The Use of Optical Clearing and Multiphoton Microscopy for Investigation of Three-Dimensional Tissue-Engineered Constructs
Calle EA, Vesuna S, Dimitrievska S, Zhou K, Huang A, Zhao L, Niklason LE, Levene MJ. The Use of Optical Clearing and Multiphoton Microscopy for Investigation of Three-Dimensional Tissue-Engineered Constructs. Tissue Engineering Part C Methods 2014, 20: 570-577. PMID: 24251630, PMCID: PMC4074743, DOI: 10.1089/ten.tec.2013.0538.Peer-Reviewed Original ResearchConceptsTissue-engineered blood vesselsThree-dimensional tissue engineeringThree-dimensional tissuesTissue engineeringEngineered ConstructsMicron scaleExtracellular matrix scaffoldsIntact volumesNondestructive imagingMatrix scaffoldsSimple separationVirtual volumeNew methodMicroscopyVessel integrityIsotropic resolutionDigital volumeIndividual collagen fibersSingle planeNondestructive measuresEngineeringStackMethodRegistration algorithmStack of images