2020
HtsRC-Mediated Accumulation of F-Actin Regulates Ring Canal Size During Drosophila melanogaster Oogenesis
Gerdes JA, Mannix KM, Hudson AM, Cooley L. HtsRC-Mediated Accumulation of F-Actin Regulates Ring Canal Size During Drosophila melanogaster Oogenesis. Genetics 2020, 216: 717-734. PMID: 32883702, PMCID: PMC7648574, DOI: 10.1534/genetics.120.303629.Peer-Reviewed Original ResearchConceptsGermline ring canalsRing canalsActin cytoskeletonF-actinDrosophila melanogaster oogenesisSomatic follicle cellsCombination of CRISPRF-actin accumulationF-actin recruitmentFilamentous actin cytoskeletonFemale germlineActin structuresFruit flyHigh fecundityFollicle cellsCytoskeletonGermlineOverexpressionAccumulationDrosophilaOogenesisMutagenesisCRISPRFilaminGenes
2018
Targeted substrate degradation by Kelch controls the actin cytoskeleton during ring canal expansion
Hudson AM, Mannix KM, Gerdes JA, Kottemann MC, Cooley L. Targeted substrate degradation by Kelch controls the actin cytoskeleton during ring canal expansion. Development 2018, 146: dev169219. PMID: 30559276, PMCID: PMC6340150, DOI: 10.1242/dev.169219.Peer-Reviewed Original ResearchConceptsTandem affinity purificationUbiquitin ligase complexCullin-3 functionShort sequence motifsSpecialized cytoskeletal structuresUbiquitin-proteasome systemF-actin cytoskeletonSpecialized actinLigase complexActin cytoskeletonRing canalsSequence motifsGenetic evidenceCytoskeletal structuresAffinity purificationCytoskeletonSubstrate degradationBiochemical evidenceUnusual mechanismKelchCRL3CullinMass spectrometryOogenesisMutagenesis
2015
Actin Cytoskeletal Organization in Drosophila Germline Ring Canals Depends on Kelch Function in a Cullin-RING E3 Ligase
Hudson AM, Mannix KM, Cooley L. Actin Cytoskeletal Organization in Drosophila Germline Ring Canals Depends on Kelch Function in a Cullin-RING E3 Ligase. Genetics 2015, 201: 1117-1131. PMID: 26384358, PMCID: PMC4649639, DOI: 10.1534/genetics.115.181289.Peer-Reviewed Original ResearchConceptsKelch functionE3 ligaseCullin-RING E3 ligaseGermline ring canalsActin cytoskeletal organizationDrosophila kelch proteinUbiquitin ligase activityCross-link F-actinUbiquitin E3 ligaseRing canalsKelch proteinProtein substratesCytoskeletal defectsCytoskeletal organizationCytoskeletal remodelingLigase activityCullin 3KelchF-actinCytoskeletonLigaseProteasomeVivoCul3Mutagenesis
2010
Drosophila Kelch functions with Cullin-3 to organize the ring canal actin cytoskeleton
Hudson AM, Cooley L. Drosophila Kelch functions with Cullin-3 to organize the ring canal actin cytoskeleton. Journal Of Cell Biology 2010, 188: 29-37. PMID: 20065088, PMCID: PMC2812842, DOI: 10.1083/jcb.200909017.Peer-Reviewed Original ResearchConceptsDrosophila KelchCullin 3Cullin-RING ubiquitin E3 ligasesGermline ring canalsSubstrate adaptor proteinCullin-RING ligaseDiverse protein familiesF-actin cytoskeletal structureUbiquitin E3 ligasesProtein ubiquitylationActin cytoskeletonE3 ligasesRing canalsAdaptor proteinProtein familySequence motifsCytoskeletal structuresFilamentous actinKelchProteinUbiquitylationLigasesCytoskeletonLigaseRepeats
2002
UNDERSTANDING THE FUNCTION OF ACTIN-BINDING PROTEINS THROUGH GENETIC ANALYSIS OF DROSOPHILA OOGENESIS
Hudson AM, Cooley L. UNDERSTANDING THE FUNCTION OF ACTIN-BINDING PROTEINS THROUGH GENETIC ANALYSIS OF DROSOPHILA OOGENESIS. Annual Review Of Genetics 2002, 36: 455-488. PMID: 12429700, DOI: 10.1146/annurev.genet.36.052802.114101.Peer-Reviewed Original ResearchConceptsActin-binding proteinsActin cytoskeletonGenetic analysisNew actin-binding proteinCell biological approachesGenetic model systemActin binding proteinsRecent genetic analysesDrosophila ovaryDrosophila oogenesisGenetic screenBiological approachesGenetic resultsProteinCytoskeletonOogenesisModel systemUltrastructural characteristicsActinScreenUnderstandingOvaries
1997
GENETIC ANALYSIS OF THE ACTIN CYTOSKELETON IN THE DROSOPHILA OVARY
Robinson D, Cooley L. GENETIC ANALYSIS OF THE ACTIN CYTOSKELETON IN THE DROSOPHILA OVARY. Annual Review Of Cell And Developmental Biology 1997, 13: 147-170. PMID: 9442871, DOI: 10.1146/annurev.cellbio.13.1.147.Peer-Reviewed Original ResearchConceptsDrosophila ovaryActin cytoskeletonStable intercellular bridgesSpecific subcellular localizationCell shape changesCell biological studiesFavorable model systemCellular morphogenesisGermline cellsSubcellular localizationIntercellular transportDynamic cytoskeletonDrosophila eggsGenetic analysisRecent geneticFollicle cellsIntercellular bridgesCytoskeletonCell migrationEgg developmentMature eggsMorphogenesisModel systemBiological studiesShape changesDrosophila Kelch Is an Oligomeric Ring Canal Actin Organizer
Robinson D, Cooley L. Drosophila Kelch Is an Oligomeric Ring Canal Actin Organizer. Journal Of Cell Biology 1997, 138: 799-810. PMID: 9265647, PMCID: PMC2138045, DOI: 10.1083/jcb.138.4.799.Peer-Reviewed Original ResearchConceptsDrosophila KelchRing canalsAmino halfKelch repeat domainStructure-function analysisAmino-terminal regionGerm cell membranesKelch family proteinDominant sterilityBTB domainProtein domainsRepeat domainKelchActin filamentsCell membraneProteinCanal localizationAdditional interactionsDrosophilaDomainCytoskeletonOogenesisLocalizationSterilityActinFormation of the Drosophila Ovarian Ring Canal Inner Rim Depends on cheerio
Robinson D, Smith-Leiker T, Sokol N, Hudson A, Cooley L. Formation of the Drosophila Ovarian Ring Canal Inner Rim Depends on cheerio. Genetics 1997, 145: 1063-1072. PMID: 9093858, PMCID: PMC1207876, DOI: 10.1093/genetics/145.4.1063.Peer-Reviewed Original ResearchMeSH KeywordsActinsAllelesAnimalsCalmodulin-Binding ProteinsCarrier ProteinsCell CommunicationCell MembraneChromosome MappingCytoskeletonDrosophila melanogasterDrosophila ProteinsFemaleGene Expression Regulation, DevelopmentalGenes, InsectInfertility, FemaleInsect ProteinsIntercellular JunctionsMicrofilament ProteinsOocytesOvaryConceptsStable intercellular bridgesExamination of mutantsDrosophila oogenesisPlasma membrane stabilizationRing canalsCytoplasm transportMutant cellsFilamentous actinCleavage furrowRIM proteinsNurse cellsActin filamentsIntercellular bridgesMutantsCritical functionsKelchCheeriosProteinStep-wise processAssemblyMembrane stabilizationCellsCytoskeletonOogenesisGenes
1996
Stable intercellular bridges in development: the cytoskeleton lining the tunnel
Robinson D, Cooley L. Stable intercellular bridges in development: the cytoskeleton lining the tunnel. Trends In Cell Biology 1996, 6: 474-479. PMID: 15157506, DOI: 10.1016/0962-8924(96)84945-2.Peer-Reviewed Original ResearchStable intercellular bridgesIntercellular bridgesIntercellular junctionsExamination of mutantsDrosophila germlineRing canalsSignal transductionIntercellular transportCytoplasmic connectionsCell adhesionAssemblyCytoskeletonMutantsOrganellesGermlineTransductionOrganismsCytoplasmWide varietyCellsRapid transferAdhesionJunction
1994
Cytoskeletal Functions During Drosophila Oogenesis
Cooley L, Theurkauf W. Cytoskeletal Functions During Drosophila Oogenesis. Science 1994, 266: 590-596. PMID: 7939713, DOI: 10.1126/science.7939713.Peer-Reviewed Original ResearchConceptsDrosophila oogenesisCytoskeletal functionMature Drosophila oocytesOrganismal morphogenesisDrosophila oocytesCytoskeletal organizationCytoskeletal transformationCell shapeCytoskeletal elementsOogenesisCytological studiesSpecific functionsCell morphologyComplex seriesMechanistic implicationsMechanisms of developmentExperimental approachBasic cytoarchitectureCytoskeletonVersatile systemMorphogenesisCytoplasmOocytesFunctionCellsThe specialized cytoskeleton of theDrosophila egg chamber
Knowles B, Cooley L. The specialized cytoskeleton of theDrosophila egg chamber. Trends In Genetics 1994, 10: 235-241. PMID: 8091503, DOI: 10.1016/0168-9525(94)90170-8.Peer-Reviewed Original Research