1994
Overexpression of parathyroid hormone-related protein in the skin of transgenic mice interferes with hair follicle development.
Wysolmerski JJ, Broadus AE, Zhou J, Fuchs E, Milstone LM, Philbrick WM. Overexpression of parathyroid hormone-related protein in the skin of transgenic mice interferes with hair follicle development. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 1133-1137. PMID: 7508121, PMCID: PMC521468, DOI: 10.1073/pnas.91.3.1133.Peer-Reviewed Original ResearchConceptsFollicle developmentTransgenic miceHair follicle developmentHuman keratin 14 promoterParathyroid hormone-related proteinNormal hair follicle developmentOverexpression of PTHrPHormone-related proteinHormone-related peptideKeratin 14 promoterHumoral hypercalcemiaPTHrP geneNormal tissuesMicePTHrPSkinOverexpressionCellular differentiationEarly stagesHypercalcemiaMalignancySyndromePeptidesFollicles
1992
Parathyroid hormone-related protein. Evidence for secretion of a novel mid-region fragment by three different cell types.
Soifer NE, Dee KE, Insogna KL, Burtis WJ, Matovcik LM, Wu TL, Milstone LM, Broadus AE, Philbrick WM, Stewart AF. Parathyroid hormone-related protein. Evidence for secretion of a novel mid-region fragment by three different cell types. Journal Of Biological Chemistry 1992, 267: 18236-18243. PMID: 1517251, DOI: 10.1016/s0021-9258(19)37178-9.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCarcinoma, Renal CellCell LineElectrophoresis, Polyacrylamide GelFluorescent Antibody TechniqueHumansImmunohistochemistryKidney NeoplasmsKineticsMolecular Sequence DataParathyroid HormoneParathyroid Hormone-Related ProteinPeptide FragmentsProtein BiosynthesisProteinsTransfectionConceptsMid-region fragmentCell typesSecretory formBasic amino acid motifHormone-related proteinAmino acid motifsImportant biological functionsAmino acid sequenceExtensive post-translational processingPost-translational processingDifferent cell typesHuman renal carcinoma cellsInterspecies conservationSodium dodecyl sulfate-polyacrylamide gel electrophoresis analysisRenal carcinoma cellsAcid motifGel electrophoresis analysisAcid sequenceBiological functionsParathyroid hormone-related proteinRegion-specific immunoassaysRat insulinoma cellsCell extractsCleavage siteAmino acidsAnalysis of PTHRP binding and signal transduction mechanisms in benign and malignant squamous cells
Orloff J, Ganz M, Ribaudo A, Burtis W, Reiss M, Milstone L, Stewart A. Analysis of PTHRP binding and signal transduction mechanisms in benign and malignant squamous cells. American Journal Of Physiology 1992, 262: e599-e607. PMID: 1590371, DOI: 10.1152/ajpendo.1992.262.5.e599.Peer-Reviewed Original ResearchConceptsParathyroid hormone-related proteinHuman epidermal keratinocytesIntracellular calciumImmunoreactive parathyroid hormone-related proteinLow-affinity binding sitesSqCC cellsDose-dependent transient increaseHuman parathyroid hormone-related proteinMalignant squamous cellsHormone-related proteinIntracellular calcium responseCyclic monophosphate responsePotential autocrine roleSquamous carcinoma cellsHigh-affinity receptorHalf maximal inhibitory concentration (IC50) valuesPTHrP concentrationsSquamous cellsPTH receptorIsoproterenol responseAutocrine roleCalcium responsePTHrP bindingMonophosphate responseInhibitory concentration values