2024
Applications of cell free protein synthesis in protein design
Thornton E, Paterson S, Stam M, Wood C, Laohakunakorn N, Regan L. Applications of cell free protein synthesis in protein design. Protein Science 2024, 33: e5148. PMID: 39180484, PMCID: PMC11344276, DOI: 10.1002/pro.5148.Peer-Reviewed Original Research
2009
Redesign of a protein–peptide interaction: Characterization and applications
Jackrel ME, Valverde R, Regan L. Redesign of a protein–peptide interaction: Characterization and applications. Protein Science 2009, 18: 762-774. PMID: 19309728, PMCID: PMC2762588, DOI: 10.1002/pro.75.Peer-Reviewed Original ResearchConceptsProtein-peptide interactionsProtein-peptide pairsPurification applicationsRecognition pairsCorresponding peptide ligandsRepeat protein scaffoldsMolecular recognitionAntibody-antigen interactionsFacile replacementChemical considerationsProtein scaffoldsPeptide ligandsTypical antibody-antigen interactionsHarsh conditionsProtein designModerate affinityScaffoldsPractical applicationsAffinity purificationAffinityLigandsPropertiesInteractionSuccessful applicationDissociation