2015
Gene expression profiling in pachyonychia congenita skin
Cao YA, Hickerson RP, Seegmiller BL, Grapov D, Gross MM, Bessette MR, Phinney BS, Flores MA, Speaker TJ, Vermeulen A, Bravo AA, Bruckner AL, Milstone LM, Schwartz ME, Rice RH, Kaspar RL. Gene expression profiling in pachyonychia congenita skin. Journal Of Dermatological Science 2015, 77: 156-165. PMID: 25656049, PMCID: PMC4374015, DOI: 10.1016/j.jdermsci.2015.01.001.Peer-Reviewed Original ResearchConceptsPachyonychia congenitaTape-strip samplesPlantar skinSerine protease inhibitorNon-PC controlsPainful plantar keratodermaSkin barrier homeostasisPC patientsPatient groupControl volunteersMajor symptomsSkin biopsiesPC pathogenesisPlantar keratodermaPathogenic sequelaeSkin disordersBarrier homeostasisPatient samplesGene expression profilingKeratinocyte proliferationEnvelope proteinBiopsyMRNA analysisProtease inhibitorsSkin
2010
Development of Quantitative Molecular Clinical End Points for siRNA Clinical Trials
Hickerson RP, Leachman SA, Pho LN, Gonzalez-Gonzalez E, Smith FJ, McLean WH, Contag CH, Leake D, Milstone LM, Kaspar RL. Development of Quantitative Molecular Clinical End Points for siRNA Clinical Trials. Journal Of Investigative Dermatology 2010, 131: 1029-1036. PMID: 21191405, DOI: 10.1038/jid.2010.372.Peer-Reviewed Original ResearchConceptsClinical trialsPachyonychia congenitaEnd pointPhase 1b clinical trialClinical end pointsRare skin disorderMolecular end pointsTypes of trialsImmune surveillancePatient-derived keratinocytesSkin disordersSustained inhibitionTreatment resultsRNAi-based therapeuticsRNA interferenceMRNA levelsTrialsTotal RNAHuman skinKeratinocyte culturesMRNAAmplifiable mRNATherapeuticsInhibitorsInhibition
1996
CD44 Expression on Epidermal Melanocytes
Herbold K, Zhou J, Haggerty J, Milstone L. CD44 Expression on Epidermal Melanocytes. Journal Of Investigative Dermatology 1996, 106: 1230-1235. PMID: 8752662, DOI: 10.1111/1523-1747.ep12348871.Peer-Reviewed Original Research
1988
Two distinct tumor-derived, parathyroid hormone-like peptides result from alternative ribonucleic acid splicing.
Mangin M, Ikeda K, Dreyer B, Milstone L, Broadus A. Two distinct tumor-derived, parathyroid hormone-like peptides result from alternative ribonucleic acid splicing. Endocrinology 1988, 2: 1049-55. PMID: 2464747, DOI: 10.1210/mend-2-11-1049.Peer-Reviewed Original ResearchIdentification of a cDNA encoding a parathyroid hormone-like peptide from a human tumor associated with humoral hypercalcemia of malignancy.
Mangin M, Webb A, Dreyer B, Posillico J, Ikeda K, Weir E, Stewart A, Bander N, Milstone L, Barton D. Identification of a cDNA encoding a parathyroid hormone-like peptide from a human tumor associated with humoral hypercalcemia of malignancy. Proceedings Of The National Academy Of Sciences Of The United States Of America 1988, 85: 597-601. PMID: 2829195, PMCID: PMC279598, DOI: 10.1073/pnas.85.2.597.Peer-Reviewed Original ResearchConceptsParathyroid hormone-like peptideHumoral hypercalcemiaHormone-like peptideCommon paraneoplastic syndromeTumor-derived peptidesHuman renal carcinomaParaneoplastic syndromeNormal human keratinocytesHuman PTHRenal carcinomaHuman tumorsSyndromeHuman keratinocytesHypercalcemiaMalignancyBlot analysisTumorsRNA blot hybridization analysisPartial N-terminal amino acid sequencePeptidesMRNABlot hybridization analysisMature peptideCarcinomaPTH
1977
δ-Crystallin gene expression in embryonic chick lens epithelia cultured in the presence of insulin
Milstone L, Piatigorsky J. δ-Crystallin gene expression in embryonic chick lens epithelia cultured in the presence of insulin. Experimental Cell Research 1977, 105: 9-14. PMID: 837998, DOI: 10.1016/0014-4827(77)90147-1.Peer-Reviewed Original ResearchConceptsEmbryonic lens epitheliumΔ-crystallin synthesisMessenger RNAΔ-crystallinGene expressionLens epitheliumPresence of insulinCultured lens epitheliumFetal calf serumChick lens epitheliumLens epithelial cellsCell elongationTranslational efficiencyCalf serumPolyacrylamide gel electrophoresisTotal protein synthesisProtein synthesisMolecular hybridizationGel electrophoresisSodium dodecyl sulfate-gradient polyacrylamide gel electrophoresisEpithelial cellsSynthesis increasesCellsExpressionHours of culture
1976
δ-Crystallin mRNA in chick lens cells: mRNA accumulates during differential stimulation of δ-crystallin synthesis in cultured cells
Milstone L, Zelenka P, Piatigorsky J. δ-Crystallin mRNA in chick lens cells: mRNA accumulates during differential stimulation of δ-crystallin synthesis in cultured cells. Developmental Biology 1976, 48: 197-204. PMID: 1254080, DOI: 10.1016/0012-1606(76)90084-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCells, CulturedChick EmbryoCrystallinsDNALens, CrystallineRNA, MessengerConceptsΔ-crystallin synthesisChick lens epithelial cellsChick lens cellsEmbryonic lens epitheliumLens fiber cellsLens epithelial cellsFiber cellsLens cellsDifferential stimulationProtein synthesisCultured cellsMRNALens epitheliumEpithelial cellsTissue cultureCellsDifferentiationOverall stimulationAccumulationStimulationSpecializationCultureSynthesisOvo