Biaxial Stretch Improves Elastic Fiber Maturation, Collagen Arrangement, and Mechanical Properties in Engineered Arteries
Huang AH, Balestrini JL, Udelsman BV, Zhou KC, Zhao L, Ferruzzi J, Starcher BC, Levene MJ, Humphrey JD, Niklason LE. Biaxial Stretch Improves Elastic Fiber Maturation, Collagen Arrangement, and Mechanical Properties in Engineered Arteries. Tissue Engineering Part C Methods 2016, 22: 524-533. PMID: 27108525, PMCID: PMC4921901, DOI: 10.1089/ten.tec.2015.0309.Peer-Reviewed Original ResearchConceptsTissue-engineered blood vesselsBiaxial loadingMechanical propertiesMechanical strengthFiber orientationMultiaxial loadingLoading conditionsMechanical integrityBiaxial stretchingCollagen undulationArtificial skinNovel bioreactorMechanical failureMatrix orientationBiaxial stretchLoadingAxial stretchCollagen fiber orientationSuture strengthNative arteriesTissue equivalentsStrengthPropertiesCircumferential stretchMatrix contentSterilization of Lung Matrices by Supercritical Carbon Dioxide
Balestrini JL, Liu A, Gard AL, Huie J, Blatt KM, Schwan J, Zhao L, Broekelmann TJ, Mecham RP, Wilcox EC, Niklason LE. Sterilization of Lung Matrices by Supercritical Carbon Dioxide. Tissue Engineering Part C Methods 2016, 22: 260-269. PMID: 26697757, PMCID: PMC4782026, DOI: 10.1089/ten.tec.2015.0449.Peer-Reviewed Original ResearchConceptsLung matrixEnd-stage pulmonary failureExtracellular matrix depletionMajority of lungSupercritical carbon dioxidePulmonary failureLung tissueAcellular lungsCell engraftmentLungCurrent study resultsLung engineeringMatrix depletionTissueMechanical integrityCarbon dioxideScCO2 treatmentMechanical featuresDecellularized scaffoldTissue architectureSterile bufferSterility assurance levelDecellularized tissuesRegenerative medicine purposes