2014
Influence of pH on Extracellular Matrix Preservation During Lung Decellularization
Tsuchiya T, Balestrini JL, Mendez J, Calle EA, Zhao L, Niklason LE. Influence of pH on Extracellular Matrix Preservation During Lung Decellularization. Tissue Engineering Part C Methods 2014, 20: 1028-1036. PMID: 24735501, PMCID: PMC4241865, DOI: 10.1089/ten.tec.2013.0492.Peer-Reviewed Original Research
2010
Tissue-Engineered Lungs for in Vivo Implantation
Petersen TH, Calle EA, Zhao L, Lee EJ, Gui L, Raredon MB, Gavrilov K, Yi T, Zhuang ZW, Breuer C, Herzog E, Niklason LE. Tissue-Engineered Lungs for in Vivo Implantation. Science 2010, 329: 538-541. PMID: 20576850, PMCID: PMC3640463, DOI: 10.1126/science.1189345.Peer-Reviewed Original ResearchConceptsLung tissueLung matrixAcellular lung matrixNative lung tissueTissue-engineered lungsLung transplantationPrimary therapyAdult lung tissueAdult ratsPulmonary epitheliumVascular endotheliumFunctional lungLung regenerationVascular compartmentLungSeeded endothelial cellsMechanical characteristicsEndothelial cellsVivo implantationRatsEpitheliumTissueCellular componentsExtracellular matrixGas exchangeUtility of Telomerase-pot1 Fusion Protein in Vascular Tissue Engineering
Petersen TH, Hitchcock T, Muto A, Calle EA, Zhao L, Gong Z, Gui L, Dardik A, Bowles DE, Counter CM, Niklason LE. Utility of Telomerase-pot1 Fusion Protein in Vascular Tissue Engineering. Cell Transplantation 2010, 19: 79-87. PMID: 19878625, PMCID: PMC2850951, DOI: 10.3727/096368909x478650.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAdultAnimalsBioreactorsBlood VesselsCell Culture TechniquesCells, CulturedCellular SenescenceCollagenGenetic VectorsGraft SurvivalHumansMaleMuscle, Smooth, VascularRatsRats, NudeRecombinant Fusion ProteinsShelterin ComplexTelomeraseTelomere-Binding ProteinsTissue EngineeringTransfectionConceptsTransient deliveryVascular tissue engineeringRegenerative medicineTissue engineeringRegenerative medicine applicationsTissue-engineered constructsLentiviral vectorsMedicine applicationsImportant stumbling blockTelomeric repeat amplification protocolElderly human donorsBetter performanceAmplification protocolEngineeringDeliveryTransient reconstitutionDifferentiated cellsAdenoviral deliveryRepeat amplification protocolFusion proteinTransgeneHuman smooth muscle cellsStumbling blockGreater collagen contentProtocol
2007
Alloimmune-Mediated Vascular Remodeling of Human Coronary Artery Grafts in Immunodeficient Mouse Recipients Is Independent of Preexisting Atherosclerosis
Wang Y, Ahmad U, Yi T, Zhao L, Lorber MI, Pober JS, Tellides G. Alloimmune-Mediated Vascular Remodeling of Human Coronary Artery Grafts in Immunodeficient Mouse Recipients Is Independent of Preexisting Atherosclerosis. Transplantation 2007, 83: 1501-1505. PMID: 17565324, DOI: 10.1097/01.tp.0000264560.51845.67.Peer-Reviewed Original ResearchConceptsImmunodeficient mouse recipientsHuman coronary artery graftsCoronary artery graftsVascular remodelingArtery graftCoronary atherosclerosisMouse recipientsAllogeneic human peripheral blood mononuclear cellsHuman peripheral blood mononuclear cellsPeripheral blood mononuclear cellsBlood mononuclear cellsArterial injuryGraft arteriosclerosisIntimal injuryLuminal lossImmunosuppressive drugsLumen lossMononuclear cellsIntimal thickeningClinical studiesIntimal expansionAtherosclerotic lesionsOrgan donorsChimeric modelAtherosclerosis