2012
Expression of Ixodes scapularis Antifreeze Glycoprotein Enhances Cold Tolerance in Drosophila melanogaster
Neelakanta G, Hudson AM, Sultana H, Cooley L, Fikrig E. Expression of Ixodes scapularis Antifreeze Glycoprotein Enhances Cold Tolerance in Drosophila melanogaster. PLOS ONE 2012, 7: e33447. PMID: 22428051, PMCID: PMC3302814, DOI: 10.1371/journal.pone.0033447.Peer-Reviewed Original ResearchMeSH KeywordsAcclimatizationAnalysis of VarianceAnimalsAnimals, Genetically ModifiedAntifreeze ProteinsApoptosisCold TemperatureDrosophila melanogasterEmbryo, NonmammalianEnzyme-Linked Immunosorbent AssayFemaleImmunoblottingIn Situ Nick-End LabelingIxodesMaleMusclesOligonucleotidesReal-Time Polymerase Chain ReactionConceptsNon-freezing temperaturesD. melanogasterDrosophila melanogasterCold toleranceLow non-freezing temperaturesFemale adult fliesTransgenic D. melanogasterCold shock injuryAbility of fliesAntifreeze glycoproteinsAdult fliesMolecular basisMelanogasterFlight musclesFliesAntifreeze proteinsHatching rateHigher survival rateApoptotic damageGlycoproteinExpressionToleranceEmbryosProteinApoptosis
2004
Flytrap, a database documenting a GFP protein‐trap insertion screen in Drosophila melanogaster
Kelso RJ, Buszczak M, Quiñones AT, Castiblanco C, Mazzalupo S, Cooley L. Flytrap, a database documenting a GFP protein‐trap insertion screen in Drosophila melanogaster. Nucleic Acids Research 2004, 32: d418-d420. PMID: 14681446, PMCID: PMC308749, DOI: 10.1093/nar/gkh014.Peer-Reviewed Original ResearchConceptsProtein localizationInsertion linesDrosophila melanogasterGFP fusion proteinTransposable element insertionsCellular component ontologyGene Ontology ConsortiumTransposable P elementsInverse PCR productsGene intronsArtificial exonElement insertionsGenomic DNAFusion proteinFlytrapMelanogasterComponent ontologyP elementsStages of developmentType of tissueLocalizationFlyBaseIntronsExonsGenes
1999
Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila
Buszczak M, Freeman M, Carlson J, Bender M, Cooley L, Segraves W. Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila. Development 1999, 126: 4581-4589. PMID: 10498692, DOI: 10.1242/dev.126.20.4581.Peer-Reviewed Original ResearchConceptsEgg chamber developmentGermline clonesResponse genesGene expressionDorsal follicle cell fatesEgg chamber maturationMutant egg chambersFollicle cell fatesSteroid hormone ecdysoneEcdysone response genesProgression of oogenesisTemperature-sensitive mutationStage-specific mannerTemporal expression profilesEarly response genesChamber developmentEcdysone responsivenessChamber maturationHormone ecdysoneGenetic hierarchyEcR expressionDrosophila melanogasterEgg chambersCell fateEarly gene expression
1983
Organization and Expression of tRNA Genes in Drosophila Melanogaster
Sharp S, Cooley L, DeFranco D, Dingermann T, Söll D. Organization and Expression of tRNA Genes in Drosophila Melanogaster. Recent Results In Cancer Research 1983, 84: 1-14. PMID: 6405456, DOI: 10.1007/978-3-642-81947-6_1.Peer-Reviewed Original ResearchConceptsProtein-synthesizing machineryCorrect amino acidCognate aminoacyl-tRNA synthetasesAminoacyl-tRNA synthetasesTransfer RNA moleculesSpecific recognition featuresStructure-function relationshipsTRNA genesDrosophila melanogasterTRNA speciesRNA moleculesGeneral structural featuresPolypeptide chainTRNAAmino acidsRegulatory processesUseful moleculesFunctional characteristicsStructural featuresIntegral componentRecognition featuresMelanogasterEfficient recognitionSynthetasesRibosomes
1982
Post-transcriptional nucleotide addition is responsible for the formation of the 5' terminus of histidine tRNA.
Cooley L, Appel B, Söll D. Post-transcriptional nucleotide addition is responsible for the formation of the 5' terminus of histidine tRNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1982, 79: 6475-6479. PMID: 6292903, PMCID: PMC347149, DOI: 10.1073/pnas.79.21.6475.Peer-Reviewed Original ResearchConceptsMature tRNAHistidine tRNAPrimary transcriptHistidine tRNA genesGuanylate residuePost-transcriptional additionDrosophila Kc cellsTRNA genesDrosophila melanogasterSchizosaccharomyces pombeTRNAs resultsRNA speciesRNase PEukaryotic mRNAsKc cellsRNA precursorsTRNASequence analysisNucleotide additionAdditional nucleotidesPhosphodiester bondGenesNucleotidesMaturation schemeTranscripts