Methotrexate induces differentiation of human keratinocytes.
Schwartz P, Barnett S, Atillasoy E, Milstone L. Methotrexate induces differentiation of human keratinocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 594-598. PMID: 1731329, PMCID: PMC48285, DOI: 10.1073/pnas.89.2.594.Peer-Reviewed Original ResearchConceptsEffect of methotrexateAbility of methotrexateProliferation of keratinocytesProportion of cellsMarkers of differentiationHuman epidermal keratinocytesMethotrexateTherapeutic actionEpidermal keratinocytesMarked increaseHuman keratinocytesKeratinocytesEnvelope proteinSDS/PAGE profilesInvolucrin synthesisNormal cellsTerminal differentiationTissue homeostasisThymine deoxyribonucleotidesDifferentiationCellsPsoriasisCell morphologyReproducible changesDose