1993
A New Tool for Investigating G Protein‐Coupled Receptors
Lerner M, Potenza M, Graminski G, Mcclintock T, Jayawickreme C, Karne S. A New Tool for Investigating G Protein‐Coupled Receptors. Novartis Foundation Symposia 1993, 179: 76-96. PMID: 8168384, DOI: 10.1002/9780470514511.ch6.Peer-Reviewed Original ResearchMeSH KeywordsAdenylyl CyclasesAnimalsEnzyme ActivationGTP-Binding ProteinsReceptors, OdorantRecombinant ProteinsType C PhospholipasesConceptsG protein-coupled receptorsProtein-coupled receptorsIntracellular signal transduction pathwaysVertebrate olfactory receptorsSignal transduction pathwaysG protein-coupled receptor familyStructure-function analysisProtein-coupled receptor familyCloning of cDNASecond messenger cAMPOdorant receptorsTransduction pathwaysG proteinsOlfactory receptorsFunctional interactionReceptor familyReceptor couplingReceptorsCloningCDNANew toolProteinDiacylglycerolTrisphosphateInositol
1992
A method for evaluating the effects of ligands upon Gs protein-coupled receptors using a recombinant melanophore-based bioassay
Potenza M, Graminski G, Lerner M. A method for evaluating the effects of ligands upon Gs protein-coupled receptors using a recombinant melanophore-based bioassay. Analytical Biochemistry 1992, 206: 315-322. PMID: 1332547, DOI: 10.1016/0003-2697(92)90372-e.Peer-Reviewed Original ResearchConceptsBeta-2 adrenergic receptorsAdrenergic receptorsGs protein-coupled receptorsEffects of drugsDose-response curveHuman beta 2 adrenergic receptorRank order potencyProtein-coupled receptorsReproducible dose-response curvesPharmacological characterizationConcomitant elevationStimulatory guaninePigment dispersionAgonistsIntracellular cAMPImportant receptorReceptorsAntagonistSpecific GsIC50 valuesFunctional expressionCellsXenopus laevis