2014
The Use of Optical Clearing and Multiphoton Microscopy for Investigation of Three-Dimensional Tissue-Engineered Constructs
Calle EA, Vesuna S, Dimitrievska S, Zhou K, Huang A, Zhao L, Niklason LE, Levene MJ. The Use of Optical Clearing and Multiphoton Microscopy for Investigation of Three-Dimensional Tissue-Engineered Constructs. Tissue Engineering Part C Methods 2014, 20: 570-577. PMID: 24251630, PMCID: PMC4074743, DOI: 10.1089/ten.tec.2013.0538.Peer-Reviewed Original ResearchConceptsTissue-engineered blood vesselsThree-dimensional tissue engineeringThree-dimensional tissuesTissue engineeringEngineered ConstructsMicron scaleExtracellular matrix scaffoldsIntact volumesNondestructive imagingMatrix scaffoldsSimple separationVirtual volumeNew methodMicroscopyVessel integrityIsotropic resolutionDigital volumeIndividual collagen fibersSingle planeNondestructive measuresEngineeringStackMethodRegistration algorithmStack of images
2011
Development of Novel Biodegradable Polymer Scaffolds for Vascular Tissue Engineering
Gui L, Zhao L, Spencer RW, Burghouwt A, Taylor MS, Shalaby SW, Niklason LE. Development of Novel Biodegradable Polymer Scaffolds for Vascular Tissue Engineering. Tissue Engineering Part A 2011, 17: 1191-1200. PMID: 21143045, PMCID: PMC3079248, DOI: 10.1089/ten.tea.2010.0508.Peer-Reviewed Original ResearchConceptsTissue engineering approachesTissue-engineered blood vesselsBiodegradable polymer scaffoldsVascular tissue engineeringPolyglycolic acidDegradation profileTissue mechanicsEngineering approachVessel mechanicsPolymers IIIPolymer scaffoldsBiodegradable scaffoldsTissue engineeringPolymeric materialsDegradation characteristicsMatrix-rich tissuesSynthetic polymersPolymer IPolymer IIPolymer fragmentsAqueous conditionsPolymersPotential applicationsSimilar degradation profilesMechanics
2010
Utility of Telomerase-pot1 Fusion Protein in Vascular Tissue Engineering
Petersen TH, Hitchcock T, Muto A, Calle EA, Zhao L, Gong Z, Gui L, Dardik A, Bowles DE, Counter CM, Niklason LE. Utility of Telomerase-pot1 Fusion Protein in Vascular Tissue Engineering. Cell Transplantation 2010, 19: 79-87. PMID: 19878625, PMCID: PMC2850951, DOI: 10.3727/096368909x478650.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAdultAnimalsBioreactorsBlood VesselsCell Culture TechniquesCells, CulturedCellular SenescenceCollagenGenetic VectorsGraft SurvivalHumansMaleMuscle, Smooth, VascularRatsRats, NudeRecombinant Fusion ProteinsShelterin ComplexTelomeraseTelomere-Binding ProteinsTissue EngineeringTransfectionConceptsTransient deliveryVascular tissue engineeringRegenerative medicineTissue engineeringRegenerative medicine applicationsTissue-engineered constructsLentiviral vectorsMedicine applicationsImportant stumbling blockTelomeric repeat amplification protocolElderly human donorsBetter performanceAmplification protocolEngineeringDeliveryTransient reconstitutionDifferentiated cellsAdenoviral deliveryRepeat amplification protocolFusion proteinTransgeneHuman smooth muscle cellsStumbling blockGreater collagen contentProtocol