2020
Tissue-Engineered Vascular Grafts with Advanced Mechanical Strength from Human iPSCs
Luo J, Qin L, Zhao L, Gui L, Ellis MW, Huang Y, Kural MH, Clark JA, Ono S, Wang J, Yuan Y, Zhang SM, Cong X, Li G, Riaz M, Lopez C, Hotta A, Campbell S, Tellides G, Dardik A, Niklason LE, Qyang Y. Tissue-Engineered Vascular Grafts with Advanced Mechanical Strength from Human iPSCs. Cell Stem Cell 2020, 26: 251-261.e8. PMID: 31956039, PMCID: PMC7021512, DOI: 10.1016/j.stem.2019.12.012.Peer-Reviewed Original ResearchMeSH KeywordsBlood Vessel ProsthesisHumansInduced Pluripotent Stem CellsMyocytes, Smooth MuscleTissue Engineering
2016
Engineered Tissue–Stent Biocomposites as Tracheal Replacements
Zhao L, Sundaram S, Le AV, Huang AH, Zhang J, Hatachi G, Beloiartsev A, Caty MG, Yi T, Leiby K, Gard A, Kural MH, Gui L, Rocco KA, Sivarapatna A, Calle E, Greaney A, Urbani L, Maghsoudlou P, Burns A, DeCoppi P, Niklason LE. Engineered Tissue–Stent Biocomposites as Tracheal Replacements. Tissue Engineering Part A 2016, 22: 1086-1097. PMID: 27520928, PMCID: PMC5312617, DOI: 10.1089/ten.tea.2016.0132.Peer-Reviewed Original ResearchComparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine
Balestrini JL, Gard AL, Gerhold KA, Wilcox EC, Liu A, Schwan J, Le AV, Baevova P, Dimitrievska S, Zhao L, Sundaram S, Sun H, Rittié L, Dyal R, Broekelmann TJ, Mecham RP, Schwartz MA, Niklason LE, White ES. Comparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine. Biomaterials 2016, 102: 220-230. PMID: 27344365, PMCID: PMC4939101, DOI: 10.1016/j.biomaterials.2016.06.025.Peer-Reviewed Original ResearchConceptsHuman endothelial cellsCell-matrix interactionsLung regenerationEndothelial cellsKey matrix proteinsComparative biologyCell adhesion moleculeMatrix proteinsLung extracellular matrixCell healthExtracellular matrixResidual DNASpecies mismatchRat lung scaffoldsRegenerative medicineAdhesion moleculesLung scaffoldsPrimate tissuesCellsVascular cell adhesion moleculeLung engineeringLung matrixLess expressionPulmonary cellsProfound effectImplantable tissue-engineered blood vessels from human induced pluripotent stem cells
Gui L, Dash BC, Luo J, Qin L, Zhao L, Yamamoto K, Hashimoto T, Wu H, Dardik A, Tellides G, Niklason LE, Qyang Y. Implantable tissue-engineered blood vessels from human induced pluripotent stem cells. Biomaterials 2016, 102: 120-129. PMID: 27336184, PMCID: PMC4939127, DOI: 10.1016/j.biomaterials.2016.06.010.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsVascular diseaseBlood vesselsAlpha-smooth muscle actinSmooth muscle myosin heavy chainActive vascular remodelingSmooth muscle cellsMuscle myosin heavy chainTissue-engineered blood vesselsStem cellsAbundant collagenous matrixPluripotent stem cellsInterposition graftAllogeneic graftsVascular remodelingΑ-SMANude ratsMuscle actinMyosin heavy chainClinical useMuscle cellsFunctional vascular smooth muscle cellsPatientsFunctional tissue-engineered blood vesselGraftBiaxial Stretch Improves Elastic Fiber Maturation, Collagen Arrangement, and Mechanical Properties in Engineered Arteries
Huang AH, Balestrini JL, Udelsman BV, Zhou KC, Zhao L, Ferruzzi J, Starcher BC, Levene MJ, Humphrey JD, Niklason LE. Biaxial Stretch Improves Elastic Fiber Maturation, Collagen Arrangement, and Mechanical Properties in Engineered Arteries. Tissue Engineering Part C Methods 2016, 22: 524-533. PMID: 27108525, PMCID: PMC4921901, DOI: 10.1089/ten.tec.2015.0309.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArteriesBioreactorsCollagenElastic TissueExtracellular MatrixHumansRegenerationStress, MechanicalTissue EngineeringConceptsTissue-engineered blood vesselsBiaxial loadingMechanical propertiesMechanical strengthFiber orientationMultiaxial loadingLoading conditionsMechanical integrityBiaxial stretchingCollagen undulationArtificial skinNovel bioreactorMechanical failureMatrix orientationBiaxial stretchLoadingAxial stretchCollagen fiber orientationSuture strengthNative arteriesTissue equivalentsStrengthPropertiesCircumferential stretchMatrix content
2014
Tissue‐Engineered Vascular Grafts Created From Human Induced Pluripotent Stem Cells
Sundaram S, One J, Siewert J, Teodosescu S, Zhao L, Dimitrievska S, Qian H, Huang AH, Niklason L. Tissue‐Engineered Vascular Grafts Created From Human Induced Pluripotent Stem Cells. Stem Cells Translational Medicine 2014, 3: 1535-1543. PMID: 25378654, PMCID: PMC4250208, DOI: 10.5966/sctm.2014-0065.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, DifferentiationBlood Vessel ProsthesisCell LineExtracellular MatrixHumansInduced Pluripotent Stem CellsTissue EngineeringConceptsPluripotent stem cellsMesenchymal lineagesSmooth muscle cell differentiationMuscle cell differentiationStem cellsInduced pluripotent stem cellsNeural crest intermediateHuman induced pluripotent stem cellsMesenchymal progenitor cellsStem cell clonesCollagen-rich matrixCell differentiationVascular developmentHiPSC linesProgenitor cellsSmooth muscle cellsCell apoptosisHiPS cellsLineagesMesenchymal markersGeneration of graftMuscle cellsClonesTissue-engineered vascular graftsCellsInfluence of pH on Extracellular Matrix Preservation During Lung Decellularization
Tsuchiya T, Balestrini JL, Mendez J, Calle EA, Zhao L, Niklason LE. Influence of pH on Extracellular Matrix Preservation During Lung Decellularization. Tissue Engineering Part C Methods 2014, 20: 1028-1036. PMID: 24735501, PMCID: PMC4241865, DOI: 10.1089/ten.tec.2013.0492.Peer-Reviewed Original ResearchThe Use of Optical Clearing and Multiphoton Microscopy for Investigation of Three-Dimensional Tissue-Engineered Constructs
Calle EA, Vesuna S, Dimitrievska S, Zhou K, Huang A, Zhao L, Niklason LE, Levene MJ. The Use of Optical Clearing and Multiphoton Microscopy for Investigation of Three-Dimensional Tissue-Engineered Constructs. Tissue Engineering Part C Methods 2014, 20: 570-577. PMID: 24251630, PMCID: PMC4074743, DOI: 10.1089/ten.tec.2013.0538.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Line, TumorExtracellular MatrixHumansLungMicroscopy, Fluorescence, MultiphotonRatsTissue EngineeringTissue ScaffoldsConceptsTissue-engineered blood vesselsThree-dimensional tissue engineeringThree-dimensional tissuesTissue engineeringEngineered ConstructsMicron scaleExtracellular matrix scaffoldsIntact volumesNondestructive imagingMatrix scaffoldsSimple separationVirtual volumeNew methodMicroscopyVessel integrityIsotropic resolutionDigital volumeIndividual collagen fibersSingle planeNondestructive measuresEngineeringStackMethodRegistration algorithmStack of images
2013
Fibroblast engraftment in the decellularized mouse lung occurs via a β1-integrin-dependent, FAK-dependent pathway that is mediated by ERK and opposed by AKT
Sun H, Calle E, Chen X, Mathur A, Zhu Y, Mendez J, Zhao L, Niklason L, Peng X, Peng H, Herzog EL. Fibroblast engraftment in the decellularized mouse lung occurs via a β1-integrin-dependent, FAK-dependent pathway that is mediated by ERK and opposed by AKT. American Journal Of Physiology - Lung Cellular And Molecular Physiology 2013, 306: l463-l475. PMID: 24337923, PMCID: PMC3949086, DOI: 10.1152/ajplung.00100.2013.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, NeutralizingBioartificial OrgansCell AdhesionCell LineCell ProliferationCell SurvivalExtracellular Signal-Regulated MAP KinasesFibroblastsFocal Adhesion Kinase 1Integrin beta1LungMicePhosphorylationProto-Oncogene Proteins c-aktRatsRho-Associated KinasesTissue EngineeringTissue ScaffoldsConceptsExtracellular signal-regulated kinase (ERK) inhibitorSignal-regulated kinase inhibitorKinase inhibitorsERK-dependent mannerFAK-dependent pathwayFocal adhesion kinase (FAK) inhibitorFibroblast cell lineMouse fibroblast cell lineTissue-engineered lungsMinimal cell deathCell survivalCell deathMouse lungAkt inhibitorMouse fibroblastsProteinaceous componentsMammalian lungCell proliferationCell linesNumber of mechanismsAktTime-dependent increaseLung scaffoldsCell numberCell density
2011
Development of Novel Biodegradable Polymer Scaffolds for Vascular Tissue Engineering
Gui L, Zhao L, Spencer RW, Burghouwt A, Taylor MS, Shalaby SW, Niklason LE. Development of Novel Biodegradable Polymer Scaffolds for Vascular Tissue Engineering. Tissue Engineering Part A 2011, 17: 1191-1200. PMID: 21143045, PMCID: PMC3079248, DOI: 10.1089/ten.tea.2010.0508.Peer-Reviewed Original ResearchMeSH KeywordsAbsorbable ImplantsAnimalsBlood Vessel ProsthesisMaterials TestingPolymersSwineTissue EngineeringTissue ScaffoldsConceptsTissue engineering approachesTissue-engineered blood vesselsBiodegradable polymer scaffoldsVascular tissue engineeringPolyglycolic acidDegradation profileTissue mechanicsEngineering approachVessel mechanicsPolymers IIIPolymer scaffoldsBiodegradable scaffoldsTissue engineeringPolymeric materialsDegradation characteristicsMatrix-rich tissuesSynthetic polymersPolymer IPolymer IIPolymer fragmentsAqueous conditionsPolymersPotential applicationsSimilar degradation profilesMechanics
2010
Tissue-Engineered Lungs for in Vivo Implantation
Petersen TH, Calle EA, Zhao L, Lee EJ, Gui L, Raredon MB, Gavrilov K, Yi T, Zhuang ZW, Breuer C, Herzog E, Niklason LE. Tissue-Engineered Lungs for in Vivo Implantation. Science 2010, 329: 538-541. PMID: 20576850, PMCID: PMC3640463, DOI: 10.1126/science.1189345.Peer-Reviewed Original ResearchConceptsLung tissueLung matrixAcellular lung matrixNative lung tissueTissue-engineered lungsLung transplantationPrimary therapyAdult lung tissueAdult ratsPulmonary epitheliumVascular endotheliumFunctional lungLung regenerationVascular compartmentLungSeeded endothelial cellsMechanical characteristicsEndothelial cellsVivo implantationRatsEpitheliumTissueCellular componentsExtracellular matrixGas exchangeUtility of Telomerase-pot1 Fusion Protein in Vascular Tissue Engineering
Petersen TH, Hitchcock T, Muto A, Calle EA, Zhao L, Gong Z, Gui L, Dardik A, Bowles DE, Counter CM, Niklason LE. Utility of Telomerase-pot1 Fusion Protein in Vascular Tissue Engineering. Cell Transplantation 2010, 19: 79-87. PMID: 19878625, PMCID: PMC2850951, DOI: 10.3727/096368909x478650.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAdultAnimalsBioreactorsBlood VesselsCell Culture TechniquesCells, CulturedCellular SenescenceCollagenGenetic VectorsGraft SurvivalHumansMaleMuscle, Smooth, VascularRatsRats, NudeRecombinant Fusion ProteinsShelterin ComplexTelomeraseTelomere-Binding ProteinsTissue EngineeringTransfectionConceptsTransient deliveryVascular tissue engineeringRegenerative medicineTissue engineeringRegenerative medicine applicationsTissue-engineered constructsLentiviral vectorsMedicine applicationsImportant stumbling blockTelomeric repeat amplification protocolElderly human donorsBetter performanceAmplification protocolEngineeringDeliveryTransient reconstitutionDifferentiated cellsAdenoviral deliveryRepeat amplification protocolFusion proteinTransgeneHuman smooth muscle cellsStumbling blockGreater collagen contentProtocol