Redesign of a protein–peptide interaction: Characterization and applications
Jackrel ME, Valverde R, Regan L. Redesign of a protein–peptide interaction: Characterization and applications. Protein Science 2009, 18: 762-774. PMID: 19309728, PMCID: PMC2762588, DOI: 10.1002/pro.75.Peer-Reviewed Original ResearchConceptsProtein-peptide interactionsProtein-peptide pairsPurification applicationsRecognition pairsCorresponding peptide ligandsRepeat protein scaffoldsMolecular recognitionAntibody-antigen interactionsFacile replacementChemical considerationsProtein scaffoldsPeptide ligandsTypical antibody-antigen interactionsHarsh conditionsProtein designModerate affinityScaffoldsPractical applicationsAffinity purificationAffinityLigandsPropertiesInteractionSuccessful applicationDissociation