2021
IL-10 Deficiency Accelerates Type 1 Diabetes Development via Modulation of Innate and Adaptive Immune Cells and Gut Microbiota in BDC2.5 NOD Mice
Huang J, Tan Q, Tai N, Pearson JA, Li Y, Chao C, Zhang L, Peng J, Xing Y, Zhang L, Hu Y, Zhou Z, Wong FS, Wen L. IL-10 Deficiency Accelerates Type 1 Diabetes Development via Modulation of Innate and Adaptive Immune Cells and Gut Microbiota in BDC2.5 NOD Mice. Frontiers In Immunology 2021, 12: 702955. PMID: 34394099, PMCID: PMC8362616, DOI: 10.3389/fimmu.2021.702955.Peer-Reviewed Original ResearchConceptsNOD miceProportion of neutrophilsT cellsGut microbiotaDiabetes developmentT cell-mediated destructionT cell receptor transgenicType 1 diabetes developmentAccelerated diabetes developmentInhibition of diabetesModulation of InnatePathogenicity of CD4Cell-mediated destructionAdaptive immune cellsObese diabetic miceT regulatory (Treg) cellsDevelopment of diabetesPrevention of diabetesActivation of CD4Modulation of neutrophilsType 1 diabetesGut microbiota compositionInsulin-producing β-cellsSevere insulitisSpontaneous diabetesInnate immunity in latent autoimmune diabetes in adults
Huang J, Pearson JA, Wong FS, Wen L, Zhou Z. Innate immunity in latent autoimmune diabetes in adults. Diabetes/Metabolism Research And Reviews 2021, 38: e3480. PMID: 34156143, PMCID: PMC8813511, DOI: 10.1002/dmrr.3480.Peer-Reviewed Original ResearchConceptsType 1 diabetesDendritic cellsImmune cellsT cellsInnate immunityPathogenesis of LADALatent autoimmune diabetesAdaptive immune cellsPancreas of patientsType 2 diabetesImmune-associated genesIslet β-cellsAutoimmune diabetesClinical featuresImmunological reasonsAutoimmune diseasesRat modelB cellsDiabetesΒ-cellsImmunityPotential rolePathogenesisLADADiseaseInflammasomes and Type 1 Diabetes
Pearson JA, Wong FS, Wen L. Inflammasomes and Type 1 Diabetes. Frontiers In Immunology 2021, 12: 686956. PMID: 34177937, PMCID: PMC8219953, DOI: 10.3389/fimmu.2021.686956.Peer-Reviewed Original ResearchConceptsType 1 diabetesMultiprotein complexesEnhanced toleranceMicrobial ligandsIslet autoantibody developmentImmune responseGenetic associationMicrobial stimulationAvailable inhibitorsImportant modulatorType 1 diabetes susceptibilityPathwayDiabetes susceptibilityAutoimmune processMicrobiota compositionAutoantibody developmentMicrobiotaAnimal modelsInflammasomeActivationGenetic riskType 1DiabetesHumansRoleToll-like receptor 7 deficiency suppresses type 1 diabetes development by modulating B-cell differentiation and function
Huang J, Peng J, Pearson JA, Efthimiou G, Hu Y, Tai N, Xing Y, Zhang L, Gu J, Jiang J, Zhao H, Zhou Z, Wong FS, Wen L. Toll-like receptor 7 deficiency suppresses type 1 diabetes development by modulating B-cell differentiation and function. Cellular & Molecular Immunology 2021, 18: 328-338. PMID: 33432061, PMCID: PMC8027372, DOI: 10.1038/s41423-020-00590-8.Peer-Reviewed Original ResearchConceptsType 1 diabetes developmentToll-like receptorsType 1 diabetesDiabetes developmentB cellsTLR7 deficiencyNOD miceB cell differentiationT cellsClassical MHC class I moleculesHuman type 1 diabetesImmunodeficient NOD miceNOD B cellsDiabetogenic T cellsAntigen-presenting functionNonobese diabetic (NOD) miceT cell responsesB cell functionMHC class I moleculesPattern recognition receptorsT cell activationPathogen molecular patternsClass I moleculesDiabetogenic CD4Cytotoxic CD8
2014
Epicutaneous Immunization with TNP-Ig and Zymosan Induces TCRαβ+ CD4+ Contrasuppressor Cells That Reverse Skin-Induced Suppression via IL-17A
Majewska-Szczepanik M, Strzepa A, Marcińska K, Wen L, Szczepanik M. Epicutaneous Immunization with TNP-Ig and Zymosan Induces TCRαβ+ CD4+ Contrasuppressor Cells That Reverse Skin-Induced Suppression via IL-17A. International Archives Of Allergy And Immunology 2014, 164: 122-136. PMID: 24993442, PMCID: PMC4141016, DOI: 10.1159/000363446.Peer-Reviewed Original ResearchMeSH KeywordsAdministration, CutaneousAnimalsAntigensCD4-Positive T-LymphocytesDermatitis, ContactHaptensImmunity, InnateImmunizationImmunoglobulinsImmunosuppression TherapyInterleukin-17Lymph NodesMiceMice, Inbred C57BLMice, Inbred CBAMyeloid Differentiation Factor 88Receptors, Antigen, T-Cell, alpha-betaSkinToll-Like Receptor 2Transforming Growth Factor betaTrinitrobenzenesVaccinationZymosanConceptsSkin-induced suppressionSuppression of CHSContact hypersensitivityEC immunizationEpicutaneous immunizationTNP-IgAdoptive cell transfer experimentsProtein antigensT contrasuppressor cellsT suppressor cellsLymph node cellsCell transfer experimentsCHS responseContrasuppressor cellsIL-17ASuppressor cellsCytokine productionNode cellsImmunogenic antigensPresence of zymosanAntigen E.ImmunizationInnate immunityCD4Gauze patches
2013
Immunotherapy for T1DM—targeting innate immunity
Wong F, Wen L. Immunotherapy for T1DM—targeting innate immunity. Nature Reviews Endocrinology 2013, 9: 384-385. PMID: 23732280, PMCID: PMC4048745, DOI: 10.1038/nrendo.2013.103.Peer-Reviewed Original Research
2009
Innate and adaptive immune responses are highly interconnected at many levels.
Wong F, Wen L. Innate and adaptive immune responses are highly interconnected at many levels. 2009, 9: 1-3. PMID: 19199936, DOI: 10.2174/156652409787314462.Peer-Reviewed Original Research
2008
Toll‐Like Receptors and Diabetes
Wong F, Wen L. Toll‐Like Receptors and Diabetes. Annals Of The New York Academy Of Sciences 2008, 1150: 123-132. PMID: 19120280, DOI: 10.1196/annals.1447.063.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutoimmunityDiabetes MellitusHumansImmunity, InnateInfectionsModels, BiologicalT-Lymphocytes, RegulatoryToll-Like ReceptorsConceptsToll-like receptorsAntigen-presenting cellsType 1 interferonAdaptive immune systemRegulatory cellsAutoimmune responseInflammatory cytokinesMore specific responsesIFN-alphaImmune responseEndogenous ligandImmune systemMolecular patternsInfectionMicrobial infectionsReceptorsInterferonEndogenous stimuliDirect effectCellular stressSpecific responsesCellsResponseAutoimmunityDiabetesInnate immunity and intestinal microbiota in the development of Type 1 diabetes
Wen L, Ley RE, Volchkov PY, Stranges PB, Avanesyan L, Stonebraker AC, Hu C, Wong FS, Szot GL, Bluestone JA, Gordon JI, Chervonsky AV. Innate immunity and intestinal microbiota in the development of Type 1 diabetes. Nature 2008, 455: 1109-1113. PMID: 18806780, PMCID: PMC2574766, DOI: 10.1038/nature07336.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacteriaCD8-Positive T-LymphocytesDiabetes Mellitus, Type 1FemaleImmunity, InnateInterferon-gammaIntestinesIslets of LangerhansMaleMiceMice, Inbred NODMice, KnockoutMice, SCIDMolecular Sequence DataMyeloid Differentiation Factor 88PhylogenySpecific Pathogen-Free OrganismsTime FactorsConceptsType 1 diabetesNOD miceInnate immunityRapid innate immune responseDevelopment of diabetesNormal human gutInnate immune responseAdaptor protein MyD88Autoimmune diabetesTherapeutic optionsImmune responseNegative miceIntestinal microbiotaProtein MyD88DiabetesMiceGut microbesImmunityHuman gutMicrobial productsMyD88Influence predispositionIncidence