Conformational Dynamics, Intramolecular Domain Conformation Signaling, and Activation of Apo-FimD Revealed by Single-Molecule Fluorescence Resonance Energy Transfer Studies
Liu Y, Sun C, Han L, Yu Y, Zhou H, Shao Q, Lou J, Zhao Y, Huang Y. Conformational Dynamics, Intramolecular Domain Conformation Signaling, and Activation of Apo-FimD Revealed by Single-Molecule Fluorescence Resonance Energy Transfer Studies. Biochemistry 2019, 58: 1931-1941. PMID: 30888187, DOI: 10.1021/acs.biochem.9b00080.Peer-Reviewed Original ResearchConceptsC-terminal domainSingle-molecule fluorescence resonance energy transfer studiesN-terminal domainFluorescence resonance energy transfer studiesResonance energy transfer studiesConformational changesBacterial protein secretion systemsProtein secretion systemChaperone-subunit complexesDomain conformational changesSubstrate-bound stateOuter membrane proteinsPlug domainPilus biogenesisSubunit polymerizationSecretion pathwaySecretion systemMembrane proteinsDomain conformationConformational dynamicsMolecular eventsMolecular machinesEnergy transfer studiesConformational statesLinking studies