1994
Overexpression of parathyroid hormone-related protein in the skin of transgenic mice interferes with hair follicle development.
Wysolmerski JJ, Broadus AE, Zhou J, Fuchs E, Milstone LM, Philbrick WM. Overexpression of parathyroid hormone-related protein in the skin of transgenic mice interferes with hair follicle development. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 1133-1137. PMID: 7508121, PMCID: PMC521468, DOI: 10.1073/pnas.91.3.1133.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsFemaleGene ExpressionHairHumansKeratinsMiceMice, TransgenicParathyroid HormoneParathyroid Hormone-Related ProteinPhenotypePromoter Regions, GeneticProteinsSkinConceptsFollicle developmentTransgenic miceHair follicle developmentHuman keratin 14 promoterParathyroid hormone-related proteinNormal hair follicle developmentOverexpression of PTHrPHormone-related proteinHormone-related peptideKeratin 14 promoterHumoral hypercalcemiaPTHrP geneNormal tissuesMicePTHrPSkinOverexpressionCellular differentiationEarly stagesHypercalcemiaMalignancySyndromePeptidesFollicles
1992
Parathyroid hormone-related protein. Evidence for secretion of a novel mid-region fragment by three different cell types.
Soifer NE, Dee KE, Insogna KL, Burtis WJ, Matovcik LM, Wu TL, Milstone LM, Broadus AE, Philbrick WM, Stewart AF. Parathyroid hormone-related protein. Evidence for secretion of a novel mid-region fragment by three different cell types. Journal Of Biological Chemistry 1992, 267: 18236-18243. PMID: 1517251, DOI: 10.1016/s0021-9258(19)37178-9.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCarcinoma, Renal CellCell LineElectrophoresis, Polyacrylamide GelFluorescent Antibody TechniqueHumansImmunohistochemistryKidney NeoplasmsKineticsMolecular Sequence DataParathyroid HormoneParathyroid Hormone-Related ProteinPeptide FragmentsProtein BiosynthesisProteinsTransfectionConceptsMid-region fragmentCell typesSecretory formBasic amino acid motifHormone-related proteinAmino acid motifsImportant biological functionsAmino acid sequenceExtensive post-translational processingPost-translational processingDifferent cell typesHuman renal carcinoma cellsInterspecies conservationSodium dodecyl sulfate-polyacrylamide gel electrophoresis analysisRenal carcinoma cellsAcid motifGel electrophoresis analysisAcid sequenceBiological functionsParathyroid hormone-related proteinRegion-specific immunoassaysRat insulinoma cellsCell extractsCleavage siteAmino acidsAnalysis of PTHRP binding and signal transduction mechanisms in benign and malignant squamous cells
Orloff J, Ganz M, Ribaudo A, Burtis W, Reiss M, Milstone L, Stewart A. Analysis of PTHRP binding and signal transduction mechanisms in benign and malignant squamous cells. American Journal Of Physiology 1992, 262: e599-e607. PMID: 1590371, DOI: 10.1152/ajpendo.1992.262.5.e599.Peer-Reviewed Original ResearchMeSH KeywordsCalciumCarcinoma, Squamous CellCarrier ProteinsCross-Linking ReagentsHumansKeratinocytesParathyroid HormoneParathyroid Hormone-Related ProteinPeptide FragmentsProteinsSignal TransductionSkinTumor Cells, CulturedConceptsParathyroid hormone-related proteinHuman epidermal keratinocytesIntracellular calciumImmunoreactive parathyroid hormone-related proteinLow-affinity binding sitesSqCC cellsDose-dependent transient increaseHuman parathyroid hormone-related proteinMalignant squamous cellsHormone-related proteinIntracellular calcium responseCyclic monophosphate responsePotential autocrine roleSquamous carcinoma cellsHigh-affinity receptorHalf maximal inhibitory concentration (IC50) valuesPTHrP concentrationsSquamous cellsPTH receptorIsoproterenol responseAutocrine roleCalcium responsePTHrP bindingMonophosphate responseInhibitory concentration values
1991
Glycosylation of Parathyroid Hormone-Related Peptide Secreted by Human Epidermal Keratinocytes*
WU T, SOIFER N, BURTIS W, MILSTONE L, STEWART A. Glycosylation of Parathyroid Hormone-Related Peptide Secreted by Human Epidermal Keratinocytes*. The Journal Of Clinical Endocrinology & Metabolism 1991, 73: 1002-1007. PMID: 1939513, DOI: 10.1210/jcem-73-5-1002.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternCells, CulturedChromatography, AffinityChromatography, High Pressure LiquidElectrophoresis, Polyacrylamide GelEpidermisGlycosylationHumansInfant, NewbornKeratinocytesMaleParathyroid Hormone-Related ProteinProtein Processing, Post-TranslationalProteinsRecombinant ProteinsImmunoaffinity purification of parathyroid hormone‐related protein from bovine milk and human keratinocyte‐conditioned medium
Stewart A, Wu T, Insogna K, Milstone L, Burtis W. Immunoaffinity purification of parathyroid hormone‐related protein from bovine milk and human keratinocyte‐conditioned medium. Journal Of Bone And Mineral Research 1991, 6: 305-311. PMID: 2035357, DOI: 10.1002/jbmr.5650060313.Peer-Reviewed Original ResearchAnimalsChromatography, AffinityChromatography, High Pressure LiquidCulture MediaHumansKeratinocytesMilkNeoplasm ProteinsParathyroid Hormone-Related ProteinProteinsImmunohistochemical Localization of Parathyroid Hormone-Related Protein (PTHRP) In Normal Human Skin
Atillasoy E, Burtis W, Milstone L. Immunohistochemical Localization of Parathyroid Hormone-Related Protein (PTHRP) In Normal Human Skin. Journal Of Investigative Dermatology 1991, 96: 277-280. PMID: 1991989, DOI: 10.1111/1523-1747.ep12464480.Peer-Reviewed Original ResearchMeSH KeywordsAntibodiesCells, CulturedEpidermal CellsHumansImmunoenzyme TechniquesKeratinocytesParathyroid HormoneParathyroid Hormone-Related ProteinProteinsRadioimmunoassayReference ValuesSkinConceptsParathyroid hormone-related peptideNormal human skinHormone-related peptideHuman keratinocytesHuman skinParathyroid hormoneSquamous differentiationAffinity-purified polyclonal antibodiesNumber of autocrineImmunohistochemical localizationParacrine functionEpithelial cellsViable portionKeratinocytesSkinPolyclonal antibodiesRecent studiesPeptidesAutocrineHormoneAntibodies
1989
Native and a synthetic analogue of the malignancy-associated parathyroid hormone-like protein have in vitro transforming growth factor-like properties.
Insogna KL, Stewart AF, Morris CA, Hough LM, Milstone LM, Centrella M. Native and a synthetic analogue of the malignancy-associated parathyroid hormone-like protein have in vitro transforming growth factor-like properties. Journal Of Clinical Investigation 1989, 83: 1057-1060. PMID: 2537846, PMCID: PMC303783, DOI: 10.1172/jci113947.Peer-Reviewed Original ResearchMeSH KeywordsCell Transformation, NeoplasticCyclic AMPEpidermal Growth FactorFibroblastsFibronectinsHumansNeoplasm ProteinsParathyroid HormoneParathyroid Hormone-Related ProteinTransforming Growth FactorsConceptsGrowth factor-like propertiesParathyroid hormone-like proteinHormone-like proteinNRK-49F cellsParaneoplastic syndromeHumoral hypercalcemiaLatter hormoneHuman tumorsPhysiological actionsPTHHuman dermal fibroblastsSignificant increaseSoft agarDisplay actionsDermal fibroblastsSynthetic peptidesSynthetic analoguesHypercalcemiaMalignancySyndromeTumorsUnique primary structure
1988
Two distinct tumor-derived, parathyroid hormone-like peptides result from alternative ribonucleic acid splicing.
Mangin M, Ikeda K, Dreyer B, Milstone L, Broadus A. Two distinct tumor-derived, parathyroid hormone-like peptides result from alternative ribonucleic acid splicing. Endocrinology 1988, 2: 1049-55. PMID: 2464747, DOI: 10.1210/mend-2-11-1049.Peer-Reviewed Original Research