Dnmt3a and Dnmt3b Have Overlapping and Distinct Functions in Hematopoietic Stem Cells
Challen G, Sun D, Mayle A, Jeong M, Luo M, Rodriguez B, Mallaney C, Celik H, Yang L, Xia Z, Cullen S, Berg J, Zheng Y, Darlington G, Li W, Goodell M. Dnmt3a and Dnmt3b Have Overlapping and Distinct Functions in Hematopoietic Stem Cells. Cell Stem Cell 2014, 15: 350-364. PMID: 25130491, PMCID: PMC4163922, DOI: 10.1016/j.stem.2014.06.018.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBeta CateninCell DifferentiationCell ProliferationCpG IslandsDNA (Cytosine-5-)-MethyltransferasesDNA MethylationDNA Methyltransferase 3AEpigenesis, GeneticGene Expression Regulation, DevelopmentalGene Regulatory NetworksHematopoietic Stem CellsIsoenzymesMice, Inbred C57BLMice, KnockoutNeoplasmsConceptsHematopoietic stem cellsDe novo methylation patternsAdult hematopoietic stem cellsHSC fate decisionsLoss of Dnmt3bStem cellsΒ-catenin signalingFate decisionsEpigenetic regulationMethylation patternsCpG islandsLifelong productionDistinct functionsHSC differentiationConditional inactivationDNMT3BHSC expansionDifferentiation blockSevere blockDistinct rolesImpaired differentiationDifferentiationMild phenotypeRegulationCells