2020
Structures of the β‐barrel assembly machine recognizing outer membrane protein substrates
Xiao L, Han L, Li B, Zhang M, Zhou H, Luo Q, Zhang X, Huang Y. Structures of the β‐barrel assembly machine recognizing outer membrane protein substrates. The FASEB Journal 2020, 35: e21207. PMID: 33368572, DOI: 10.1096/fj.202001443rr.Peer-Reviewed Original ResearchConceptsBAM complexFirst β-strandOMP substratesΒ-strandsΒ-barrel outer membrane proteinsΒ-barrel assembly machinery (BAM) complexΒ-barrel assembly machineAssembly machinery complexBamA β-barrelChaperone-bound stateMembrane protein substratesRelated functional analysisLast β-strandFundamental biological processesOuter membrane proteinsDifferent conformational statesOMP biogenesisNutrition acquisitionGram-negative bacteriaProtein substratesMembrane proteinsΒ-barrelFunctional analysisBiological processesConformational states
2019
Conformational Dynamics, Intramolecular Domain Conformation Signaling, and Activation of Apo-FimD Revealed by Single-Molecule Fluorescence Resonance Energy Transfer Studies
Liu Y, Sun C, Han L, Yu Y, Zhou H, Shao Q, Lou J, Zhao Y, Huang Y. Conformational Dynamics, Intramolecular Domain Conformation Signaling, and Activation of Apo-FimD Revealed by Single-Molecule Fluorescence Resonance Energy Transfer Studies. Biochemistry 2019, 58: 1931-1941. PMID: 30888187, DOI: 10.1021/acs.biochem.9b00080.Peer-Reviewed Original ResearchConceptsC-terminal domainSingle-molecule fluorescence resonance energy transfer studiesN-terminal domainFluorescence resonance energy transfer studiesResonance energy transfer studiesConformational changesBacterial protein secretion systemsProtein secretion systemChaperone-subunit complexesDomain conformational changesSubstrate-bound stateOuter membrane proteinsPlug domainPilus biogenesisSubunit polymerizationSecretion pathwaySecretion systemMembrane proteinsDomain conformationConformational dynamicsMolecular eventsMolecular machinesEnergy transfer studiesConformational statesLinking studies
2016
Structure of the BAM complex and its implications for biogenesis of outer-membrane proteins
Han L, Zheng J, Wang Y, Yang X, Liu Y, Sun C, Cao B, Zhou H, Ni D, Lou J, Zhao Y, Huang Y. Structure of the BAM complex and its implications for biogenesis of outer-membrane proteins. Nature Structural & Molecular Biology 2016, 23: 192-196. PMID: 26900875, DOI: 10.1038/nsmb.3181.Peer-Reviewed Original Research