2001
Ductular morphogenesis and functional polarization of normal human biliary epithelial cells in three-dimensional culture
Ishida Y, Smith S, Wallace L, Sadamoto T, Okamoto M, Auth M, Strazzabosco M, Fabris L, Medina J, Prieto J, Strain A, Neuberger J, Joplin R. Ductular morphogenesis and functional polarization of normal human biliary epithelial cells in three-dimensional culture. Journal Of Hepatology 2001, 35: 2-9. PMID: 11495037, DOI: 10.1016/s0168-8278(01)00078-2.Peer-Reviewed Original ResearchConceptsCollagen gel cultureHuman biliary epithelial cellsEpithelial cellsGel cultureGrowth factorHuman hepatocyte growth factorThree-dimensional cultureBiliary epithelial cellsThree-dimensional aggregatesNormal human biliary epithelial cellsFoetal bovine serumMorphogenesisCell typesAnion exchanger 2Hepatocyte growth factorFunctional polarizationFunctional differentiationFunctional markersExchanger 2Monolayer culture systemCentral lumenPhenotypic markersCollagen gelsNumber of aggregatesCulture system
1998
Functional polarity of Na+/H+and Cl−/ HCO 3 − exchangers in a rat cholangiocyte cell line
Spirlì C, Granato A, Zsembery A, Anglani F, Okolicsànyi L, LaRusso N, Crepaldi G, Strazzabosco M. Functional polarity of Na+/H+and Cl−/ HCO 3 − exchangers in a rat cholangiocyte cell line. American Journal Of Physiology 1998, 275: g1236-g1245. PMID: 9843758, DOI: 10.1152/ajpgi.1998.275.6.g1236.Peer-Reviewed Original ResearchConceptsRat cholangiocyte cell lineCholangiocyte cell lineHOE 642Intrahepatic bile duct cellsCell linesNHE isoformsBile duct cellsAcid-base transportersPHi recoveryApical administrationBasolateral amilorideSeparate perfusionFirst functional descriptionAcid loadBasolateral aspectNHE3 gene expressionDuct cellsGene expressionRT-PCRApical amilorideElectrolyte transportAE2 mRNAAmilorideHCO-3Isoforms