1992
A Rapid Quantitative Bioassay for Evaluating the Effects of Ligands Upon Receptors That Modulate cAMP Levels in a Melanophore Cell Line
POTENZA M, LERNER M. A Rapid Quantitative Bioassay for Evaluating the Effects of Ligands Upon Receptors That Modulate cAMP Levels in a Melanophore Cell Line. Pigment Cell & Melanoma Research 1992, 5: 372-378. PMID: 1337205, DOI: 10.1111/j.1600-0749.1992.tb00565.x.Peer-Reviewed Original ResearchConceptsDose-dependent pigment dispersionCell linesBeta-adrenergic receptorsEffects of antagonistsMelanophore cell lineEffects of drugsDose-response curveReproducible dose-response curvesAdrenergic agonistsAdrenergic receptorsIntracellular cAMPCAMP levelsReceptorsSubsequent exposureTime-course analysisXenopus laevis melanophoresA method for evaluating the effects of ligands upon Gs protein-coupled receptors using a recombinant melanophore-based bioassay
Potenza M, Graminski G, Lerner M. A method for evaluating the effects of ligands upon Gs protein-coupled receptors using a recombinant melanophore-based bioassay. Analytical Biochemistry 1992, 206: 315-322. PMID: 1332547, DOI: 10.1016/0003-2697(92)90372-e.Peer-Reviewed Original ResearchConceptsBeta-2 adrenergic receptorsAdrenergic receptorsGs protein-coupled receptorsEffects of drugsDose-response curveHuman beta 2 adrenergic receptorRank order potencyProtein-coupled receptorsReproducible dose-response curvesPharmacological characterizationConcomitant elevationStimulatory guaninePigment dispersionAgonistsIntracellular cAMPImportant receptorReceptorsAntagonistSpecific GsIC50 valuesFunctional expressionCellsXenopus laevis
1991
A Recombinant Vaccinia Virus Infects Xenopus Melanophores
Potenza M, Lerner M. A Recombinant Vaccinia Virus Infects Xenopus Melanophores. Pigment Cell & Melanoma Research 1991, 4: 186-192. PMID: 1816550, DOI: 10.1111/j.1600-0749.1991.tb00437.x.Peer-Reviewed Original ResearchConceptsXenopus cellsWestern blot analysisXenopus melanophoresMultiplicity of infectionCultured Xenopus laevis melanophoresHuman growth hormone geneBlot analysisGrowth hormone geneVirus/host interactionsBeta-galactosidase productionTranscriptional controlAmphibian cellsXenopus laevis melanophoresAmplification of virusPigment cellsLate proteinsViral promotersEfficient expressionHormone geneHost interactionsVaccinia virusLac ZEfficient replication