2023
Single-cell atlas of the human neonatal small intestine affected by necrotizing enterocolitis
Egozi A, Olaloye O, Werner L, Silva T, McCourt B, Pierce R, An X, Wang F, Chen K, Pober J, Shouval D, Itzkovitz S, Konnikova L. Single-cell atlas of the human neonatal small intestine affected by necrotizing enterocolitis. PLOS Biology 2023, 21: e3002124. PMID: 37205711, PMCID: PMC10234541, DOI: 10.1371/journal.pbio.3002124.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingSingle-cell atlasEpithelial cellsCell identityRNA sequencingBulk transcriptomicsCellular dysregulationAberrant interactionsNeonatal small intestinePotential targetCellular changesBiomarker discoveryGastrointestinal complicationsPremature infantsProinflammatory macrophagesProinflammatory genesClonal expansionT cellsEndothelial cellsImmune interactionsIntestinal tissueCellsSmall intestineComprehensive viewNEC
2022
Insights into the Role of Commensal-Specific T Cells in Intestinal Inflammation
Gehlhaar A, Inala A, Llivichuzhca-Loja D, Silva TN, Adegboye CY, O’Connell A, Konnikova L. Insights into the Role of Commensal-Specific T Cells in Intestinal Inflammation. Journal Of Inflammation Research 2022, 15: 1873-1887. PMID: 35342295, PMCID: PMC8943607, DOI: 10.2147/jir.s288288.Peer-Reviewed Original ResearchT cellsIntestinal inflammationIntestinal inflammatory responseDamaging autoimmune responsesTrillions of microorganismsImmunological balanceAutoimmune responseImmune homeostasisInflammatory responseImmune systemMucosal interfaceBarrier sitesHuman intestineInflammationIntestinePrevious evidenceCellsCD8CD4ThymusResponse
2018
CD117+ Exosomes and Mast Cells From Hereditary Alpha Tryptasemia Patients Reveal Unique Phosphorylation, Lipidomics, and Proteomics Profiles: Category Award (Small Intestine): Presidential Poster Award
Alli A, Konnikova L, Tuna K, Denslow N, Nouri M, Koh J, Li J, Tang Y, Shirley F, Ryan L, Lyons J, Milner J, Snapper S, Glover S. CD117+ Exosomes and Mast Cells From Hereditary Alpha Tryptasemia Patients Reveal Unique Phosphorylation, Lipidomics, and Proteomics Profiles: Category Award (Small Intestine): Presidential Poster Award. The American Journal Of Gastroenterology 2018, 113: s668. DOI: 10.14309/00000434-201810001-01173.Peer-Reviewed Original ResearchHigh-dimensional immune phenotyping and transcriptional analyses reveal robust recovery of viable human immune and epithelial cells from frozen gastrointestinal tissue
Konnikova L, Boschetti G, Rahman A, Mitsialis V, Lord J, Richmond C, Tomov VT, Gordon W, Jelinsky S, Canavan J, Liss A, Wall S, Field M, Zhou F, Goldsmith JD, Bewtra M, Breault DT, Merad M, Snapper SB. High-dimensional immune phenotyping and transcriptional analyses reveal robust recovery of viable human immune and epithelial cells from frozen gastrointestinal tissue. Mucosal Immunology 2018, 11: 1684-1693. PMID: 30111863, PMCID: PMC6512331, DOI: 10.1038/s41385-018-0047-y.Peer-Reviewed Original ResearchConceptsImmune cellsGastrointestinal tissuesMucosal immune compartmentPeripheral immune cellsMucosal immune cellsEpithelial cellsImmune compartmentImmune phenotypingTreatment responsivenessMucosal tissuesTranslational studiesDisease pathogenesisSimple storage techniquesPreservation of viabilityPathogenesisTissueBiomarker discoveryCellsDisease
2015
Individual intestinal symbionts induce a distinct population of RORγ+ regulatory T cells
Sefik E, Geva-Zatorsky N, Oh S, Konnikova L, Zemmour D, McGuire AM, Burzyn D, Ortiz-Lopez A, Lobera M, Yang J, Ghosh S, Earl A, Snapper SB, Jupp R, Kasper D, Mathis D, Benoist C. Individual intestinal symbionts induce a distinct population of RORγ+ regulatory T cells. Science 2015, 349: 993-997. PMID: 26272906, PMCID: PMC4700932, DOI: 10.1126/science.aaa9420.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacteriaBacteroidetesColitis, UlcerativeColonForkhead Transcription FactorsHomeostasisHumansImmunity, MucosalIntestinal MucosaMice, Inbred C57BLMicrobiotaNuclear Receptor Subfamily 1, Group F, Member 3SymbiosisTh17 CellsT-Lymphocyte SubsetsT-Lymphocytes, RegulatoryTranscription, GeneticTranscriptomeConceptsRegulatory T cellsImmuno-inflammatory responseT helper 17 (Th17) cell differentiationTranscription factor Foxp3Important effector moleculeRegulatory cellsHuman gut microbiotaFactor Foxp3T cellsRelated cell typesGut microbiotaMouse colonRORγSymbiotic membersIntestinal symbiontsFoxp3Tissue homeostasisEffector moleculesCell differentiationCell typesDistinct populationsCellsDifferent outcomesInflammation