1991
Glycosylation of Parathyroid Hormone-Related Peptide Secreted by Human Epidermal Keratinocytes*
WU T, SOIFER N, BURTIS W, MILSTONE L, STEWART A. Glycosylation of Parathyroid Hormone-Related Peptide Secreted by Human Epidermal Keratinocytes*. The Journal Of Clinical Endocrinology & Metabolism 1991, 73: 1002-1007. PMID: 1939513, DOI: 10.1210/jcem-73-5-1002.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternCells, CulturedChromatography, AffinityChromatography, High Pressure LiquidElectrophoresis, Polyacrylamide GelEpidermisGlycosylationHumansInfant, NewbornKeratinocytesMaleParathyroid Hormone-Related ProteinProtein Processing, Post-TranslationalProteinsRecombinant ProteinsConceptsBioactive speciesTrifluoromethanesulfonic acidSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresis
1990
Metalloproteases of infective Ancylostoma hookworm larvae and their possible functions in tissue invasion and ecdysis
Hotez P, Haggerty J, Hawdon J, Milstone L, Gamble H, Schad G, Richards F. Metalloproteases of infective Ancylostoma hookworm larvae and their possible functions in tissue invasion and ecdysis. Infection And Immunity 1990, 58: 3883-3892. PMID: 2254016, PMCID: PMC313750, DOI: 10.1128/iai.58.12.3883-3892.1990.Peer-Reviewed Original ResearchConceptsProtease activitySodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisProtein substratesSulfate-polyacrylamide gel electrophoresisMr polypeptideNematode Strongyloides stercoralisBovine elastinPossible functionsBiochemical similaritiesAlkaline pH optimumLarvaeConnective tissue macromoleculesHuman hookworms Ancylostoma duodenaleInvasion propertiesGel electrophoresisEnzyme activityAncylostoma speciesBiochemical differencesA. caninum larvaeMinor bandsProtease compositionSmall peptidesHuman fibronectinMajor band