2016
Comparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine
Balestrini JL, Gard AL, Gerhold KA, Wilcox EC, Liu A, Schwan J, Le AV, Baevova P, Dimitrievska S, Zhao L, Sundaram S, Sun H, Rittié L, Dyal R, Broekelmann TJ, Mecham RP, Schwartz MA, Niklason LE, White ES. Comparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine. Biomaterials 2016, 102: 220-230. PMID: 27344365, PMCID: PMC4939101, DOI: 10.1016/j.biomaterials.2016.06.025.Peer-Reviewed Original ResearchConceptsHuman endothelial cellsCell-matrix interactionsLung regenerationEndothelial cellsKey matrix proteinsComparative biologyCell adhesion moleculeMatrix proteinsLung extracellular matrixCell healthExtracellular matrixResidual DNASpecies mismatchRat lung scaffoldsRegenerative medicineAdhesion moleculesLung scaffoldsPrimate tissuesCellsVascular cell adhesion moleculeLung engineeringLung matrixLess expressionPulmonary cellsProfound effectBiaxial Stretch Improves Elastic Fiber Maturation, Collagen Arrangement, and Mechanical Properties in Engineered Arteries
Huang AH, Balestrini JL, Udelsman BV, Zhou KC, Zhao L, Ferruzzi J, Starcher BC, Levene MJ, Humphrey JD, Niklason LE. Biaxial Stretch Improves Elastic Fiber Maturation, Collagen Arrangement, and Mechanical Properties in Engineered Arteries. Tissue Engineering Part C Methods 2016, 22: 524-533. PMID: 27108525, PMCID: PMC4921901, DOI: 10.1089/ten.tec.2015.0309.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArteriesBioreactorsCollagenElastic TissueExtracellular MatrixHumansRegenerationStress, MechanicalTissue EngineeringConceptsTissue-engineered blood vesselsBiaxial loadingMechanical propertiesMechanical strengthFiber orientationMultiaxial loadingLoading conditionsMechanical integrityBiaxial stretchingCollagen undulationArtificial skinNovel bioreactorMechanical failureMatrix orientationBiaxial stretchLoadingAxial stretchCollagen fiber orientationSuture strengthNative arteriesTissue equivalentsStrengthPropertiesCircumferential stretchMatrix contentSterilization of Lung Matrices by Supercritical Carbon Dioxide
Balestrini JL, Liu A, Gard AL, Huie J, Blatt KM, Schwan J, Zhao L, Broekelmann TJ, Mecham RP, Wilcox EC, Niklason LE. Sterilization of Lung Matrices by Supercritical Carbon Dioxide. Tissue Engineering Part C Methods 2016, 22: 260-269. PMID: 26697757, PMCID: PMC4782026, DOI: 10.1089/ten.tec.2015.0449.Peer-Reviewed Original ResearchMeSH KeywordsA549 CellsAnimalsCarbon DioxideExtracellular MatrixHumansLungMechanical PhenomenaPeracetic AcidRats, Sprague-DawleySterilizationTissue ScaffoldsConceptsLung matrixEnd-stage pulmonary failureExtracellular matrix depletionMajority of lungSupercritical carbon dioxidePulmonary failureLung tissueAcellular lungsCell engraftmentLungCurrent study resultsLung engineeringMatrix depletionTissueMechanical integrityCarbon dioxideScCO2 treatmentMechanical featuresDecellularized scaffoldTissue architectureSterile bufferSterility assurance levelDecellularized tissuesRegenerative medicine purposes
2014
Tissue‐Engineered Vascular Grafts Created From Human Induced Pluripotent Stem Cells
Sundaram S, One J, Siewert J, Teodosescu S, Zhao L, Dimitrievska S, Qian H, Huang AH, Niklason L. Tissue‐Engineered Vascular Grafts Created From Human Induced Pluripotent Stem Cells. Stem Cells Translational Medicine 2014, 3: 1535-1543. PMID: 25378654, PMCID: PMC4250208, DOI: 10.5966/sctm.2014-0065.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, DifferentiationBlood Vessel ProsthesisCell LineExtracellular MatrixHumansInduced Pluripotent Stem CellsTissue EngineeringConceptsPluripotent stem cellsMesenchymal lineagesSmooth muscle cell differentiationMuscle cell differentiationStem cellsInduced pluripotent stem cellsNeural crest intermediateHuman induced pluripotent stem cellsMesenchymal progenitor cellsStem cell clonesCollagen-rich matrixCell differentiationVascular developmentHiPSC linesProgenitor cellsSmooth muscle cellsCell apoptosisHiPS cellsLineagesMesenchymal markersGeneration of graftMuscle cellsClonesTissue-engineered vascular graftsCellsInfluence of pH on Extracellular Matrix Preservation During Lung Decellularization
Tsuchiya T, Balestrini JL, Mendez J, Calle EA, Zhao L, Niklason LE. Influence of pH on Extracellular Matrix Preservation During Lung Decellularization. Tissue Engineering Part C Methods 2014, 20: 1028-1036. PMID: 24735501, PMCID: PMC4241865, DOI: 10.1089/ten.tec.2013.0492.Peer-Reviewed Original ResearchThe Use of Optical Clearing and Multiphoton Microscopy for Investigation of Three-Dimensional Tissue-Engineered Constructs
Calle EA, Vesuna S, Dimitrievska S, Zhou K, Huang A, Zhao L, Niklason LE, Levene MJ. The Use of Optical Clearing and Multiphoton Microscopy for Investigation of Three-Dimensional Tissue-Engineered Constructs. Tissue Engineering Part C Methods 2014, 20: 570-577. PMID: 24251630, PMCID: PMC4074743, DOI: 10.1089/ten.tec.2013.0538.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Line, TumorExtracellular MatrixHumansLungMicroscopy, Fluorescence, MultiphotonRatsTissue EngineeringTissue ScaffoldsConceptsTissue-engineered blood vesselsThree-dimensional tissue engineeringThree-dimensional tissuesTissue engineeringEngineered ConstructsMicron scaleExtracellular matrix scaffoldsIntact volumesNondestructive imagingMatrix scaffoldsSimple separationVirtual volumeNew methodMicroscopyVessel integrityIsotropic resolutionDigital volumeIndividual collagen fibersSingle planeNondestructive measuresEngineeringStackMethodRegistration algorithmStack of images
2010
Tissue-Engineered Lungs for in Vivo Implantation
Petersen TH, Calle EA, Zhao L, Lee EJ, Gui L, Raredon MB, Gavrilov K, Yi T, Zhuang ZW, Breuer C, Herzog E, Niklason LE. Tissue-Engineered Lungs for in Vivo Implantation. Science 2010, 329: 538-541. PMID: 20576850, PMCID: PMC3640463, DOI: 10.1126/science.1189345.Peer-Reviewed Original ResearchConceptsLung tissueLung matrixAcellular lung matrixNative lung tissueTissue-engineered lungsLung transplantationPrimary therapyAdult lung tissueAdult ratsPulmonary epitheliumVascular endotheliumFunctional lungLung regenerationVascular compartmentLungSeeded endothelial cellsMechanical characteristicsEndothelial cellsVivo implantationRatsEpitheliumTissueCellular componentsExtracellular matrixGas exchange