Modulation of c-fos and egr-1 expression in the isolated perfused kidney by agents that alter tubular work
Joannidis M, Cantley L, Spokes K, Stuart-Tilley A, Alper S, Epstein F. Modulation of c-fos and egr-1 expression in the isolated perfused kidney by agents that alter tubular work. Kidney International 1997, 52: 130-139. PMID: 9211355, DOI: 10.1038/ki.1997.312.Peer-Reviewed Original ResearchMeSH KeywordsAmino AcidsAnimalsBlotting, NorthernCell HypoxiaCells, CulturedDNA-Binding ProteinsDogsEarly Growth Response Protein 1Fluorescent Antibody Technique, IndirectGene Expression RegulationGlycineImmediate-Early ProteinsImmunohistochemistryIn Vitro TechniquesKidneyMaleOuabainProto-Oncogene Proteins c-fosRatsRats, Sprague-DawleyTime FactorsTranscription FactorsConceptsMedullary thick ascending limbThick ascending limbHypoxic injuryOuter medullaStandard perfusionC-fosMRNA levelsIEG expressionAscending limbEgr-1Immediate early gene c-fosAbsence of reperfusionEarly gene c-fosKrebs-Henseleit bufferGene c-fosImmediate early gene expressionInhibition of NaEgr-1 expressionHypoxic damageRenal cortexImmunohistochemical demonstrationRenal epithelial cellsTubular transportCultured renal epithelial cellsIEG mRNA levels