsMouse L-cell fibroblastsCell fibroblastsCellsIrreversible opening
1991
Acute electrophysiological responses of bradykinin‐stimulated human fibroblasts.
Estacion M. Acute electrophysiological responses of bradykinin‐stimulated human fibroblasts. The Journal Of Physiology 1991, 436: 603-620. PMID: 1648132, PMCID: PMC1181524, DOI: 10.1113/jphysiol.1991.sp018569.Peer-Reviewed Original ResearchConceptsFura-2 fluorescencePipette solutionIon substitution experimentsGTP gamma SReversal potentialBradykinin stimulationPresence of GTPHuman dermal fibroblastsGamma SPatch-clamp techniqueNon-specific cation channelsBradykinin additionDermal fibroblastsIntracellular calciumLittle voltage dependencePatch-clamped cellsTail current analysisAcute responseInward currentsCalcium ionophoreOutward currentsNear baselineCation channelsElectrophysiological responsesBradykinin