Metalloproteases of infective Ancylostoma hookworm larvae and their possible functions in tissue invasion and ecdysis
Hotez P, Haggerty J, Hawdon J, Milstone L, Gamble H, Schad G, Richards F. Metalloproteases of infective Ancylostoma hookworm larvae and their possible functions in tissue invasion and ecdysis. Infection And Immunity 1990, 58: 3883-3892. PMID: 2254016, PMCID: PMC313750, DOI: 10.1128/iai.58.12.3883-3892.1990.Peer-Reviewed Original ResearchConceptsProtease activitySodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisProtein substratesSulfate-polyacrylamide gel electrophoresisMr polypeptideNematode Strongyloides stercoralisBovine elastinPossible functionsBiochemical similaritiesAlkaline pH optimumLarvaeConnective tissue macromoleculesHuman hookworms Ancylostoma duodenaleInvasion propertiesGel electrophoresisEnzyme activityAncylostoma speciesBiochemical differencesA. caninum larvaeMinor bandsProtease compositionSmall peptidesHuman fibronectinMajor band