2023
Evolutionarily conserved midbody remodeling precedes ring canal formation during gametogenesis
Price K, Tharakan D, Cooley L. Evolutionarily conserved midbody remodeling precedes ring canal formation during gametogenesis. Developmental Cell 2023, 58: 474-488.e5. PMID: 36898376, PMCID: PMC10059090, DOI: 10.1016/j.devcel.2023.02.008.Peer-Reviewed Original ResearchConceptsCanal formationStable intercellular bridgesGerm cell divisionMidbody ringTime-lapse imagingFemale germlineCell cytokinesisDrosophila malesRing canalsComplete cytokinesisKinase functionCell divisionCytokinesis eventsBroad functionsCytokinesisIntercellular bridgesExtensive remodelingMidbodyDrosophilaBiological systemsDisease statesImportant insightsGametogenesisGermlineProtein
2019
Proximity labeling reveals novel interactomes in live Drosophila tissue
Mannix KM, Starble RM, Kaufman RS, Cooley L. Proximity labeling reveals novel interactomes in live Drosophila tissue. Development 2019, 146: dev176644. PMID: 31208963, PMCID: PMC6679357, DOI: 10.1242/dev.176644.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActinsAnimalsAnimals, Genetically ModifiedCell CommunicationCell DifferentiationCytological TechniquesCytoskeletonDNA-(Apurinic or Apyrimidinic Site) LyaseDrosophila melanogasterFemaleGenes, ReporterGerm CellsIntercellular JunctionsMolecular ImagingOocytesOogenesisProtein BindingProtein Interaction MapsStaining and LabelingConceptsProximity labelingIntercellular bridgesProximity-dependent biotinylationStable intercellular bridgesRC proteinDynamic actin cytoskeletonProtein interactome analysisRNA interference screenNovel interactomePrey genesUncharacterized proteinsDistinct interactomesDrosophila tissuesActin cytoskeletonInterference screenInteractome analysisLive tissueMultiple proteinsProximity ligationInteractomeGerm cellsIntercellular communicationRespective preyFunctional roleProtein
2014
Somatic insulin signaling regulates a germline starvation response in Drosophila egg chambers
Burn KM, Shimada Y, Ayers K, Vemuganti S, Lu F, Hudson A, Cooley L. Somatic insulin signaling regulates a germline starvation response in Drosophila egg chambers. Developmental Biology 2014, 398: 206-217. PMID: 25481758, PMCID: PMC4340711, DOI: 10.1016/j.ydbio.2014.11.021.Peer-Reviewed Original ResearchConceptsDrosophila insulin-like peptidesEgg chambersStarvation responseBody organizationDrosophila egg chamberMotor protein dyneinNutrient-rich conditionsPoor nutrient availabilityInsulin-like peptidesProcessing bodiesDrosophila femalesGermline cellsP-bodiesNutrient availabilityDynein activityInsulin signalingProgeny survivalInsulin pathwayKinesin activityFollicle cellsMicrotubulesStarvationBovine insulinPotential mechanismsProtective responseA Regulatory Network of Drosophila Germline Stem Cell Self-Renewal
Yan D, Neumüller RA, Buckner M, Ayers K, Li H, Hu Y, Yang-Zhou D, Pan L, Wang X, Kelley C, Vinayagam A, Binari R, Randklev S, Perkins LA, Xie T, Cooley L, Perrimon N. A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal. Developmental Cell 2014, 28: 459-473. PMID: 24576427, PMCID: PMC3998650, DOI: 10.1016/j.devcel.2014.01.020.Peer-Reviewed Original ResearchConceptsGermline stem cellsSelf-renewal factorsDrosophila female germline stem cellsStem cellsDifferent stem cell lineagesLarge-scale RNAi screenFemale germline stem cellsLoss of Set1Stem Cell Self-RenewalSpecific genetic networksHistone methyltransferase Set1Stem cell identityCell fate decisionsStem cell lineagesCell Self-RenewalSelf-renewal genesRNAi screenDistinct fatesFate decisionsGSC maintenanceCell identityCell fateRegulatory networksGenetic networksNeural stem cells
2001
Comparative Aspects of Animal Oogenesis
Matova N, Cooley L. Comparative Aspects of Animal Oogenesis. Developmental Biology 2001, 231: 291-320. PMID: 11237461, DOI: 10.1006/dbio.2000.0120.Peer-Reviewed Original Research
1993
Kelch encodes a component of intercellular bridges in Drosophila egg chambers
Xue F, Cooley L. Kelch encodes a component of intercellular bridges in Drosophila egg chambers. Cell 1993, 72: 681-693. PMID: 8453663, DOI: 10.1016/0092-8674(93)90397-9.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBiological TransportCarrier ProteinsConserved SequenceDrosophilaDrosophila ProteinsGerm CellsIntercellular JunctionsMicrofilament ProteinsMolecular Sequence DataOogenesisOpen Reading FramesRepetitive Sequences, Nucleic AcidSequence Homology, Amino AcidConceptsRing canalsIntercellular bridgesFemale-sterile mutationsDrosophila egg chamberUGA stop codonOpen reading frameFlow of cytoplasmSterile mutationsEgg chambersLong proteinShorter proteinCytoplasm transportORF1 productUGA codonReading frameKelch geneUnusual transcriptsNurse cellsProtein productsStop codonKelchOocyte maturationCodonORF1Cytoplasm